Lecture in PhD Programme Life Science Education Research UMCU. Course Methods of Life Science Education Research. Utrecht, The Netherlands. abstract Audit trail procedures are applied as a way to check the validity of qualitative research designs, qualitative analyses, and the claims that are made. Audit trail procedures can be conducted based on the three criteria of visibility, comprehensibility, and acceptability (Akkerman et al., 2008). During an audit trail procedure, all documents and materials resulting from the data gathering and the data analysis are assessed by an auditor. In this presentation, we presented a summative audit trail procedure (Agricola, Prins, Van der Schaaf & Van Tartwijk, 2021), whereas in a second study we used a formative one (Agricola, Van der Schaaf, Prins & Van Tartwijk, 2022). For both studies, two different auditors were chosen. For the study presented in Agricola et al. (2021) the auditor was one of the PhD supervisors, while in that presented Agricola et al. (2022) was a junior researcher not involved in the project. The first auditor had a high level of expertise in the study’s topic and methodology. As a result, he was able to provide a professional and critical assessment report. Although the second auditor might be considered to be more objective than the first, as she was not involved in the project, more meetings were needed to explain the aim of the study and the aim of the audit trail procedure. There are many ideas about the criteria that qualitative studies should meet (De Kleijn en Van Leeuwen, 2018). I argue that procedures of checking for interrater agreement and understanding, the triangulation, and audit trail procedures can increase the internal validity of qualitative studies. Agricola, B. T., Prins, F. J., van der Schaaf, M. F., & van Tartwijk, J. (2021). Supervisor and Student Perspectives on Undergraduate Thesis Supervision in Higher Education. Scandinavian Journal of Educational Research, 65(5), 877-897. doi: https://doi.org/10.1080/00313831.2020.1775115 Agricola, B. T., van der Schaaf, M. F., Prins, F. J., & van Tartwijk, J. (2022). The development of research supervisors’ pedagogical content knowledge in a lesson study project. Educational Action Research. doi: https://doi.org/10.1080/09650792.2020.1832551 de Kleijn, R. A. M., & Van Leeuwen, A. (2018). Reflections and review on the audit procedure: Guidelines for more transparency. International Journal of Qualitative Methods, 17(1), 1-8. doi: https://doi.org/10.1177/1609406918763214 Akkerman, S., Admiraal, W., Brekelmans, M., & Oost, H. (2008). Auditing quality of research in social sciences. Quality & Quantity, 42(2), 257-274. doi: https://doi.org/10.1007/s11135-006-9044-4
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Formation of the pro-apoptotic death-inducing signaling complex (DISC) can be initiated in cancer cells via binding of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) to its two pro-apoptotic receptors, TRAIL receptor 1 (TRAIL-R1) and TRAIL-R2. Primary components of the DISC are trimerized TRAIL-R1/-R2, FADD, caspase 8 and caspase 10. The anti-apoptotic protein FLIP can also be recruited to the DISC to replace caspase 8 and form an inactive complex. Caspase 8/10 processing at the DISC triggers the caspase cascade, which eventually leads to apoptotic cell death. Besides TRAIL, TRAIL-R1- or TRAIL-R2-selective variants of TRAIL and agonistic antibodies have been designed. These ligands are of interest as anti-cancer agents since they selectively kill tumor cells. To increase tumor sensitivity to TRAIL death receptor-mediated apoptosis and to overcome drug resistance, TRAIL receptor ligands have already been combined with various therapies in preclinical models. In this review, we discuss factors influencing the initial steps of the TRAIL apoptosis signaling pathway, focusing on mechanisms modulating DISC assembly and caspase activation at the DISC. These insights will direct rational design of drug combinations with TRAIL receptor ligands to maximize DISC signaling. © 2009 Elsevier B.V. All rights reserved.
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Background: Drug resistance is a major problem in ovarian cancer. Triggering apoptosis using death ligands such as tumour necrosis factor-related apoptosis inducing ligand (TRAIL) might overcome chemoresistance. Methods: We investigated whether acquired cisplatin resistance affects sensitivity to recombinant human (rh) TRAIL alone or in combination with cisplatin in an ovarian cancer cell line model consisting of A2780 and its cisplatin-resistant subline CP70. Results: Combining cisplatin and rhTRAIL strongly enhanced apoptosis in both cell lines. CP70 expressed less caspase 8 protein, whereas mRNA levels were similar compared with A2780. Pre-exposure of particularly CP70 to cisplatin resulted in strongly elevated caspase 8 protein and mRNA levels. Caspase 8 mRNA turnover and protein stability in the presence or absence of cisplatin did not differ between both cell lines. Cisplatin-induced caspase 8 protein levels were essential for the rhTRAIL-sensitising effect as demonstrated using caspase 8 small-interfering RNA (siRNA) and caspase-8 overexpressing constructs. Cellular FLICE-inhibitory protein (c-FLIP) and p53 siRNA experiments showed that neither an altered caspase 8/c-FLIP ratio nor a p53-dependent increase in DR5 membrane expression following cisplatin were involved in rhTRAIL sensitisation. Conclusion: Cisplatin enhances rhTRAIL-induced apoptosis in cisplatin-resistant ovarian cancer cells, and induction of caspase 8 protein expression is the key factor of rhTRAIL sensitisation. © 2011 Cancer Research UK All rights reserved.
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Background and aims: Observational data indicate that diets rich in fruits and vegetables have a positive effect on inflammatory status, improve metabolic resilience and may protect against the development of non-communicable diseases. Nevertheless, experimental evidence demonstrating a causal relationship between nutrient intake (especially whole foods) and changes in metabolic health is scarce. This study investigated the pleiotropic effects of sulforaphane from broccoli sprouts, compared to pea sprouts, on biomarkers of endothelial function, inflammation and metabolic stress in healthy participants subjected to a standardized caloric challenge.Methods: In this double-blind, crossover, randomized, placebo-controlled trial 12 healthy participants were administered 16 g broccoli sprouts, or pea sprouts (placebo) followed by the standardized high-caloric drink PhenFlex given to disturb healthy homeostasis. Levels of inflammatory biomarkers and metabolic parameters were measured in plasma before and 2 h after the caloric overload.Results: Administration of broccoli sprouts promoted an increase in levels of CCL-2 induced by caloric load (p = 0.017). Other biomarkers (sICAM-1, sVCAM-1, hs-CRP, and IL-10) individually showed insignificant tendencies toward increase with administration of sulforaphane. Combining all studied biomarkers into the systemic low-grade inflammation score further confirmed upregulation of the inflammatory activity (p = 0.087) after sulforaphane. No significant effects on biomarkers of metabolic stress were detected.Conclusion: This study has demonstrated that sulforaphane facilitated development of a mild pro-inflammatory state during the caloric challenge, which could be suggestive of the onset of the hormetic response induced by this phytonutrient. The use of integrative outcomes measures such as the systemic low-grade inflammation score can be viewed as a more robust approach to study the subtle and pleiotropic effects of phytonutrients.Clinical trial registration:www.clinicaltrials.gov, identifier NCT05146804.Keywords: biomarkers; diet; glucoraphanin; hormesis; inflammation; nutrients; phenotypic flexibility; sulforaphane.
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This dissertation focuses on the question how money mules are recruited and which mechanisms play a role. Money mules are people who receive money from victims of online fraud. They are an indispensable link in the commission of financial-economic cybercrimes, such as phishing and bank helpdesk fraud, because they break the financial trail from victims to core members. The crucial role of money mules in the crime script and the possible consequences for young money mules themselves make them a valuable target group for scientific research. Almost no empirical research has been conducted into money mules and the involvement mechanisms of cybercrime. However, this knowledge is necessary for the development and application of prevention measures: interventions aimed at money mules disrupt the execution of various forms of online fraud, which can reduce victimization of cybercrime among citizens and businesses. In total, the dissertation consists of six empirical chapters, in which different research methods were used. This includes questionnaires and expert interviews, but also more unique and innovative methods such as online field experiments and analysis of police investigations into cybercriminal networks. The dissertation shows that money mules - still - form a crucial link in the world of financial-economic cybercrime. It is clear that this phenomenon manifests itself in different ways over time: online bank accounts, international bank accounts and crypto wallets are currently popular among cybercriminal networks because they offer even more anonymity than bank accounts at large traditional banks. This also means that money mules are also recruited for their identification cards instead of their bank card, which offenders use to open up accounts for themselves. It can be concluded that the social environment of money mules forms a criminal opportunity structure. Money mules are approached via-via and actively addressed; online on social media such as Instagram and Telegram but also offline on the street, at school or at the metro station. Social relationships therefore offer cybercriminal networks access to co-offenders, including money mules, and can explain why young people become involved in the world of cybercrime. Financial motivations play a role here, because money mules often look up to the luxurious lifestyle of criminals and give up bank account details in exchange for compensation. Risk perceptions regarding the likelihood of being caught and the consequences of money muling are low and money mules justify the criminal behavior. In addition to financial considerations, some recruiters also exert pressure or even threaten with violence. This reflects the heterogeneous nature of the target group and makes it clear that various involvement mechanisms play a role.
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This paper describes the work done to track the supply chains of sur-veillance technologies from the global North to African governments for illegal surveillance of their citizens. We conducted desk research to analyse the supply side of surveillance technology exported to African countries. Our preliminary findings show nine key exporting states-actors for surveillance technologies to Africa. These are China; European Union agencies, and member states France, Germany, Italy; Israel, United Kingdom, Russia, and the United States of Amer-ica. Regarding the specific surveillance technologies being transferred, each export-ing state tends to have a focus area, both in geographic area and within the five surveillance technology categories covered by this study. The paper identified six motives for state surveillance, including the suppliers' perspective. These are: 1) surveillance as legitimacy for state security, 2) surveillance for political gain, 3) surveillance as diplomacy, 4) surveillance as a tool for development, 5) Surveil-lance as neocolonialism, and 6) surveillance as business opportunity. Further re-search is needed to deepen the analysis of surveillance technologies exports to the African continent and the human rights violations.
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In deze rapportage worden de bevindingen gepresenteerd van een studie naar de huidige en toekomstige focus van de toeristisch-recreatieve beleidskaders en samenwerkingsmogelijkheden van Emsland-Drenthe.
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This pilot study explores the possibility of cognitive training software Neurotracker (NT), to have potential beneficial effects for Traumatic Brain Injury patients with Sensory Processing Disorder. Five subjects with TBI and SPD trained for 5 weeks/21 sessions with Neurotracker. Pre-post training cognitive tests (WAIS TMTA, TMTB, LNS) and surveys were conducted to measure possible cognitive differences with no statistical significant results. However, significant improvement in Neurotracker scores were found. =2.73, SD = 0.55) and positive changes associated with attention attention span, divided attention, (multiple) object tracking and motion sickness. LinkedIn: https://www.linkedin.com/in/bernard-de-roosz-28b96b125/
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