The transition to a biobased economy necessitates utilizing renewable resources as a sustainable alternative to traditional fossil fuels. Bioconversion is a way to produce many green chemicals from renewables, e.g., biopolymers like PHAs. However, fermentation and bioconversion processes mostly rely on expensive, and highly refined pure substrates. The utilization of crude fractions from biorefineries, especially herbaceous lignocellulosic feedstocks, could significantly reduce costs. This presentation shows the microbial production of PHA from such a crude stream by a wild-type thermophilic bacterium Schlegelella thermodepolymerans [1]. Specifically, it uses crude xylose-rich fractions derived from a newly developed biorefinery process for grassy biomasses (the ALACEN process). This new stepwise mild flow-through biorefinery approach for grassy lignocellulosic biomass allows the production of various fractions: a fraction containing esterified aromatics, a monomeric xylose-rich stream, a glucose fraction, and a native-like lignin residue [2]. The crude xylose-rich fraction was free of fermentation-inhibiting compounds meaning that the bacterium S.thermodepolymerans could effectively use it for the production of one type of PHA, polyhydroxybutyrate. Almost 90% of the xylose in the refined wheat straw fraction was metabolized with simultaneous production of PHA, matching 90% of the PHA production per gram of sugars, comparable to PHA yields from commercially available xylose. In addition to xylose, S. thermodepolymerans converted oligosaccharides with a xylose backbone (xylans) into fermentable xylose, and subsequently utilized the xylose as a source for PHA production. Since the xylose-rich hydrolysates from the ALACEN process also contain some oligomeric xylose and minor hemicellulose-derived sugars, optimal valorization of the C5-fractions derived from the refinery process can be obtained using S. thermodepolymerans. This opens the way for further exploration of PHA production from C5-fractions out of a variety of herbaceous lignocellulosic biomasses using the ALACEN process combined with S. thermodepolymerans. Overall, the innovative utilization of renewable resources in fermentation technology, as shown herein, makes a solid contribution to the transition to a biobased economy.[1] W. Zhou, D.I. Colpa, H. Permentier, R.A. Offringa, L. Rohrbach, G.J.W. Euverink, J. Krooneman. Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans. Resources, Conservation and Recycling 194 (2023) 107006, ISSN 0921-3449, https://doi.org/10.1016/j.resconrec.2023.107006. [2] S. Bertran-Llorens, W.Zhou. M.A.Palazzo, D.I.Colpa, G.J.W.Euverink, J.Krooneman, P.J.Deuss. ALACEN: a holistic herbaceous biomass fractionation process attaining a xylose-rich stream for direct microbial conversion to bioplastics. Submitted 2023.
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In het dagelijks leven hebben we voortdurend met verschillende plastics te maken. Overal om ons heen komen we plastics tegen. Denk bijvoorbeeld aan verpakkingsmaterialen, flessen, flacons, kratten, tapijten en plastic draagtassen. Een leven zonder kunststoffen is in onze huidige maatschappij vrijwel ondenkbaar geworden. In 2014 werd er volgens Plastics Europe [1] wereldwijd maar liefst 311.000.000 ton aan kunststoffen geproduceerd, in 1950 was dit nog slechts 1.700.000 ton. Vanaf 1950 stijgt de wereldwijde productie van kunststoffen met gemiddeld 9% per jaar. Bij de huidige productiecapaciteit komt dit volgens Plastics Europe neer op gemiddeld 40 kg/jaar per hoofd van de wereldbevolking! Naar verwachting zal het gebruik van plastics verder toenemen naar gemiddeld 87 kg/jaar per hoofd van de wereldbevolking in het jaar 2050. In Nederland ligt het verbruik momenteel op gemiddeld 126 kg per inwoner. Maar volgens prognoses van VLEEM (Very Long Term Energy Environment Model) [2] zal dit groeien naar gemiddeld 220 kg per inwoner in 2050!! De toenemende vraag naar plastics wordt mede veroorzaakt omdat plastics op zich een gemakkelijk te verwerken materiaal is. Plastics zijn relatief goedkoop, hebben een lage specifieke dichtheid (t.o.v. bijvoorbeeld metalen), en zijn snel en gemakkelijk verwerkbaar.
Plastic is one of the biggest contributors to pollution of the planet. Due to the low recyclability of oil-based plastics, most plastic is being disposed into the environment. According to plastic oceans, 10 million tons of plastic are dumped into oceans annually. Currently, researchers are developing recycling methods for oil-based plastics and are looking for biobased alternatives. One of these alternatives are a class of polymers called polyhydroxyalkanoates (PHA’s). PHA’s differ from other biobased polymers, due to the process of fabrication. PHA’s are a natural polymer, acting as an energy and carbon storage for different strains of bacteria. Functioning as an energy storage, nature can break down PHA’s and PHA-based waste. (1) Different companies are working on PHA’s production, but a large deviations in physical properties were observed. This research aims to establish a relationship between the chemical and physical properties of the different PHA’s, using gel permeability chromatography (GPC), nuclear magnetic resonance (NMR) and gas chromatography-mass spectroscopy (GC-MS).
In het project wordt een nieuw door de HvA ontwikkelde methodiek (Open Collaborative Business Modelling methodiek, verder: ‘OCBM-methodiek’), toegepast om waardeproposities voor circulaire en biobased verpakkingen te ontwikkelen, samen met partijen uit de waardeketen. De inzet van biobased materialen is essentieel voor het terugdringen van het gebruik van fossiele plastics en – uiteindelijk – voor het bereiken van een volledig circulaire economie. De specifieke waardeketen waar het project zich op richt is die van verpakkingen op basis van Olifantsgras / Miscanthus. Projectpartner Vibers is een bedrijf dat dit gewas als grondstof gebruikt voor het produceren van o.a. verpakkingsmaterialen. Tijdens het project zal een viertal OCBM-sessies worden georganiseerd waarin Vibers in nauwe samenwerking met een wisselende groep ketenpartners en andere stakeholders een nieuwe waardepropositie formuleert. Projectpartner Kennisinstituut Duurzaam Verpakken (verder: KIDV) bewaakt in de OCBM-sessies de duurzaamheid van de ontwikkelde propositie en speelt een rol bij evaluatie van de OCBM-methodiek voor de verpakkingsindustrie. Het project levert daarmee twee belangrijke resultaten op: 1. Een met behulp van de OCBM-methodiek ontwikkelde waardepropositie voor een circulair business model waarin een biobased verpakking centraal staat; 2. Aanbevelingen voor het verfijnen van de OCBM-methodiek: specifieke aandachtspunten voor het ontwikkelen van innovatieve, circulaire business modellen met behulp van deze methodiek.
Currently, many novel innovative materials and manufacturing methods are developed in order to help businesses for improving their performance, developing new products, and also implement more sustainability into their current processes. For this purpose, additive manufacturing (AM) technology has been very successful in the fabrication of complex shape products, that cannot be manufactured by conventional approaches, and also using novel high-performance materials with more sustainable aspects. The application of bioplastics and biopolymers is growing fast in the 3D printing industry. Since they are good alternatives to petrochemical products that have negative impacts on environments, therefore, many research studies have been exploring and developing new biopolymers and 3D printing techniques for the fabrication of fully biobased products. In particular, 3D printing of smart biopolymers has attracted much attention due to the specific functionalities of the fabricated products. They have a unique ability to recover their original shape from a significant plastic deformation when a particular stimulus, like temperature, is applied. Therefore, the application of smart biopolymers in the 3D printing process gives an additional dimension (time) to this technology, called four-dimensional (4D) printing, and it highlights the promise for further development of 4D printing in the design and fabrication of smart structures and products. This performance in combination with specific complex designs, such as sandwich structures, allows the production of for example impact-resistant, stress-absorber panels, lightweight products for sporting goods, automotive, or many other applications. In this study, an experimental approach will be applied to fabricate a suitable biopolymer with a shape memory behavior and also investigate the impact of design and operational parameters on the functionality of 4D printed sandwich structures, especially, stress absorption rate and shape recovery behavior.
Plastic products are currently been critically reviewed due to the growing awareness on the related problems, such as the “plastic soup”. EU has introduced a ban for a number of single-use consumer products and fossil-based polymers coming in force in 2021. The list of banned products are expected to be extended, for example for single-use, non-compostable plastics in horticulture and agriculture. Therefore, it is crucial to develop sustainable, biodegradable alternatives. A significant amount of research has been performed on biobased polymers. However, plastics are made from a polymer mixed with other materials, additives, which are essential for the plastics production and performance. Development of biodegradable solutions for these additives is lacking, but is urgently needed. Biocarbon (Biochar), is a high-carbon, fine-grained residue that is produced through pyrolysis processes. This natural product is currently used to produce energy, but the recent research indicate that it has a great potential in enhancing biopolymer properties. The biocarbon-biopolymer composite could provide a much needed fully biodegradable solution. This would be especially interesting in agricultural and horticultural applications, since biocarbon has been found to be effective at retaining water and water-soluble nutrients and to increase micro-organism activity in soil. Biocarbon-biocomposite may also be used for other markets, where biodegradability is essential, including packaging and disposable consumer articles. The BioADD consortium consists of 9 industrial partners, a branch organization and 3 research partners. The partner companies form a complementary team, including biomass providers, pyrolysis technology manufacturers and companies producing products to the relevant markets of horticulture, agriculture and packaging. For each of the companies the successful result from the project will lead to concrete business opportunities. The support of Avans, University of Groningen and Eindhoven University of Technology is essential in developing the know-how and the first product development making the innovation possible.
