Forensic and behavioural science are often seen as two different disciplines. However, there is a growing realization that the two disciplines should be more strongly integrated. Incorporating psychological theories on human behaviour in forensic science could help solving investigative problems, especially at the crime scene. At the crime scene it is not just about applying scientific methods to analyse traces; these traces must first be perceived and categorized as relevant. At the crime scene, the behavioural perspective of an investigative psychologist could play an important role. In this study, we examine to what extent (1) investigative psychologists detect deviant behavioural cues compared to forensic examiners when investigating a crime scene, (2) forensic examiners can find the relevant traces that can be associated with this behaviour and (3) the availability of a psychological report highlighting these behavioural cues helps forensic examiners in finding more relevant traces. To this end, a total of 14 investigative psychologists and 40 forensic examiners investigated a virtual 3D mock crime scene. The results of this study show that investigative psychologists see significantly more deviant behavioural cues than forensic examiners, and that forensic examiners who receive a psychological report on these cues recognize and collect significantly more traces that can be linked to deviant behaviour and have a high evidential value than examiners who did not receive this information. However, the study also demonstrates that behavioural information is likely to be ignored when it contradicts existing beliefs.
Knowledge of the time of deposition is pivotal in forensic investigations. Recent studies show that changes in intrinsic fluorescence over time can be used to estimate the age of body fluids. These changes have been attributed to oxidative modifications caused by protein–lipid interactions. This pilot study aims to explore the impact of these modifications on body fluid fluorescence, enhancing the protein–lipid model system for age estimation. Lipid and protein oxidation markers, including protein carbonyls, dityrosine, advanced glycation end-products (AGEs), malondialdehyde (MDA), and 4-hydroxynonenal (HNE), were studied in aging semen, urine, and saliva over 21 days. Surface plasmon resonance imaging (SPRi), enzyme-linked immunosorbent assay (ELISA), and fluorescence spectroscopy were applied. Successful detection of AGE, dityrosine, MDA, and HNE occurred in semen and saliva via SPRi, while only dityrosine was detected in urine. Protein carbonyls were measured in all body fluids, but only in saliva was a significant increase observed over time. Additionally, protein fluorescence loss and fluorescent oxidation product formation were assessed, showing significant decreases in semen and saliva, but not in urine. Although optimization is needed for accurate quantification, this study reveals detectable markers for protein and lipid oxidation in aging body fluids, warranting further investigation.
MULTIFILE
Already for some decades lateral flow assays (LFAs) are ‘common use’ devices in our daily life. Also, for forensic use LFAs are developed, such as for the analysis of illicit drugs and DNA, but also for the detection of explosives and body fluid identification. Despite their advantages, including ease-of-use, LFAs are not yet frequently applied at a crime scene. This review describes (academic) developments of LFAs for forensic applications, focusing on biological and chemical applications, whereby the main advantages and disadvantages of LFAs for the different forensic applications are summarized. Additionally, a critical review is provided, discussing why LFAs are not frequently applied within the forensic field and highlighting the steps that are needed to bring LFAs to the forensic market.
