The chromatographic behavior of petanin, petunidin-3-O-[6-O-(4-O-(E)-para-coumaroyl-O-α-rhamnopyranosyl)-β-glucopyranoside]-5-O-β-glucopyranoside, is studied for the first time under conditions of reversed-phase high performance liquid chromatography in mobile phases with different pH values. The relationship between chromatographic behavior (retention time and peak efficiency) and transitions between different forms of anthocyanins is discussed. Analysis of the data obtained in the 2 to 6.5 range of mobile pH phases, the absorption spectra of petanin, and the results from studying the effect of adding tetrabutylammonium bromide to the mobile phase shows that increasing the pH results in the formation of uncharged and anionic forms, in addition to the pseudobase form.
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Semen traces are considered important pieces of evidence in forensic investigations, especially those involving sexsual offenses. Recently, our research group developed a fluorescence-based technique to accurately determine the age of semen traces. However, the specific compounds resonsible for the fluoresescent behaviour of ageing semens remain unknown. As such, in this exploratory study, the aim is to identify the components associated with the fluorescent behavior of ageing semen traces. In this investigation semen stains and various biofluorophores commonly found in body fluids were left to aged for 0, 2, 4, 7, 14 and 21 days. Subsequently, thin-layer chromatography (TLC) and ultra-performance liquid chromatography (UPLC) mass spectrometry were performed to identify the biofluorophores present in semen. Several contributors to the autofluorescence could be identified in semen stain, these include tryptophan, kynurenine, kynurenic acid, and norharman. The study sheds light on the.
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In this review, we present the growing scientific evidence showing the importance of protein and amino acid provision in nutritional support and their impact on preservation of muscle mass and patient outcomes.
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