from the article: "Abstract: The oral mucosa is the first immune tissue that encounters allergens upon ingestion of food. We hypothesized that the bio-accessibility of allergens at this stage may be a key determinant for sensitization. Light roasted peanut flour was suspended at various pH in buffers mimicking saliva. Protein concentrations and allergens profiles were determined in the supernatants. Peanut protein solubility was poor in the pH range between 3 and 6, while at a low pH (1.5) and at moderately high pHs (>8), it increased. In the pH range of saliva, between 6.5 and 8.5, the allergens Ara h2 and Ara h6 were readily released, whereas Ara h1 and Ara h3 were poorly released. Increasing the pH from 6.5 to 8.5 slightly increased the release of Ara h1 and Ara h3, but the recovery remained low (approximately 20%) compared to that of Ara h2 and Ara h6 (approximately 100% and 65%, respectively). This remarkable difference in the extraction kinetics suggests that Ara h2 and Ara h6 are the first allergens an individual is exposed to upon ingestion of peanut-containing food. We conclude that the peanut allergens Ara h2 and Ara h6 are quickly bio-accessible in the mouth, potentially explaining their extraordinary allergenicity."
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Spectral imaging has many applications, from methane detection using satellites to disease detection on crops. However, spectral cameras remain a costly solution ranging from 10 thousand to 100 thousand euros for the hardware alone. Here, we present a low-cost multispectral camera (LC-MSC) with 64 LEDs in eight different colors and a monochrome camera with a hardware cost of 340 euros. Our prototype reproduces spectra accurately when compared to a reference spectrometer to within the spectral width of the LEDs used and the ±1σ variation over the surface of ceramic reference tiles. The mean absolute difference in reflectance is an overestimate of 0.03 for the LC-MSC as compared to a spectrometer, due to the spectral shape of the tiles. In environmental light levels of 0.5 W m−2 (bright artificial indoor lighting) our approach shows an increase in noise, but still faithfully reproduces discrete reflectance spectra over 400 nm–1000 nm. Our approach is limited in its application by LED bandwidth and availability of specific LED wavelengths. However, unlike with conventional spectral cameras, the pixel pitch of the camera itself is not limited, providing higher image resolution than typical high-end multi- and hyperspectral cameras. For sample conditions where LED illumination bands provide suitable spectral information, our LC-MSC is an interesting low-cost alternative approach to spectral imaging.
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Background: This study investigates patients’ use of eHealth services, their awareness of the availability of these services, and their intention to use them in primary care. It also examines patient characteristics and factors that influence the use of these services. Methods: A cross-sectional design using questionnaires was conducted. Based on the unified theory of acceptance and use of technology (UTAUT), the participants rated the two most common services. Descriptive analyses and linear correlation analyses were performed. A simple linear regression was conducted to identify factors influencing the participants’ intention to use eHealth services. Results: In total, 1203 participants with an average age of 43.7 years were surveyed. The participants’ usage rates varied, with the lowest at 2.4%, for measuring vital signs, and the highest at 47.4%, for booking appointments. The intentions to use the services ranged from 22.5%, for video consultations, to 46.6%, for prescription refill requests. Approximately 20% of the respondents were unaware of each service’s availability. Positive associations were found between all the constructs and the intention to use online services, with a younger age being the most significant factor. Conclusions: The use of and intention to use eHealth services varied greatly. The participants were often unaware of the availability of these services. Promoting the availability and benefits of eHealth services could enhance patient engagement in primary care settings.
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