Natural Deep Eutectic Solvents (NADES) represent a green chemistry alternative to utilization of common hazardous organic solvents. They were introduced by Abbott et al. [1], and were found to have a wide range of compositions and favorable properties. NADES are typically obtained by mixing hydrogen-bond acceptors (HBA), with hydrogen bond donors (HBD), leading to a significant depression of the melting point. The availability of components, simple preparation, biodegradability, safety, re usability and low cost are the significant advantages that call for research on their analytical applications. Three methods are most commonly used for preparing NADES: a) heating and stirring: the mixture until a clear liquid is formed; b) evaporating solvent from components solution with a rotatory evaporator; c) freeze drying of aqueous solutions.The common solvents for the extraction of anthocyanins are acidified mixtures of water with ethanol, methanol, or acetone. The anthocyanins extracts are susceptible to degradation due to high temperature, and the solvent properties (e.g. high pH) and the whole process can often be time-consuming. Extraction of anthocyanins from red cabbage by four NADES was investigated. It was demonstrated that NADES have comparable extraction efficiencies with conventional method with 0.1 M water solution of HCl. This indicates a possibility of utilization the Green chemistry extraction processes as a promising new green-extraction technology with low cost efficiency and environment friendly technology for production of safe food additives.
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The Green Biotechnology research group focusses on the application of molecular breeding/biotechnological tools and also on the development/analysis of new tools, for the breeding of enhanced vegetable crops and ornamental plants. The research group is positioned within Inholland University of Applied Sciences, Life Sciences & Chemistry and serves as a link between the breeding companies and our education of the skilled technicians of tomorrow. We are working on the development of a method for targeted mutagenesis of plant genomes using the bacterial CRISPR-Cas system. This method greatly enhances the effectiveness and speed by which new crops and plants can be developed
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By transitioning from a fossil-based economy to a circular and bio-based economy, the industry has an opportunity to reduce its overall CO2 emission. Necessary conditions for effective and significant reductions of CO2-emissions are that effective processing routes are developed that make the available carbon in the renewable sources accessible at an acceptable price and in process chains that produce valuable products that may replace fossil based products. To match the growing industrial carbon demand with sufficient carbon sources, all available circular, and renewable feedstock sources must be considered. A major challenge for greening chemistry is to find suitable sustainable carbon that is not fossil (petroleum, natural gas, coal), but also does not compete with the food or feed demand. Therefore, in this proposal, we omit the use of first generation substrates such as sugary crops (sugar beets), or starch-containing biomasses (maize, cereals).
Fungal colorants offer a sustainable alternative to synthetic colors, which are derived from fossil fuels and contribute to environmental pollution. While fungal colorants could be effectively produced through precision fermentation by microorganisms, their adoption in industry remains limited due to challenges in processing, formulation, and application. ColorFun aims to bridge the gap between laboratory research, artisanal practices, and industrial needs by developing a scalable and adaptable colorant processing system. Building on the TUFUCOL project, which focused on optimizing fungal fermentation, ColorFun consortium gears the focus to downstream processing and industrial applications by using green chemistry. Many SMEs have explored fungal colorants using traditional methods, but due to lack of consistency and reproducibility, they are unsuitable for large-scale production. Meanwhile, lab research usually does not translate directly to industrial applications. Researchers can fine-tune processes under controlled conditions while large-scale production requires consistent formulations that work across different material substrates and processing environments. Without bridging these gaps, fungal colorants remain confined to research and small-scale applications rather than becoming viable industrial alternatives. Instead of developing separate solutions for each sector, ColorFun is working towards a set of standardized extraction and stabilization methods for a stable base colorant product. This pre-processed colorant can then be adjusted by different industries to meet their specific needs. This approach ensures both efficiency in production and flexibility in application. Professionals will collaborate in a test-improve-test circle, ColorFun will refine these formulations to ensure they work in real-world conditions. Students will be involved in the project, contributing to curriculum developments in biotechnology, chemistry, and materials science. Combining efforts, ColorFun lowers the barriers aiding fungal colorants to become a mainstream alternative to synthetic feedstocks. By making these colorants scientifically validated, industrially viable, and commercially adaptable, the project helps accelerate the transition to sustainable color solutions and circular economy.
The textile industry is one of the largest environmental polluters, primarily due to its water-intensive dyeing processes and the waste-water release of synthetic colorants which are harmful to the environment and human health. While there are efforts to improve sustainability, few solutions address both water usage and the need for renewable alternatives to synthetic dyes. FuntureTex presents an innovative solution by implementing fungal-based colorants with the doping dyeing process. Fungal colorants are renewable and biodegradable, providing a sustainable alternative to synthetic dyes. The doping process is a water-saving technique where colorants are integrated directly into textile fibers during their production, eliminating the need for traditional water-dependent dyeing steps. This method reduces water use by up to 90% compared to conventional dyeing, as no water is required to rinse or fix the dye. Til date, although efforts have been made on incorporating biobased substances in dope dyeing, the use of natural, especially fungal colorants, has not yet been done. In this project, the consortium will combine forces to improve dope dyeing by using fungal colorants. Key technologies including fermentation and green chemistry will be applied throughout the project. Firstly, the colorants will be produced via fermentation and treated with sustainable downstream processing methods. Afterwards, the colorants will be integrated into polymers and yarned as textile materials by using the dope dyeing method. The project results will be disseminated to professional fields to gain insights in business potentials. Students will be involved through the project to broaden the impact. The FuntureTex consortium is made by experts in areas of fungal colorants, dope dyeing and business outlook, ensuring a proper execution of the project. In line with the EU’s goal of circular and sustainable economy, the research result will bring a potential dual solution to the environmental challenges of textile industries.
