Despite decades of residence, Turkish-Dutch citizens, one of the largest immigrant groups in the Netherlands, continue to face significant disparities in health, social, and economic factors compared to native Dutch citizens. To better understand this persistent disparity, we examined the acculturation process of Turkish-Dutch citizens across three generations. Our study addressed two critical research gaps: (1) acculturation processes across three generations within a specific immigrant group, and (2) different acculturation domains across these generations. Data from 464 participants (232 Turkish-Dutch, 232 Dutch) show that acculturation varies significantly across generations (1st, 2nd, and 3rd) and domains (i.e., national identification, cultural values, language, and media use), with the second generation demonstrating the strongest resemblance to native Dutch citizens in most domains. These findings contribute to a nuanced understanding of acculturation processes and confirm the need for future research to consider generational differences and domain-specificity. The results have potential implications for policymakers and practitioners aiming to reduce disparities of Turkish-Dutch citizens with tailored policy and communication strategies.