This chapter enquires into the resonance of junk news on Twitter during the campaign periods prior to the 2019 Dutch Provincial elections and European Parliamentary elections. Querying Twitter for political topics related to the two elections, and various divisive social issues such as Zwarte Piet and MH17, we analyse the spread and prominence of problematic sources. We also examined the claim that Twitter is susceptible to abuse by bot and troll-like users, and found that troll-like users were active across all political and issue spaces during the Dutch Provincial elections of 2019. Divisive issues remain steadily (even if marginally) active in junk news and tendentious news throughout the tested time frames, suggesting these issues are year-round rather than event-based or seasonal, as they are in the mainstream media.
Knowledge of the time of deposition is pivotal in forensic investigations. Recent studies show that changes in intrinsic fluorescence over time can be used to estimate the age of body fluids. These changes have been attributed to oxidative modifications caused by protein–lipid interactions. This pilot study aims to explore the impact of these modifications on body fluid fluorescence, enhancing the protein–lipid model system for age estimation. Lipid and protein oxidation markers, including protein carbonyls, dityrosine, advanced glycation end-products (AGEs), malondialdehyde (MDA), and 4-hydroxynonenal (HNE), were studied in aging semen, urine, and saliva over 21 days. Surface plasmon resonance imaging (SPRi), enzyme-linked immunosorbent assay (ELISA), and fluorescence spectroscopy were applied. Successful detection of AGE, dityrosine, MDA, and HNE occurred in semen and saliva via SPRi, while only dityrosine was detected in urine. Protein carbonyls were measured in all body fluids, but only in saliva was a significant increase observed over time. Additionally, protein fluorescence loss and fluorescent oxidation product formation were assessed, showing significant decreases in semen and saliva, but not in urine. Although optimization is needed for accurate quantification, this study reveals detectable markers for protein and lipid oxidation in aging body fluids, warranting further investigation.
