The transition to a biobased economy necessitates utilizing renewable resources as a sustainable alternative to traditional fossil fuels. Bioconversion is a way to produce many green chemicals from renewables, e.g., biopolymers like PHAs. However, fermentation and bioconversion processes mostly rely on expensive, and highly refined pure substrates. The utilization of crude fractions from biorefineries, especially herbaceous lignocellulosic feedstocks, could significantly reduce costs. This presentation shows the microbial production of PHA from such a crude stream by a wild-type thermophilic bacterium Schlegelella thermodepolymerans [1]. Specifically, it uses crude xylose-rich fractions derived from a newly developed biorefinery process for grassy biomasses (the ALACEN process). This new stepwise mild flow-through biorefinery approach for grassy lignocellulosic biomass allows the production of various fractions: a fraction containing esterified aromatics, a monomeric xylose-rich stream, a glucose fraction, and a native-like lignin residue [2]. The crude xylose-rich fraction was free of fermentation-inhibiting compounds meaning that the bacterium S.thermodepolymerans could effectively use it for the production of one type of PHA, polyhydroxybutyrate. Almost 90% of the xylose in the refined wheat straw fraction was metabolized with simultaneous production of PHA, matching 90% of the PHA production per gram of sugars, comparable to PHA yields from commercially available xylose. In addition to xylose, S. thermodepolymerans converted oligosaccharides with a xylose backbone (xylans) into fermentable xylose, and subsequently utilized the xylose as a source for PHA production. Since the xylose-rich hydrolysates from the ALACEN process also contain some oligomeric xylose and minor hemicellulose-derived sugars, optimal valorization of the C5-fractions derived from the refinery process can be obtained using S. thermodepolymerans. This opens the way for further exploration of PHA production from C5-fractions out of a variety of herbaceous lignocellulosic biomasses using the ALACEN process combined with S. thermodepolymerans. Overall, the innovative utilization of renewable resources in fermentation technology, as shown herein, makes a solid contribution to the transition to a biobased economy.[1] W. Zhou, D.I. Colpa, H. Permentier, R.A. Offringa, L. Rohrbach, G.J.W. Euverink, J. Krooneman. Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans. Resources, Conservation and Recycling 194 (2023) 107006, ISSN 0921-3449, https://doi.org/10.1016/j.resconrec.2023.107006. [2] S. Bertran-Llorens, W.Zhou. M.A.Palazzo, D.I.Colpa, G.J.W.Euverink, J.Krooneman, P.J.Deuss. ALACEN: a holistic herbaceous biomass fractionation process attaining a xylose-rich stream for direct microbial conversion to bioplastics. Submitted 2023.
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The dynamic inflow effect describes the unsteady aerodynamic response to fast changes in rotor loading due to the inertia of the wake. Fast changes in turbine loading due to pitch actuation or rotor speed transients lead to load overshoots. The phenomenon is suspected to be also relevant for gust situations; however, this was never shown, and thus the actual load response is also unknown. The paper’s objectives are to prove and explain the dynamic inflow effect due to gusts, and compare and subsequently improve a typical dynamic inflow engineering model to the measurements. An active grid is used to impress a 1.8m diameter model turbine with rotor uniform gusts of the wind tunnel flow. The influence attributed to the dynamic inflow effect is isolated from the comparison of two experimental cases. Firstly, dynamic measurements of loads and radially resolved axial velocities in the rotor plane during a gust situation are performed. Secondly, corresponding quantities are linearly interpolated for the gust wind speed from lookup tables with steady operational points. Furthermore,simulations with a typical blade element momentum code and a higher-fidelity free-vortex wake model are performed. Both the experiment and higher-fidelity model show a dynamic inflow effect due to gusts in the loads and axial velocities. An amplification of induced velocities causes reduced load amplitudes. Consequently, fatigue loading would be lower. This amplification originates from wake inertia. It is influenced by the coherent gust pushed through the rotor like a turbulent box. The wake is superimposed on that coherent gust box, and thus the inertia of the wake and consequently also the flow in the rotor plane is affected. Contemporary dynamic inflow models inherently assume a constant wind velocity. They filter the induced velocity and thus cannot predict the observed amplification of the induced velocity. The commonly used Øye engineering model predicts increased gust load amplitudes and thus higher fatigue loads. With an extra filter term on the quasi-steady wind velocity, the qualitative behaviour observed experimentally and numerically can be caught. In conclusion, these new experimental findings on dynamic inflow due to gusts and improvements to the Øye model enable improvements in wind turbine design by less conservative fatigue loads.
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