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The synthesis of total cellular proteins in Escherichia coli K12 was studied in batch culture following exposure of cells to low concentrations of monochlorophenol, pentachlorophenol and cadmium chloride. Changes in protein patterns were identified after pulse-chase labelling of proteins with [35S]methionine and subsequent two-dimensional gel electrophoresis (2D-PAGE). We demonstrated that besides the induction of some stress proteins, also a transient decrease in the rate of synthesis of other proteins occurred. Two of these proteins were identified as OmpF and aspartate transcarbamoylase (ATCase). Their transient repression appeared to be a general response to stress elicited by different pollutants and may therefore be used as a general and sensitive early warning system for pollutant stress.
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Objective: The aim of this study was to obtain insight in specific elements influencing the use, non-use, satisfaction, and dissatisfaction of ankle foot orthoses (AFOs) and the presence of underexposed problems with respect to AFOs. Methods: A questionnaire was composed to obtain information from AFO users to investigate the variables associated with satisfaction and the relation between these variables. A specific feature of this study was the systematic analysis of the remarks made by the respondents about their AFO. Quantitative data analyses were used for analysing the satisfaction and qualitative analyses were used analysing the remarks of the respondents. A total of 211 users completed the questionnaire. Results: Our survey showed that 1 out of 15 AFOs were not used at all. About three quarters of the AFO users were satisfied and about one quarter was dissatisfied. Females and users living alone reported relatively high levels of dissatisfaction, especially in the field of dimensions, comfort, weight, safety and effectiveness. Dissatisfaction with respect to off-the-shelf AFOs for the item durability was higher than that for custom-made AFOs. In the delivery and maintenance process the items ‘maintenance’, ‘professionalism’ and ‘delivery follow-up’ were judged to be unsatisfactory. A large number of comments were made by the respondents to improve the device or process, mainly by the satisfied AFO users. These comments show that even satisfied users experience many problems and that a lot of problems of AFO users are ‘underexposed’. Conclusion: To improve user satisfaction, the user practice has to be identified as an important sub-process of the whole orthopaedic chain especially in the diagnosis and prescription, delivery tuning and maintenance, and evaluation phase.
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Increased intestinal permeability is linked to both intestinal as well as peripheralinflammatory disease. Contact between intestinal contents and the underlying immune system may account for excessive immune activation and local as well as systemic inflammation. Decreasing intestinal permeability using next-generation food products provides an attractive strategy to improve human health. However, to date, insight in biomarkers which reliably and reproducibly reflect intestinal permeability are lacking. Insight in these biomarkers provides a method to easily assess the effectivity of new healthimproving food products.
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Plasmid-mediated dissemination of antibiotic resistance among fecal Enterobacteriaceae in natural ecosystems may contribute to the persistence of antibiotic resistance genes in anthropogenically impacted environments. Plasmid transfer frequencies measured under laboratory conditions might lead to overestimation of plasmid transfer potential in natural ecosystems. This study assessed differences in the conjugative transfer of an IncP-1 (pKJK5) plasmid to three natural Escherichia coli strains carrying extended-spectrum beta-lactamases, by filter mating. Matings were performed under optimal laboratory conditions (rich LB medium and 37°C) and environmentally relevant temperatures (25, 15 and 9°C) or nutrient regimes mimicking environmental conditions and limitations (synthetic wastewater and soil extract). Under optimal nutrient conditions and temperature, two recipients yielded high transfer frequencies (5 × 10–1) while the conjugation frequency of the third strain was 1000-fold lower. Decreasing mating temperatures to psychrophilic ranges led to lower transfer frequencies, albeit all three strains conjugated under all the tested temperatures. Low nutritive media caused significant decreases in transconjugants (−3 logs for synthetic wastewater; −6 logs for soil extract), where only one of the strains was able to produce detectable transconjugants. Collectively, this study highlights that despite less-than-optimal conditions, fecal organisms may transfer plasmids in the environment, but the transfer of pKJK5 between microorganisms is limited mainly by low nutrient conditions.
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This booklet presents twenty-one impact stories of Ethiopian and Kenyan alumni of Dairy (Livestock) trainings in the Netherlands. The Dutch trainings consists of Master courses conducted by Van Hall Larenstein University of Applied Sciences (VHL) in Wageningen or Velp, short courses or diploma courses organised by Practical Trainings Centre (PTC+) in Oenkerk or Barneveld / Dairy Trainings Centre (DTC) in Oenkerk and short courses organised by Centre of Development Innovation – part of Wageningen University and Research Centre (WUR) in Wageningen. Most refresher course participants attended the Dutch trainings between 2010 and 2018. These alumni attended the Refresher Course entitled “Assessing the impact of Dutch knowledge institutions on performance of Netherlands alumni on dairy value chain governance in Kenya and Ethiopia” organised by Van Hall Larenstein University of Applied Sciences (VHL), Agrikom (alumni organisation) and Egerton University on 7-16 July 2019 in Nairobi and Nakuru, Kenya. Part of the refresher course was dedicated to a write shop about the impact of the Dutch training. It was written in collaboration with the professorship Climate Smart Dairy Value Chains.
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