Abstract: Aureobasidium is omnipresent and can be isolated from air, water bodies, soil, wood, and other plant materials, as well as inorganic materials such as rocks and marble. A total of 32 species of this fungal genus have been identified at the level of DNA, of which Aureobasidium pullulans is best known. Aureobasidium is of interest for a sustainable economy because it can be used to produce a wide variety of compounds, including enzymes, polysaccharides, and biosurfactants. Moreover, it can be used to promote plant growth and protect wood and crops. To this end, Aureobasidium cells adhere to wood or plants by producing extracellular polysaccharides, thereby forming a biofilm. This biofilm provides a sustainable alternative to petrol-based coatings and toxic chemicals. This and the fact that Aureobasidium biofilms have the potential of self-repair make them a potential engineered living material avant la lettre. Key points: •Aureobasidium produces products of interest to the industry •Aureobasidium can stimulate plant growth and protect crops •Biofinish of A. pullulans is a sustainable alternative to petrol-based coatings •Aureobasidium biofilms have the potential to function as engineered living materials.
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Abstract Aureobasidium is omnipresent and can be isolated from air, water bodies, soil, wood, and other plant materials, as well as inorganic materials such as rocks and marble. A total of 32 species of this fungal genus have been identified at the level of DNA, of which Aureobasidium pullulans is best known. Aureobasidium is of interest for a sustainable economy because it can be used to produce a wide variety of compounds, including enzymes, polysaccharides, and biosurfactants. Moreover, it can be used to promote plant growth and protect wood and crops. To this end, Aureobasidium cells adhere to wood or plants by producing extracellular polysaccharides, thereby forming a biofilm. This biofilm provides a sustainable alternative to petrol-based coatings and toxic chemicals. This and the fact that Aureobasidium biofilms have the potential of self-repair make them a potential engineered living material avant la lettre.
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Eight new primer sets were designed for PCR detection of (i) mono-oxygenase and dioxygenase gene sequences involved in initial attack of bacterial aerobic BTEX degradation and of (ii) catechol 2,3-dioxygenase gene sequences responsible for meta-cleavage of the aromatic ring. The new primer sets allowed detection of the corresponding genotypes in soil with a detection limit of 10(3)-10(4) or 10(5)-10(6) gene copies g(-1) soil, assuming one copy of the gene per cell. The primer sets were used in PCR to assess the distribution of the catabolic genes in BTEX degrading bacterial strains and DNA extracts isolated from soils sampled from different locations and depths (vadose, capillary fringe and saturated zone) within a BTEX contaminated site. In both soil DNA and the isolates, tmoA-, xylM- and xylE1-like genes were the most frequently recovered BTEX catabolic genes. xylM and xylE1 were only recovered from material from the contaminated samples while tmoA was detected in material from both the contaminated and non-contaminated samples. The isolates, mainly obtained from the contaminated locations, belonged to the Actinobacteria or Proteobacteria (mainly Pseudomonas). The ability to degrade benzene was the most common BTEX degradation phenotype among them and its distribution was largely congruent with the distribution of the tmoA-like genotype. The presence of tmoA and xylM genes in phylogenetically distant strains indicated the occurrence of horizontal transfer of BTEX catabolic genes in the aquifer. Overall, these results show spatial variation in the composition of the BTEX degradation genes and hence in the type of BTEX degradation activity and pathway, at the examined site. They indicate that bacteria carrying specific pathways and primarily carrying tmoA/xylM/xylE1 genotypes, are being selected upon BTEX contamination.
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The research presented in this thesis has highlighted (bio)geochemical, hydrological, and wetland ecological processes that interact and enhance ecosystem development on wetlands built on fine sediment. A combination of greenhouse and laboratory experiments were conducted. Some measured data from these experiments formed important input for subsequent analysis in a modeling environment. The findings presented in Chapters 2-6 can be divided into four topics: 1) Plant–soil interactions in the terrestrial zone, 2) wetland–terrestrial processes influencing nutrient availability in the land–water zone, 3) effects of plants on sediment consolidation in the terrestrial zone, and 4) effects of bioturbation on nutrient availability in the aquatic zone. The next sections give a summary of the results for these four topics. The last section summarizes the recommendations formulated for the Marker Wadden project.
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Inulin is a soluble dietary fibre, also classified as a prebiotic, extracted from chicory roots. The present study aimed to determine the effect of consumption of native chicory inulin on the stool frequency of middle-aged to older adults (40–75 years old) with uncomfortably but not clinically relevant low stool frequency, specified as two to four days without bowel movements per week. Two randomised, double blind, placebo-controlled crossover trials were conducted using similar protocols in differing populations. Trial A was conducted in Amsterdam, The Netherlands and subsequently Trial B was conducted in Newcastle, United Kingdom. Both trials involved supplementation for 5 weeks with 10 g per day of inulin or placebo, a washout period of 2 weeks, and then crossed over to receive the other treatment. In Trial B, faecal gut microbiota composition was assessed using 16S rRNA gene sequencing. In Trial A, which 10 volunteers completed, the stool frequency was significantly increased to an average 4.9 ± 0.23 (SEM) times per week during inulin periods versus 3.6 ± 0.25 in the periods with placebo (p = 0.01). In contrast, in Trial B which 20 volunteers completed, there was no significant effect of the inulin on stool frequency (7.5 ± 2.1 times per week with inulin, 8.1 ± 3.0 with placebo, p = 0.35). However, many subjects in Trial B had a stool frequency >5 per week also for the placebo period, in breach of the inclusion criteria. Combining the data of 16 low stool frequency subjects from Trials A and B showed a significant effect of inulin to increase stool frequency from 4.1 to 5.0 per week (p = 0.032). Regarding secondary outcomes, stool consistency was significantly softer with inulin treatment compared to placebo periods, it increased 0.29 on the Bristol stool scale (p = 0.008) when data from all subjects of Trials A and B were combined. No other differences in bowel habit parameters due to inulin consumption were significant. None of the differences in specific bacterial abundance, alpha or beta diversity were significant, however the trends were in directions consistent with published studies on other types of inulin. We conclude that 10 g per day of native chicory inulin can increase stool frequency in subjects with low stool frequency.
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De EU en Nederland streven naar significante reducties in broeikasgas- en stikstofemissies tegen 2030. De Nederlandse melkveehouderij draagt met respectievelijk 36% en 48% bij aan de broeikasgasuitstoot en de ammoniakemissies van de landbouw. Het aanzuren van mest met zwavelzuur, zoals in Denemarken, is effectief maar kostbaar. Dit onderzoek evalueert de haalbaarheid van biologisch aanzuren van dierlijke mest met melasse als alternatief, met als doel het verminderen van emissies en het verbeteren van mestverwaarding.Op een melkveehouderij in Someren met circa 100 koeien werd een experiment uitgevoerd waarbij een kleinere mestkelder werd biologisch aangezuurd met melasse en een grotere kelder als controle diende. Beide kelders werden op 6 november 2023 nagenoeg leeggepompt en aan de kleine kelder werd 12% melasse (op initiële hoeveelheid mest basis) toegevoegd. De pH in de aangezuurde mestkelder daalde van 8,1 naar 5,5 in 13 dagen en stabiliseerde op 4,7. Na 9 weken steeg de pH naar 6,0. De aangezuurde dunne fractie bevatte significant meer fosfaat (61%) en de biogasopbrengst steeg met 43% ten opzichte van de controle.Dit onderzoek bevestigt de hypothese dat het toevoegen van organische (rest)stromen aan mestkelders effectief is om methaan- en ammoniakemissies te verlagen en de mestverwaarding te verbeteren. Daarnaast is de aanpak passend binnen de huidige bedrijfsvoering inclusief de regelgeving. De biogasproductie nam significant toe waardoor de mestverwaarding verbeterde.Aanbevolen wordt om de pH van drijfmest rond 5,5 te houden, het verzuringsproces eventueel op te starten met een organisch zuur, en verder onderzoek te doen naar optimale melassedosering, frequenter mengen, en inzet van alternatieve suikerrijke resstromen. Voor borging van het proces zijn implementatie van een pH-monitoringssysteem en aanvullende emissiemetingen noodzakelijk.
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Inaugurele rede uitgesproken op 9 mei 2019 door Dr. J.E. (Judith) van de Mortel bij de benoeming tot lector “Gezonde plant op een vitale en duurzame bodem” aan HAS Hogeschool Venlo. De krant werd uitgegeven ter ere van de inaugurele rede. Dit deed ze op een bijzondere manier: alle genodigden gingen met een denkbeeldige tijdmachine vooruit naar het jaar 2030 waar ze Judith ontmoetten. Judith keek vervolgens terug naar het jaar 2019 en stelde dat ze hoopt dat de sector in de tussenliggende jaren bereikt heeft dat alle agrarische bodems in Nederland duurzaam beheerd worden door verschillende aanpakken te combineren. Om dit te illustreren schetste ze een beeld van hoe de agrarische onderneming er in 2030 uitziet. Met het lectoraat wil ze hieraan bijdragen. De krant is dan ook geschreven alsof het 9 mei 2030 is.
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Closed loop or ‘circular’ production systems known as Circular Economy and Cradle to Cradle represent a unique opportunity to radically revise the currently wasteful system of production. One of the challenges of such systems is that circular products need to be both produced locally with minimum environmental footprint and simultaneously satisfy demand of global consumers. This article presents a literature review that describes the application of circular methodologies to education for sustainability, which has been slow to adopt circular systems to the curriculum. This article discusses how Bachelor and Master-level students apply their understanding of these frameworks to corporate case studies. Two assignment-related case studies are summarized, both of which analyze products that claim to be 'circular'. The students' research shows that the first case, which describes the impact of a hybrid material soda bottle, does not meet circularity criteria. The second case study, which describes products and applications of a mushroom-based material, is more sustainable. However, the students' research shows that the manufacturers have omitted transport from the environmental impact assessment and therefore the mushroom materials may not be as sustainable as the manufacturers claim. As these particular examples showed students how green advertising can be misleading, applying “ideal” circularity principles as part of experiential learning could strengthen the curriculum. Additionally, this article recommends that sustainable business curriculum should also focus on de-growth and steady-state economy, with these radical alternatives to production becoming a central focus of education of responsible citizens. https://doi.org/10.1016/j.jclepro.2019.02.005 LinkedIn: https://www.linkedin.com/in/helenkopnina/
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De afgelopen 10 jaar heeft Prof.Dr. Olaf van Kooten als lector Duurzame verbindingen in de Greenport bij Hogeschool Inholland, Research & Innovation Centre Agri, Food & Life Sciences, meegewerkt aan bijzondere ontwikkelingen in de tuinbouwsector. De ontwikkelingen in het huidige onderzoek zijn bepalend voor de tuinbouw in de komende decennia. Een systemische visie is noodzakelijk voor het creëren van een duurzame toekomst, waarin de tuinbouw haar belangrijke functie kan vervullen in evenwicht met de natuur en de samenleving.
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Poor well-being amongst older adults poses a serious health concern. Simultaneously, research shows that contact with nature can improve various facets of well-being, including physical, social, and mental well-being. However, nature is not always accessible for older adults due to mobility restrictions and related care needs that come with age. A promising strategy aims at bringing nature inside through pervasive technologies. However, so far, there is little academic understanding of essential nature characteristics, psychological processes involved, and means for implementation in practice. The current study used a three-folded rapid review to assess current understanding and strategies used for improving well-being for older adults through virtual reality representations of nature. Searches were performed across three databases, followed-up by content-based evaluation of abstracts. In total, a set of 25 relevant articles was identified. Only three studies specifically focus on digital nature as an intervention strategy for improving well-being amongst older adults. Although these studies provide useful starting points for the design and (technological) development of such environments, they do not generate understanding of how specific characteristics of virtual nature representations impact social well-being measures in particular, and of the underlying psychological processes involved. We conclude that follow-up research is warranted to close the gap between insights and findings from nature research, gerontology, health research, and human-technology interaction.
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