Dark homogenous fungal-based layers called biofinishes and vegetable oils are keyingredients of an innovative wood protecting system. The aim of this study was todetermine which of the vegetable oils that have been used to generate biofinishes onwood will provide carbon and energy for the biofinish-inhabiting fungus Aureobasidiummelanogenum, and to determine the effect of the oil type and the amount of oil on thecell yield. Aureobasidium melanogenum was cultivated in shake flasks with differenttypes and amounts of carbon-based nutrients. Oil-related total cell and colony-formingunit growth were demonstrated in suspensions with initially 1% raw linseed,stand linseed, and olive oil. Oil-related cell growth was also demonstrated with rawlinseed oil, using an initial amount of 0.02% and an oil addition during cultivation. Nilered staining showed the accumulation of fatty acids inside cells grown in the presenceof oil. In conclusion, each tested vegetable oil was used as carbon and energysource by A. melanogenum. The results indicated that stand linseed oil provides lesscarbon and energy than olive and raw linseed oil. This research is a fundamental stepin unraveling the effects of vegetable oils on biofinish formation.
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Vegetables have low taste intensities, which might contribute to low acceptance. The aim of this study was to investigate the effect of taste (sweetness, sourness, bitterness, umami, and saltiness) and fattiness enhancement on consumer acceptance of cucumber and green capsicum purees. Three concentrations of sugar, citric acid, caffeine, mono-sodium glutamate, NaCl, and sunflower oil were added to pureed cucumber and green capsicum. Subjects (n = 66,35.6 ± 17.7 y) rated taste and fattiness intensity. Different subjects (n = 100, 33.2 ± 16.5 years) evaluated acceptance of all pureed vegetables. Taste intensities of vegetable purees were significantly different (P < 0.05) between the three tastant concentrations except for umami in both vegetable purees, sourness in green capsicum puree, and fattiness in cucumber puree. Only enhancement of sweetness significantly (P < 0.05) increased acceptance of both vegetable purees compared to unmodified purees. In cucumber purees, relatively small amounts of added sucrose (2%) increased acceptance already significantly, whereas in green capsicum acceptance increased significantly only with addition of 5% sucrose. Enhancement of other taste modalities did not significantly increase acceptance of both vegetable purees. Enhancing saltiness and bitterness significantly decreased acceptance of both vegetable purees. We conclude that the effect of taste enhancement on acceptance of vegetable purees differs between tastants and depends on tastant concentration and vegetable type. With the exception of sweetness, taste enhancement of taste modalities such as sourness, bitterness, umami, and saltiness was insufficient to increase acceptance of vegetable purees. We suggest that more complex taste, flavor, or texture modifications are required to enhance acceptance of vegetables.
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In this article, we assess the potential of alternative land use systems using non-drainage peatland species which could eventually phase out or partly replace oil palm plantations on undrainable peatlands. We have used the ecosystem services approach to analyse what scenarios using drainage-free peatland species could be suitable alternatives for oil palm cultivation on peat and how these scenarios compare to oil palm plantations in terms of selected ecosystem services. Our results indicate that alternative paludiculture systems will provide more direct and indirect ecosystem services than oil palm plantations on peat. We also found that stakeholders were aware of issues with growing oil palm on peat, and that there was a general intention for sustainable use of peatlands amongst several groups of stakeholders. Replacing oil palm with alternative systems such as paludiculture in Malaysia is not yet realistic. The most important impediments are a lack of knowledge on potential of non-drainage peatland species and its associated value chains, as well as the technical difficulty for smallholders to implement such a system. We recommend starting experimental plantings with paludiculture systems to further test species performance, life cycle analysis, growth, intercropping limitations and possibilities, yields and improvements in the value chain.
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Active antifungal packaging is a technological solution for reducing the postharvest losses of fruits and vegetables associated with phytopathogens. Anthracnose (Colletotrichum gloeosporioides) is the principal fungus that causes post-harvest avocado fruit decay. In this study, antifungal sachets filled with oregano oil-starch capsules were prepared, and their active effects were demonstrated on Hass avocado fruits. Oregano oil (31 % of carvacrol) was encapsulated with corn starch by spray drying. Tyvek sachets (4 × 4 cm) filled with 80 (T1) and 160 mg (T2) of oregano oil-starch capsules (99.35 ± 1.86 mg g − 1) were fabricated. The antifungal effects of the sachets were tested in vitro and in vivo using a humidity chamber (90–95 % relative humidity (RH)) on fruits inoculated with anthracnose. The results showed that T1 and T2 inhibited 75.21 ± 2.81 and 100 % in vitro growth of anthracnose at 25 °C for 12 days. Furthermore, Hass avocado fruits stored in a humidity chamber at 25 °C for 6 days showed that only T2 significantly (p < 0.05) reduced the area of lesion produced by artificial inoculation of Hass avocado fruits with anthracnose. On average, the lesion area in the Hass avocado fruits treated with T2 was 13.94 % smaller than that in the control fruit.
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Smallholders are a substantial part of the oil palm sector and thus key to achieve more sustainable production. However, so far their yields remain below potential. The Roundtable on Sustainable Oil Palm (RSPO) aims to include smallholders in sustainability certification to strengthen rural livelihoods and reduce negative environmental impacts. This study aims to determine if and how certified smallholders perform differently from their non-certified counterparts in terms of management practices and yields, and to what extend this is related to RSPO certification.
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Fish and vegetable protein sources are relatively underutilized for human consumption in comparison to meat, dairy and egg protein sources. Only part of the available fish proteins is used: fish is to small for human consumption and fish has a high proportion of by-products, up to 50% of fish weight is not used. This project aims to develop products and processes for creating healthy high valued consumer products based upon vegetable proteins and fish/crustacean proteins from by-products or from neglected fish. Three innovative processes are developed:1) Iso-electric solubilization and precipitation of fish/crustacean proteins from by-products,2) Networked vegetable/fish protein textures based upon low moisture extrusion processes3) Fibrous vegetable/fish protein textures produced with high moisture extrusion processes.Two innovative processes are applied:1) Food products with water-oil-water emulsions with isolated fish proteins2) Food products with sous-vide prepared fish fillets in semi industrial context.Different consumer product prototypes will be developed like fish nuggets, fish flakes and fish crackers.The Nuprotex project created successfully two new processes. Hanzehogeschool developed the process for fish protein isolation based upon iso electric solubilization and precipitation. With this process it was possible to recover about 15% weight of additional proteins from fish by-products. Please be aware that the yield of fish fillets from the fish is only about 30% of fish weight. So this is an important increase in food grade proteins! These Isolated Fish Proteins are successfully converted into several consumer prototype products like multiple emulsions for savory liquid products and fish cake/cracker applications. A sous-vide cooking process for fish fillets was developed with respect to microbial safety. It was shown that a microbial safe route could be developed, however further research is necessary to confirm these preliminary results.DIL has developed successfully an high moisture extrusion process for isolated fish proteins, grinded fish by products and vegetable proteins. This semi-finished product is successfully applied by for developing deep fried fish nuggets and fish burgers. DIL produced fish pellets which are suitable for applications as fish feed as is demonstrated in actual trials. Further research must demonstrate the quality of the feed product in actual growth experiments with fish.This project has clearly demonstrated that it is possible to produce with fish by-products added value consumer products. A possible increase of food-grade fish protein of about 15% on fresh weight base of processed fish is possible.
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Docentonderzoeker Rob van Diepenbeek was een van de sprekers op de Intelligent Food processing & packaging Virtual Summit, een online platform waar foodprofessionals kunnen netwerken, ideeën en kennis uitwisselen en van elkaar leren waar het gaat om toekomst van ons voedsel. Rob ging onder meer in op de nauwe samenwerking van HAS Hogeschool met Food Tech Brainport in Helmond, een belangrijke experimenteerruimte als het gaat om het terugdringen van voedselverspilling en bij- en reststromen te verwaarden door middel van milde conservering en milde scheidingstechnieken. Veelbelovende productapplicatie-kansen en energiebesparingen kwamen aan bod.
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COTOPAXI is an ANDEAN lupin which has obtained Plant Breeder Rights by Vandinter Semo BV, the Netherlands. COTOPAXI is rich in protein (45%) and oils (20%). COTOPAXI will positively contribute to the plant-based protein transition in hte Netherlands and Europe.
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Bitterness has been suggested to be the main reason for the limited palatability of several vegetables. Vegetable acceptance has been associated with preparation method. However, the taste intensity of a variety of vegetables prepared by differentmethods has not been studied yet. The objective of this study is to assess the intensity of the five basic tastes and fattiness of ten vegetables commonly consumed in the Netherlands prepared by different methods using the modified Spectrum method. Intensities of sweetness, sourness, bitterness, umami, saltiness and fattiness were assessed for ten vegetables (cauliflower, broccoli, leek, carrot, onion, red bell pepper, French beans, tomato, cucumber and iceberg lettuce) by a panel (n = 9) trained in a modified Spectrum method. Each vegetable was assessed prepared by different methods (raw, cooked, mashed and as a cold pressed juice). Spectrum based reference solutions were available with fixed reference points at 13.3 mm (R1), 33.3mm(R2) and 66.7mm(R3) for each tastemodality on a 100mmline scale. For saltiness, R1 and R3 differed (16.7 mm and 56.7 mm). Mean intensities of all taste modalities and fattiness for all vegetables were mostly below R1 (13.3 mm). Significant differences (p b 0.05) within vegetables between preparation methods were found. Sweetness was the most intensive taste, followed by sourness, bitterness, fattiness, umami and saltiness.In conclusion, all ten vegetables prepared by different methods showed low mean intensities of all taste modalities and fattiness. Preparation method affected taste and fattiness intensity and the effect differed by vegetable type.
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Active sachets can be used to manage postharvest losses associated with phytopathogens in fruit and vegetables. Diseases associated with phytopathogens are the principal causes of avocado losses postharvest. This study was performed to develop antifungal active sachet-based oregano oil microencapsulated with starch/agave fructans that allows in vivo and in vitro control of phytopathogens associated with avocado decay. In addition, avocado-sachet interactions were studied. Oregano oil sachets inhibited 100% of the in vitro growth of Colletotrichum gloeosporioides, Colletotrichum acutatum, Diaporthe passiflorae, and Neoscytalidium hyalinum at 30 °C for 12 d The efficacy of the oregano oil sachets was confirmed on avocados inoculated with C. gloeosporioides. Active sachets reduced the injury area of anthracnose infection in avocado without negative effects on the color or firmness of the fruit, compared to untreated control. Treatment also caused significantly higher (p < 0.05) phenylalanine ammonia-lyase, chitinase, β-1,3-glucanase, catalase and peroxidase activities compared to the control fruit. In addition, antifungal sachets significantly enhanced the contents of total flavonoids and phenolic compounds in treated avocados.
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