The quantification and identification of new plasmid-acquiring bacteria in representative mating conditions is critical to characterize the risk of horizontal gene transfer in the environment. This study aimed to quantify conjugation events resulting from manure application to soils and identify the transconjugants resulting from these events. Conjugation was quantified at multiple time points by plating and flow cytometry, and the transconjugants were recovered by fluorescence-activated cell sorting and identified by 16S rRNA sequencing. Overall, transconjugants were only observed within the first 4 days after manure application and at values close to the detection limits of this experimental system (1.00–2.49 log CFU/g of manured soil, ranging between 10–5 and 10–4 transconjugants-to-donor ratios). In the pool of recovered transconjugants, we found amplicon sequence variants (ASVs) of genera whose origin was traced to soils (Bacillus and Nocardioides) and manure (Comamonas and Rahnella). This work showed that gene transfer from fecal to soil bacteria occurred despite the less-than-optimal conditions faced by manure bacteria when transferred to soils, but these events were rare, mainly happened shortly after manure application, and the plasmid did not colonize the soil community. This study provides important information to determine the risks of AMR spread via manure application.
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Permanent grassland soils can act as a sink for carbon and may therefore positively contribute to climate change mitigation and adaptation. We compared young (5–15 years since latest grassland renewal) with old (>20 years since latest grassland renewal) permanent grassland soils in terms of carbon stock, carbon sequestration, drought tolerance and flood resistance. The research was carried out on marine clay soil at 10 dairy farms with young and old permanent grassland. As hypothesized, the carbon stock was larger in old grassland (62 Mg C ha−1) topsoil (0–10 cm) than in young grassland topsoil (51 Mg C ha−1). The carbon sequestration rate was greater in young (on average 3.0 Mg C ha−1 year−1) compared with old grassland (1.6 Mg C ha−1 year−1) and determined by initial carbon stock. Regarding potential drought tolerance, we found larger soil moisture and soil organic matter (SOM) contents in old compared with young grassland topsoils. As hypothesized, the old grassland soils were more resistant to heavy rainfall as measured by water infiltration rate and macroporosity (at 20 cm depth) in comparison with the young grassland soils. In contrast to our hypothesis we did not find a difference in rooting between young and old permanent grassland, probably due to large variability in root biomass and root tip density. We conclude that old grasslands at dairy farms on clay soil can contribute more to the ecosystem services climate change mitigation and climate change adaptation than young grasslands. This study shows that under real farm conditions on a clay topsoil, carbon stock increases with grassland age and even after 30 years carbon saturation has not been reached. Further study is warranted to determine by how much extending grassland age can contribute to climate change mitigation and adaptation.
MULTIFILE
''Heritage buildings are often subjected to loading conditions that they were not exposed to in their earlier life span. Induced earthquakes in non-seismic regions caused by energy exploitation activities, or strains in the ground that are caused by the climate changes, are new phenomena that alter the usual loading situations for historical buildings.In this paper, monitoring results of a historical building in Groningen (Netherlands) in case of induced seismicity as well as climate change effects has been presented. Long-term monitoring results, detected cracks and relevance of the monitoring data are discussed. In the special case of Groningen, weak and agricultural soil properties dominate the structural response in the region. The gas extraction activities caused a soil subsidence in the giant Groningen Gas Field, resulting decameters of settlement in the entire area, thus an increase of the ground water level in respect to the ground surface. This is the reason why the heritage structures in the region are more vulnerable to soil-water-foundation interactions caused by climate change as compared to the time these heritage structures were constructed. The ground water monitoring as well as the interaction of soil movements with the structural response become important. The study presented here suggests ways on how to effectively monitor historical structures subjected to induced seismicity as well as harsh climate effects at the same time.It was shown here that the newly developed cracks on the structure were detected in a very narrow time window, coinciding with extreme drought and a small induced earthquake at the same time. One explanation provided here is that the soil parameters, such as shrinking of water-sensitive soil layers, in combination with small earthquakes, may cause settlements. The soil effects may superimpose with the earthquake effects eventually causing small cracks and damage. The effects of the climate change on historical buildings is rather serious, and structures on similar soil conditions around the world would need detailed monitoring of not only the structure itself but also the soil-foundation and ground water conditions.''
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Climate change is increasing the challenges for water management worldwide. Extreme weather conditions, such as droughts and heavy rainfall, are increasingly limiting the availability of water, especially for agriculture. Nature-Based Solutions (NBS) offer potential solutions. They help to collect and infiltrate rainwater and thus play an important role in climate adaptation.Green infrastructure, such as rain gardens (sunken plant beds) and wadis (sunken grass fields for temporary storage of rainwater), help to restore the urban water balance. They reduce rainwater runoff, stabilize groundwater levels and solve problems with soil moisture and temperature. Despite these advantages, there is still much ignorance in practice about the possibilities of NBS. To remedy this, freely accessible knowledge modules are being developed that can help governments and future employees to better understand the application of these solutions. This research, called GINA (Green Infrastructure in Urban Areas), aims to create more sustainable and climate-resilient cities by developing and sharing knowledge about NBS, and supports local governments and students in effectively deploying these green infrastructures.
This project has received funding from the Bio-based Industries Joint Undertaking under the European Union's Horizon 2020 research and innovation programme under grant agreement No 720726LIBBIO is a European research project on Andes Lupin (Lupinus mutabilis, tarwi) cropping in marginal lands for enhanced bio economy. Lupin has the ability to fix nitrogen, mobilise soil phosphate and has low nutritional requirements for cultivation. Varieties will be chosen that give high yield of green silage or high yield of seeds which contain more than 20% oil, more than 40% protein and the remaining materials are carbohydrates, mainly oligosaccharides characterized as “prebiotics”. Andes lupin will be grown as a summer crop in N-central Europe and as winter crop in Mediterranean conditions. Pre-industrial processing is developed and optimized for the lupin, properties of the different fractions analysed, their advantage for different industrial use evaluated, and a few products developed as an example. Social and environmental impact will be evaluated as well as techno-economic viability and effect on farm and biorefinery income.This project has received funding from the Bio-based Industries Joint Undertaking under the European Union's Horizon 2020 research and innovation programme under grant agreement No 720726
The project virtually breaks down a large timber structure into pieces and simultaneously simulates and tests each piece in a different laboratory or facility. In this way, unique aspects of each facility can be used at the same time. The experiments take place in a synchronized way, which is a difficult task considering 4 countries (UK, Canada, Greece and the Netherlands) will work at the same time for testing one hypothetical timber structure. Geographically distributed hybrid testing blue sky research, timber structure testing including soil-structure-interactionHYSTERESIS project aims to use geographically distributed hybrid testing for providing experimental evidence for energy dissipation and SSI response of buildings composed of mass timber and CLT panels. The project outcomes will give a boost to the efforts of building multi-story timber structures in areas with wind and/or earthquake loading conditions. The particularities of the problem in hand and the need for testing in large scale while taking into account the SSI, dictate using a novel hybrid testing approach.