This paper presents a proof of concept for monitoring masonry structures using two different types of markers which are not easily noticeable by human eye but exhibit high reflection when subjected to NIR (near-infrared) wavelength of light. The first type is a retroreflective marker covered by a special tape that is opaque in visible light but translucent in NIR, while the second marker is a paint produced from infrared reflective pigments. The reflection of these markers is captured by a special camera-flash combination and processed using image processing algorithms. A series of experiments were conducted to verify their potential to monitor crack development. It is shown that the difference between the actual crack width and the measured was satisfactorily small. Besides that, the painted markers perform better than the tape markers both in terms of accuracy and precision, while their accuracy could be in the range of 0.05 mm which verifies its potential to be used for measuring cracks in masonry walls or plastered and painted masonry surfaces. The proposed method can be particularly useful for heritage structures, and especially for acute problems like foundation settlement. Another advantage of the method is that it has been designed to be used by non-technical people, so that citizen involvement is also possible in collecting data from the field.
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Knowledge of the time of deposition is pivotal in forensic investigations. Recent studies show that changes in intrinsic fluorescence over time can be used to estimate the age of body fluids. These changes have been attributed to oxidative modifications caused by protein–lipid interactions. This pilot study aims to explore the impact of these modifications on body fluid fluorescence, enhancing the protein–lipid model system for age estimation. Lipid and protein oxidation markers, including protein carbonyls, dityrosine, advanced glycation end-products (AGEs), malondialdehyde (MDA), and 4-hydroxynonenal (HNE), were studied in aging semen, urine, and saliva over 21 days. Surface plasmon resonance imaging (SPRi), enzyme-linked immunosorbent assay (ELISA), and fluorescence spectroscopy were applied. Successful detection of AGE, dityrosine, MDA, and HNE occurred in semen and saliva via SPRi, while only dityrosine was detected in urine. Protein carbonyls were measured in all body fluids, but only in saliva was a significant increase observed over time. Additionally, protein fluorescence loss and fluorescent oxidation product formation were assessed, showing significant decreases in semen and saliva, but not in urine. Although optimization is needed for accurate quantification, this study reveals detectable markers for protein and lipid oxidation in aging body fluids, warranting further investigation.
MULTIFILE
Fatigued pilots are prone to experience cognitive disorders that degrade their performance and adherence to high safety standards. In light of the current challenging context in aviation, we report the early phase of our ongoing project on the re-evaluation of human factors research for flight crew. Our motivation stems from the need for aviation organisations to develop decision support systems for operational aviation settings, able to feed-in in the organisations’ fatigue risk management efforts. Key criteria to this end are the need for the least possible intrusiveness and the added information value for a safety system. Departing from the problems in compliance-focused fatigue risk management and the intrusive nature of clinical studies, we report a neuroscientific methodology able to yield markers that can be easily integrated in a decision support system at the operational level. Reporting the preliminary phase of our live project, we evaluate the tools suitable for the development of a system that tracks subtle pilot states, such as drowsiness and micro-sleep episodes.
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