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Transient repression of the synthesis of OmpF and aspartate transcarbamoylase in Escherichia K12 as a response to pollutant stress

The synthesis of total cellular proteins in Escherichia coli K12 was studied in batch culture following exposure of cells to low concentrations of monochlorophenol, pentachlorophenol and cadmium chloride. Changes in protein patterns were identified after pulse-chase labelling of proteins with [35S]methionine and subsequent two-dimensional gel electrophoresis (2D-PAGE). We demonstrated that besides the induction of some stress proteins, also a transient decrease in the rate of synthesis of other proteins occurred. Two of these proteins were identified as OmpF and aspartate transcarbamoylase (ATCase). Their transient repression appeared to be a general response to stress elicited by different pollutants and may therefore be used as a general and sensitive early warning system for pollutant stress.

Asthma and Air Pollution: Connecting the Dots

Two key air pollutants that affect asthma are ozone and particle pollution. Studies show a direct relationship between the number of deaths and hospitalizations for asthma and increases of particulate matter in the air, including dust, soot, fly ash, diesel exhaust particles, smoke, and sulfate aerosols. Cars are found to be a primary contributor to this problem. However, patient awareness of the link is limited. This chapter begins with a general discussion of vehicular dependency or ‘car culture’, and then focuses on the discussion of the effects of air pollution on asthma in the Netherlands. I argue that international organizations and patient organizations have not tended to put pressure on air-control, pollution-control or environmental standards agencies, or the actual polluters. While changes in air quality and the release of greenhouse gases are tied to practices like the massive corporate support for the ongoing use of motor vehicles and the increased prominence of ‘car culture’ globally, patient organizations seem more focused on treating the symptoms rather than addressing the ultimate causes of the disease. Consequently, I argue that to fully address the issue of asthma the international health organizations as well as national health ministries, patient organizations, and the general public must recognize the direct link between vehicular dependency and asthma. The chapter concludes with a recommendation for raising environmental health awareness by explicitly linking the vehicular dependency to the state of poor respiratory health. Strategic policy in the Netherlands then should explicitly link the present pattern of auto mobility to public health. https://onlinelibrary.wiley.com/doi/book/10.1002/9781118786949 LinkedIn: https://www.linkedin.com/in/helenkopnina/

In situ mapping of pollutants in Sustainable Urban Drainage Systems, a new methodology approach and preliminary results from the Netherlands

Results of national In situ mapping of Potential Toxic Elements in green infrastructure

Stormwater runoff can contain high amounts of Potential Toxic Elements (PTE) as heavy metals. PTE can have negative and direct impact on the quality of surface waters and groundwater. The European Water Framework Directive (WFD) demands enhanced protection of the aquatic environment. As a consequence, the WFD requires municipalities and water authorities to address the emissions from drainage systems adequately and to take action when these emissions affect the quality of receiving waters together with mitigating the quantity challenges in a changing climate (floodings and drought). NBS is the most widely used method for storing stormwater and infiltrating in the Netherlands. However, there is still too little knowledge about the long-term functioning of the soil of these facilities. The research results are of great importance for all stakeholders in (inter)national cities that are involved in climate adaptation. Applying Nature-Based Solutions (NBS), Sustainable Urban Drainage Systems (SuDS) or Water Sensitive Urban Design (WSUD) are known to improve the water quality in the urban water cycle. The efficiency of NBS, such as the capability of bio swales to trap PTE, highly depends on the dimensions of the facility and on its implementation in the field [Woods Ballard, B et al, 2015]. For the determination of the removal efficiency of NBS information about stormwater quality and characteristics is essential. Acquiring the following information is strongly advised [Boogaard et al. 2014]:1. stormwater quality levels (method: stormwater quality database);2. location of NBS (method: mapping NBS in international database);3. behaviour of pollutants (method: cost effective mapping pollutants in the field). Stormwater quality contains pollutants as heavy metal in higher concentrations than water quality standards dictate. Over 500 locations with bio swales are mapped in the Netherlands which is a fraction of stormwater infiltration locations implemented in 20 years’ time. Monitoring of all these NBS would acquire high capacity and budget from the Dutch resources. This quick scan XRF mapping methodology of topsoil will indicate if the topsoil is polluted and whether the concentrations exceed national or international standards. This was only the case in one of the youngest pilots in Utrecht indicating that there are multiple factors other than age (traffic intensity, use of materials, storage volume, maintenance, run off quality, etc.). Several locations show unacceptable levels, above the national thresholds for pollutants where further research on the prediction of these levels in relation to multiple factors will be the subject of future research.The results of study are shared in 2 national workshops and valued as of great importance for all stakeholders in (inter)national cities that are involved in implementation of NBS for climate adaptation. The Dutch research results will be used to update (inter-)national guidelines for design, construction and maintenance of infiltration facilities this year. Stormwater managers are strongly advised to use this quick scan method within the first 10 years after implementation of swales to map possible pollution of the top soil and prevent pollution to spread to the groundwater in urban areas.

Stormwater quality characteristics in (Dutch) urban areas and performance of settlement basins

Stormwaters, flowing into storm sewers, are known to significantly increase the annual pollutant loads entering urban receiving waters and this results in significant degradation of the receiving water quality. Knowledge of the characteristics of stormwater pollution enables urban planners to incorporate the most appropriate stormwater management strategies to mitigate the effects of stormwater pollution on downstream receiving waters. This requires detailed information on stormwater quality, such as pollutant types, sediment particle size distributions, and how soluble pollutants and heavy metals attach themselves to sediment particles. This study monitored stormwater pollution levels at over 150 locations throughout the Netherlands. The monitoring has been ongoing for nearly 15 years and a total of 7,652 individual events have been monitored to date. This makes the database the largest stormwater quality database in Europe. The study compared the results to those presented in contemporary international stormwater quality research literature. The study found that the pollution levels at many of the Dutch test sites did not meet the requirements of the European Water Framework Directive (WFD) and Dutch Water Quality Standards. Results of the study are presented and recommendations are made on how to improve water quality with the implementation of Sustainable Urban Drainage Systems (SUDS) devices.

Investigation into the long-term stormwater pollution removal efficiency of bioretention systems

In recent years, there has been a steady increase in the number of bioretention systems installed worldwide. However, there has only been limited research on the long-term effectiveness of these sustainable urban drainage system devices. This paper presents the results of a series of controlled field experiments investigating the pollutant removal efficiency of three bio-filtration system that have been in service for over five years in the Sunshine Coast in Australia. The results of this study suggest that the long-term pollution removal performance of these systems may not be as effective as previously thought and further research is needed.