Background There currently is no field test available for measuring maximal exercise capacity in people with stroke. Objective To determine the feasibility, reproducibility and validity of the Shuttle Test (ST) to measure exercise capacity in people with stroke. Design Longitudinal study design. Setting Rehabilitation department, day care centres from a nursing home and private practices specialized in neuro rehabilitation. Subjects People with subacute or chronic stroke. Interventions A standardized protocol was used to determine feasibility, reproducibility and validity of the 10-meter Shuttle Test (10mST). Main measures Number of shuttles completed, 1stVentilatory Threshold (1stVT). Results The associations of the number of shuttles completed and cardiopulmonary capacity as measured with a portable gas analyser were r > 0.7, confirming good convergent validity in subacute and chronic people with stroke. Criterion validity, however, indicates it is not a valid test for measuring maximal cardiopulmonary capacity (VO2max). Only 60% of participants were able to reach the 1stVT. Higher cardiopulmonary capacity and a higher total score of the lower extremity Motricity Index contributed significantly to a higher number of shuttles walked (p = 0.001). Conclusions The Shuttle Test may be a safe and useful exercise test for people after stroke, but may not be appropriate for use with people who walk slower than 2 km/h or 0.56 m/s.
Characteristics of the physical childcare environment are associated with children’s sedentary behavior (SB) and physical activity (PA) levels. This study examines whether these associations are moderated by child characteristics. A total of 152 1- to 3-year-old children from 22 Dutch childcare centers participated in the study. Trained research assistants observed the physical childcare environment, using the Environment and Policy Assessment Observation (EPAO) protocol. Child characteristics (age, gender, temperament and weight status) were assessed using parental questionnaires. Child SB and PA was assessed using Actigraph GT3X+ accelerometers. Linear regression analyses including interaction terms were used to examine moderation of associations between the childcare environment and child SB and PA. Natural elements and portable outdoor equipment were associated with less SB and more PA. In addition, older children, boys and heavier children were less sedentary and more active, while more use of childcare and an anxious temperament were associated with more SB. There were various interactions between environmental factors and child characteristics. Specific physical elements (e.g., natural elements) were especially beneficial for vulnerable children (i.e., anxious, overactive, depressive/withdrawn, overweight). The current study shows the importance of the physical childcare environment in lowering SB and promoting PA in very young children in general, and vulnerable children specifically. Moderation by child characteristics shows the urgency of shaping childcare centers that promote PA in all children, increasing equity in PA promotion in childcare.
Plastic waste is one of the largest environmental problems in the 21st century. By 2050, up to 12,000 Mt of plastic waste is estimated to be in landfills or in the natural environment. Biochemical recycling by using modified microbial enzymes have shown potentials in the back-to-monomer (BTM) recycling of polyethylene terephthalate by breaking down the polymers into re-usable monomers. These enzymes can be produced via fungal species. In order to make this biochemical BTM process viable a process integrated enzyme production is key in increasing the efficiency and reducing the cost of enzymes. For this a molecular monitoring method, such as RNA-seq (RNA-sequencing), is needed. RNA-seq can achieve a snapshot on enzyme producing process inside of the cell by semi-quantitatively measuring the volume of enzyme encoding RNAs. This information can bring hints on fungal strain improvement by promoting the desired enzymes. It also helps to instantly monitor the BTM production outcomes. However, conventional RNA-seq platforms can only be performed via service providers or startup investments reaching 2 million euros. Each round of analysis could take as long as 6 weeks turnaround time. Furthermore, the method creates huge amount of complicated datasets, only by expert skills and specialized high performance computing the data can be sorted in a comprehensive manner. To solve these problems, in this project, by combining the expertise on plastic end-of-life control, fungal enzyme production, molecular monitoring and Bioinformatics from both the UAS and SME sides, we aim to implement a novel RNA-seq based system to monitor the in-process enzyme production for plastic degradation. We will optimize the existing portable RNA-seq prototype machinery for semi-real time monitoring of the BTM recycling process. The downstream data will be handled by a tailored analysis pipeline designed with expert knowledge via an user-friendly interface.
