Polycyclic aromatic hydrocarbons (PAHs) are a group of more than hundred compounds that are ubiquitous in our environment. Some of these PAHs are known to be carcinogenic, mutagenic and teratogenic. PAHs have been detected in dried herbs that were cultured in The Netherlands as well as in other European countries above the maximum levels in dried herbs set by the EU (EU, 2015) for benzo(a)pyrene and the sum of the following four PAHs benzo(a)pyrene, benzo[a]anthracene, benzo[b]fluoranteen and chrysene. The origin of these PAHs in herbs is unknown. VNK cultivates, harvests and dries herbs including valerian and would like to identify the source of PAHs to comply to the EU limits for PAHs in herbs. The goal of the present study was to identify the source of PAHs found in valerian root, and to identify possible measures to reduce the concentration of PAHs in valerian root.
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Worldwide, plastic cups are used for serving drinks. Some typical examples of large-scale consumption are large concerts and festivals. As a part of the BIOCAS project, which focusses on the valorization of biomass through various routes, a PHA biobased festival cup was developed and created to reduce the impact of current fossil plastics. The role of VHL was to assess the environmental impact. The aim of the report is to inform the BIOCAS-partners about the use of plastic cups, and address the environmental impact in comparison with other types of biobased plastic cups and fossil-based cups. This report can serve as a basis for making choices within all different types of (plastic/biobased) cups. Besides, it can be used as a public communication tool about the environmental impact of different types of (plastic/biobased) cup applications.
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Dark homogenous fungal-based layers called biofinishes and vegetable oils are keyingredients of an innovative wood protecting system. The aim of this study was todetermine which of the vegetable oils that have been used to generate biofinishes onwood will provide carbon and energy for the biofinish-inhabiting fungus Aureobasidiummelanogenum, and to determine the effect of the oil type and the amount of oil on thecell yield. Aureobasidium melanogenum was cultivated in shake flasks with differenttypes and amounts of carbon-based nutrients. Oil-related total cell and colony-formingunit growth were demonstrated in suspensions with initially 1% raw linseed,stand linseed, and olive oil. Oil-related cell growth was also demonstrated with rawlinseed oil, using an initial amount of 0.02% and an oil addition during cultivation. Nilered staining showed the accumulation of fatty acids inside cells grown in the presenceof oil. In conclusion, each tested vegetable oil was used as carbon and energysource by A. melanogenum. The results indicated that stand linseed oil provides lesscarbon and energy than olive and raw linseed oil. This research is a fundamental stepin unraveling the effects of vegetable oils on biofinish formation.
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It is known that several bacteria in sewage treatment plants can produce attractive quantities of biodegradable polymers within their cell walls (up to 80% of the cell weight). These polymers may consist of polyhydroxyalkanoates (PHA), a bioplastic which exhibits interesting characteristics like excellent biodegradation, low melting point and good environmental footprint. PHA bioplastics or PHBV are still quite expensive because cumbersome downstream processing steps of the PHAcontaining bacteria are needed before PHA can be applied in products. In this proposal, the consortium investigates the possibilities for eliminating these expensive and environmentally intensive purification steps, and as a result contribute to speeding up the up-take of PHA production of residual streams by the market. The objective of the project is to investigate the possibilities of direct extrusion of PHAcontaining bacteria and the application opportunities of the extruded PHA. The consortium of experienced partners (Paques Biomaterials, MAAN Group, Ecoras and CoEBBE) will investigate and test the extrusion of different types of PHA-containing biomass, and analyse the products on composition, appearance and mechanical properties. Moreover, the direct extrusion process will be evaluated and compared with conventional PHA extraction and subsequent extrusion. The expected result will be a proof of principle and provide an operational window for the application of direct extrusion with PHA-containing biomass produced using waste streams, either used as such or in blends with purified PHA. Both the opportunities of the direct extrusion process itself as well as the application opportunities of the extruded PHA will be mapped. If the new process leads to a cheaper, more environmentally friendly produced and applicable PHA, the proof of principle developed by the consortium could be the first step in a larger scale development that could help speeding up the implementation of the technology for PHA production from residual streams in the market.
