Plasmid-mediated dissemination of antibiotic resistance among fecal Enterobacteriaceae in natural ecosystems may contribute to the persistence of antibiotic resistance genes in anthropogenically impacted environments. Plasmid transfer frequencies measured under laboratory conditions might lead to overestimation of plasmid transfer potential in natural ecosystems. This study assessed differences in the conjugative transfer of an IncP-1 (pKJK5) plasmid to three natural Escherichia coli strains carrying extended-spectrum beta-lactamases, by filter mating. Matings were performed under optimal laboratory conditions (rich LB medium and 37°C) and environmentally relevant temperatures (25, 15 and 9°C) or nutrient regimes mimicking environmental conditions and limitations (synthetic wastewater and soil extract). Under optimal nutrient conditions and temperature, two recipients yielded high transfer frequencies (5 × 10–1) while the conjugation frequency of the third strain was 1000-fold lower. Decreasing mating temperatures to psychrophilic ranges led to lower transfer frequencies, albeit all three strains conjugated under all the tested temperatures. Low nutritive media caused significant decreases in transconjugants (−3 logs for synthetic wastewater; −6 logs for soil extract), where only one of the strains was able to produce detectable transconjugants. Collectively, this study highlights that despite less-than-optimal conditions, fecal organisms may transfer plasmids in the environment, but the transfer of pKJK5 between microorganisms is limited mainly by low nutrient conditions.
MULTIFILE
The WATERMINING project aims to bring solutions to improve the circularity of water treatment and the resulting by-products of these processes. Achieving a deep understanding of the barriers potentially hindering the development of circular water solutions is crucial to design policies that enable the deployment of these techniques. To do this, the WATERMINING project organizes Communities of Practice (CoPs), where stakeholders from the WATERMINING case study projects analysed these market barriers and proposal (policy) measures to clear these.CoPs in the case studies of Lampedusa in Italy and Almería in Spain focused on sea water desalination. The case studies of Faro-Olhão in Portugal, Larnaca in Cyprus and La Llagosta in Spain have been discussed by CoP stakeholders in terms of barriers in circular urban wastewater treatment. The CoP in the Netherlands focused on circular industrial waste water treatment at the Westlake plant at Rotterdam. The barriers defined by the stakeholders in the CoPs were discussed by the WATERMINING partners at the consortium meeting in Palermo (Italy, September 2022), and presented at the WATERMINING Market and Policy workshop in Brussels (Belgium, February 2023).Addressing the three above-mentioned categories of circular water solutions, common barriers identified across all WATERMINING’s case studies are the following. First, stakeholders report a lack of incentives to implement circular solutions, as mainstream linear practices are generally cheaper.This could be addressed by de-encouraging linear techniques by making the disposal of their byproducts (such as brine) more expensive. Another solution could be to provide added value to circular solutions through the monetization of their additional products and services. Subsidies can support in lowering production costs or prices of materials recovered from sea- and wastewater treatment to level the playing field with conventionaly derived material.Another commonly mentioned barrier is the difficulty to introduce products obtained from circular water treatment in the market, both because of a lack of public acceptance and legal constraints stemming from products being regarded as waste. Information campaigns and the revision of current regulatory frameworks to allow these products entering the market would expand the revenue sources from these techniques and improve the circularity of the system. Standardising the circular water treatment technologies in the market could support this, whereby best available techniques reference documents of the EU (BREFs) could be an effective instrument, especially when tapping into an ongoing BREF writing or updating process.Across the case studies and replication studies it has been mentioned that current legislation in case study countries exclude ‘watermined’ products from food and/or other applications. Criteria for endof-waste status of ‘watermined’ products, which would determine whether a product, such as Kaumera which is produced from urban wastewater treatment, is eligible as a fertiliser in agriculture, are usually determined at the level of the EU, but Member States could interpret these more stringently (Member State-level criteria cannot be weaker than the EU-level ones). In this respect it has been recommended to enhance knowledge exchange across Member States, e.g., by creating anEU-based unit (or competencies within an existing unit) to promote cooperation among EU Member States and regional authorities concerning the production, sale and use of products recovered from wastewater treatment.Another common perception stakeholders report is the widespread conservatism in the water sector. Water treatment actors traditionally have a focus on purifying water and supplying this to the market. Generating products from waste streams is often something that market actors are less familiar with. Among other solutions, the ‘Dutch model’ has been recommended as a way to create national centres for the development of knowledge and technology for water management, which would serve as an R&D accelerator.
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Climate change and urbanization will increase the frequency and magnitude of urban flooding and water quality problems in many regions of the world. In coastal and delta areas like The Netherlands and the Philippines, where urbanization is often high, there has been an increase in the adoption of sustainable urban drainage systems (SUDS). SUDS are installed around the world with the expectation to reduce urban flooding and reduce the pollution impact on receiving waters. Most cities in Asia are starting to implement SUDS as their strategy to make their cities sustainable and resilient.The combination of SUDS with appropriate wastewater treatment and management systems have the potential to be multifunctional in alleviating flood run-off, improving water quality, alleviating heat stress and as a source for reusing the stormwater and wastewater.Since the earliest SUDS are implemented in Europe decades ago it is advised to use the lessons learnt in this process. International knowledge exchange is promoted in projects as IWASTO where several organisations from the Philippines and The Netherlands join forces on a specific region as the Pateros riverin Manila with the aim to minimise the pollution impact on this receiving water. The first findings of this project related to storm water and wastewater management are presented in this paper. In this stage of the project high level support models that map the challenges in the city (such as flooding and heatstress) arevaluable tools for implementing cost effective sustainable drainage for improving water quality.
Due to the existing pressure for a more rational use of the water, many public managers and industries have to re-think/adapt their processes towards a more circular approach. Such pressure is even more critical in the Rio Doce region, Minas Gerais, due to the large environmental accident occurred in 2015. Cenibra (pulp mill) is an example of such industries due to the fact that it is situated in the river basin and that it has a water demanding process. The current proposal is meant as an academic and engineering study to propose possible solutions to decrease the total water consumption of the mill and, thus, decrease the total stress on the Rio Doce basin. The work will be divided in three working packages, namely: (i) evaluation (modelling) of the mill process and water balance (ii) application and operation of a pilot scale wastewater treatment plant (iii) analysis of the impacts caused by the improvement of the process. The second work package will also be conducted (in parallel) with a lab scale setup in The Netherlands to allow fast adjustments and broaden evaluation of the setup/process performance. The actions will focus on reducing the mill total water consumption in 20%.
Phosphorus is an essential element for life, whether in the agricultural sector or in the chemical industry to make products such as flame retardants and batteries. Almost all the phosphorus we use are mined from phosphate rocks. Since Europe scarcely has any mine, we therefore depend on imported phosphate, which poses a risk of supply. To that effect, Europe has listed phosphate as one of its main critical raw materials. This creates a need for the search for alternative sources of phosphate such as wastewater, since most of the phosphate we use end up in our wastewater. Additionally, the direct discharge of wastewater with high concentration of phosphorus (typically > 50 ppb phosphorus) creates a range of environmental problems such as eutrophication . In this context, the Dutch start-up company, SusPhos, created a process to produce biobased flame retardants using phosphorus recovered from municipal wastewater. Flame retardants are often used in textiles, furniture, electronics, construction materials, to mention a few. They are important for safety reasons since they can help prevent or spread fires. Currently, almost all the phosphate flame retardants in the market are obtained from phosphate rocks, but SusPhos is changing this paradigm by being the first company to produce phosphate flame retardants from waste. The process developed by SusPhos to upcycle phosphate-rich streams to high-quality flame retardant can be considered to be in the TRL 5. The company seeks to move further to a TRL 7 via building and operating a demo-scale plant in 2021/2022. BioFlame proposes a collaboration between a SME (SusPhos), a ZZP (Willem Schipper Consultancy) and HBO institute group (Water Technology, NHL Stenden) to expand the available expertise and generate the necessary infrastructure to tackle this transition challenge.
The textile industry faces a significant environmental challenge, annually generating 45 million tons of waste cotton textiles, of which 75% are incinerated or sent to landfills, causing environmental harm. Additionally, 67% of garments are made of plastic fibers, and when disposed of in landfills, 5% of them turn into microplastics that can end up on our plates. Chicfashic proposes an innovative biotech process to address these issues by recovering and recycling plastic fibers while transforming natural fibers into bio-based molecules. These molecules are then used as secondary raw materials to produce bio-based pigments for textiles. The project aims to optimize this process and test it on a larger scale with the assistance of HAN BioCentre. This initiative aligns with Dutch government and EU regulations mandating textile recycling by 2050. The technology used is patent pending and does not involve the use of toxic chemicals or the release of harmful wastewater or fumes, contributing to a shift towards a more circular and sustainable textile industry by reintegrating natural colorants into textile production.
