Cities are confronted with more frequent heatwaves of increasing intensity discouraging people from using urban open spaces that are part of their daily lives. Climate proofing cities is an incremental process that should begin where it is needed using the most cost-efficient solutions to mitigate heat stress. However, for this to be achieved the factors that influence the thermal comfort of users, such as the layout of local spaces, their function and the way people use them needs to be identified first. There is currently little evidence available on the effectiveness of heat stress interventions in different types of urban space.The Cool Towns Heat Stress Measurement Protocol provides basic guidance to enable a full Thermal Comfort Assessment (TCA) to be conducted at street-level. Those involved in implementing climate adaptation strategies in urban areas, such as in redevelopments will find practical support to identify places where heat stress may be an issue and suggestions for effective mitigation measures. For others, such as project developers, and spatial designers such as landscape architects and urban planners it provides practical instructions on how to evaluate and provide evidence-based justification for the selection of different cooling interventions for example trees, water features, and shade sails, for climate proofing urban areas.
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Thermal comfort is determined by the combined effect of the six thermal comfort parameters: temperature, air moisture content, thermal radiation, air relative velocity, personal activity and clothing level as formulated by Fanger through his double heat balance equations. In conventional air conditioning systems, air temperature is the parameter that is normally controlled whilst others are assumed to have values within the specified ranges at the design stage. In Fanger’s double heat balance equation, thermal radiation factor appears as the mean radiant temperature (MRT), however, its impact on thermal comfort is often ignored. This paper discusses the impacts of the thermal radiation field which takes the forms of mean radiant temperature and radiation asymmetry on thermal comfort, building energy consumption and air-conditioning control. Several conditions and applications in which the effects of mean radiant temperature and radiation asymmetry cannot be ignored are discussed. Several misinterpretations that arise from the formula relating mean radiant temperature and the operative temperature are highlighted, coupled with a discussion on the lack of reliable and affordable devices that measure this parameter. The usefulness of the concept of the operative temperature as a measure of combined effect of mean radiant and air temperatures on occupant’s thermal comfort is critically questioned, especially in relation to the control strategy based on this derived parameter. Examples of systems which deliver comfort using thermal radiation are presented. Finally, the paper presents various options that need to be considered in the efforts to mitigate the impacts of the thermal radiant field on the occupants’ thermal comfort and building energy consumption.
Plasmid-mediated dissemination of antibiotic resistance among fecal Enterobacteriaceae in natural ecosystems may contribute to the persistence of antibiotic resistance genes in anthropogenically impacted environments. Plasmid transfer frequencies measured under laboratory conditions might lead to overestimation of plasmid transfer potential in natural ecosystems. This study assessed differences in the conjugative transfer of an IncP-1 (pKJK5) plasmid to three natural Escherichia coli strains carrying extended-spectrum beta-lactamases, by filter mating. Matings were performed under optimal laboratory conditions (rich LB medium and 37°C) and environmentally relevant temperatures (25, 15 and 9°C) or nutrient regimes mimicking environmental conditions and limitations (synthetic wastewater and soil extract). Under optimal nutrient conditions and temperature, two recipients yielded high transfer frequencies (5 × 10–1) while the conjugation frequency of the third strain was 1000-fold lower. Decreasing mating temperatures to psychrophilic ranges led to lower transfer frequencies, albeit all three strains conjugated under all the tested temperatures. Low nutritive media caused significant decreases in transconjugants (−3 logs for synthetic wastewater; −6 logs for soil extract), where only one of the strains was able to produce detectable transconjugants. Collectively, this study highlights that despite less-than-optimal conditions, fecal organisms may transfer plasmids in the environment, but the transfer of pKJK5 between microorganisms is limited mainly by low nutrient conditions.
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