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Assessing the utility of frequency tagging for tracking memory-based reactivation of word representations

Reinstatement of memory-related neural activity measured with high temporal precision potentially provides a useful index for real-time monitoring of the timing of activation of memory content during cognitive processing. The utility of such an index extends to any situation where one is interested in the (relative) timing of activation of different sources of information in memory, a paradigm case of which is tracking lexical activation during language processing. Essential for this approach is that memory reinstatement effects are robust, so that their absence (in the average) definitively indicates that no lexical activation is present. We used electroencephalography to test the robustness of a reported subsequent memory finding involving reinstatement of frequency-specific entrained oscillatory brain activity during subsequent recognition. Participants learned lists of words presented on a background flickering at either 6 or 15 Hz to entrain a steady-state brain response. Target words subsequently presented on a non-flickering background that were correctly identified as previously seen exhibited reinstatement effects at both entrainment frequencies. Reliability of these statistical inferences was however critically dependent on the approach used for multiple comparisons correction. We conclude that effects are not robust enough to be used as a reliable index of lexical activation during language processing.

Cluttering identified: differential diagnostics between cluttering, stuttering and speech impairment related to learning difficulties

Oxidative Modifications of Proteins and Lipids of Dried Semen, Urine, and Saliva Stains as a Function of Age in Forensic Context

Knowledge of the time of deposition is pivotal in forensic investigations. Recent studies show that changes in intrinsic fluorescence over time can be used to estimate the age of body fluids. These changes have been attributed to oxidative modifications caused by protein–lipid interactions. This pilot study aims to explore the impact of these modifications on body fluid fluorescence, enhancing the protein–lipid model system for age estimation. Lipid and protein oxidation markers, including protein carbonyls, dityrosine, advanced glycation end-products (AGEs), malondialdehyde (MDA), and 4-hydroxynonenal (HNE), were studied in aging semen, urine, and saliva over 21 days. Surface plasmon resonance imaging (SPRi), enzyme-linked immunosorbent assay (ELISA), and fluorescence spectroscopy were applied. Successful detection of AGE, dityrosine, MDA, and HNE occurred in semen and saliva via SPRi, while only dityrosine was detected in urine. Protein carbonyls were measured in all body fluids, but only in saliva was a significant increase observed over time. Additionally, protein fluorescence loss and fluorescent oxidation product formation were assessed, showing significant decreases in semen and saliva, but not in urine. Although optimization is needed for accurate quantification, this study reveals detectable markers for protein and lipid oxidation in aging body fluids, warranting further investigation.

Calibration of a CubeSat spectroradiometer with a narrow-band widely tunable radiance source

We have developed an SI-traceable narrow-band tunable radiance source based on an optical parametric oscillator (OPO) and an integrating sphere for the calibration of spectroradiometers. The source is calibrated with a reference detector over the ultraviolet/visible spectral range with an uncertainty of <1%. As a case study, a CubeSat spectroradiometer has been calibrated for radiance over its operating range from 370 nm to 480 nm. To validate the results, the instrument has also been calibrated with a traditional setup based on a diffuser and an FEL lamp. Both routes show good agreement within the combined measurement uncertainty. The OPO-based approach could be an interesting alternative to the traditional method, not only because of reduced measurement uncertainty, but also because it directly allows for wavelength calibration and characterization of the instrumental spectral response function and stray light effects, which could reduce calibration time and cost.

Performance Characterization of an Illumination-Based Low-Cost Multispectral Camera

Spectral imaging has many applications, from methane detection using satellites to disease detection on crops. However, spectral cameras remain a costly solution ranging from 10 thousand to 100 thousand euros for the hardware alone. Here, we present a low-cost multispectral camera (LC-MSC) with 64 LEDs in eight different colors and a monochrome camera with a hardware cost of 340 euros. Our prototype reproduces spectra accurately when compared to a reference spectrometer to within the spectral width of the LEDs used and the ±1σ variation over the surface of ceramic reference tiles. The mean absolute difference in reflectance is an overestimate of 0.03 for the LC-MSC as compared to a spectrometer, due to the spectral shape of the tiles. In environmental light levels of 0.5 W m−2 (bright artificial indoor lighting) our approach shows an increase in noise, but still faithfully reproduces discrete reflectance spectra over 400 nm–1000 nm. Our approach is limited in its application by LED bandwidth and availability of specific LED wavelengths. However, unlike with conventional spectral cameras, the pixel pitch of the camera itself is not limited, providing higher image resolution than typical high-end multi- and hyperspectral cameras. For sample conditions where LED illumination bands provide suitable spectral information, our LC-MSC is an interesting low-cost alternative approach to spectral imaging.

Narrow-band and widely tunable radiance source for the calibration of spectral imaging instruments

Calibration of spectral imaging instruments is a prerequisite for many applications, in particular in the field of Earth observation. In this contribution we will present a novel traceability route to celebrate spectral imaging instruments, based on tunable radiance source that is referenced to a primary detector standard.