A bioaugmentation approach was used to enhance the performance of anaerobic digestion (AD) using cow manure (CM) as the substrate in a continuous system. To obtain the desirable microbial culture for bioaugmentation, a biochemical methane potential test (BMP) was used to evaluate three commonly used inocula namely (1) municipal solid waste (MSW), (2) wastewater treatment plant (WWTP), and (3) cow manure digester (CMMD) for their hydrolytic capacity. The highest lignocellulose removal (56% for cellulose and 50% for hemicellulose) and the most profusion of cellulolytic bacteria were obtained when CM was inoculated with CMMD. CMMD was thus used as the seed inoculum in a continuously operated reactor (Ra) with the fiber fraction of CM as the substrate to further enrich cellulolytic microbes. After 100 days (HRT: 30 days), the Bacteria fraction mainly contained Ruminofilibacter, norank_o_SBR1031, Treponema, Acetivibrio. Surprisingly, the Archaea fraction contained 97% ‘cellulolytic archaea’ norank_c_Bathyarchaeia (Phylum Bathyarchaeota). This enriched consortium was used in the bioaugmentation experiment. A positive effect of bioaugmentation was verified, with a substantial daily methane yield (DMY) enhancement (24.3%) obtained in the bioaugmented reactor (Rb) (179 mL CH4/gVS/d) than that of the control reactor (Rc) (144 mL CH4/gVS/d) (P < 0.05). Meanwhile, the effluent of Rb enjoyed an improved cellulose reduction (14.7%) than that of Rc, whereas the amount of hemicellulose remained similar in both reactors' effluent. When bioaugmentation stopped, its influence on the hydrolysis and methanogenesis sustained, reflected by an improved DMY (160 mL CH4/gVS/d) and lower cellulose content (53 mg/g TS) in Rb than those in Rc (DMY 144 mL/CH4/gVS/d and cellulose content 63 mg/g TS, respectively). The increased DMY of the continuous reactor seeded with a specifically enriched consortium able to degrade the fiber fraction in CM shows the feasibility of applying bioaugmentation in AD of CM.
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The Bio-P2G-program (Bio-Power to Gas) at the Hanze University of AppliedSciences evaluates the technologic feasibility of the biological reduction of carbondioxide with hydrogen to methane (biomethanation: 1 CO2 + 4 H2 -> CH4 + 2 H2O)Chemically, this process is known as the Sabatier reaction, but within anaerobicdigestion the biological methanation is catalyzed by a specific group ofmicroorganisms: the hydrogenotrophic methanogens.
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Wind and solar power generation will continue to grow in the energy supply of the future, but its inherent variability (intermittency) requires appropriate energy systems for storing and using power. Storage of possibly temporary excess of power as methane from hydrogen gas and carbon dioxide is a promising option. With electrolysis hydrogen gas can be generated from (renewable) power. The combination of such hydrogen with carbon dioxide results in the energy carrier methane that can be handled well and may may serve as carbon feedstock of the future. Biogas from biomass delivers both methane and carbon dioxide. Anaerobic microorganisms can make additional methane from hydrogen and carbon dioxide in a biomethanation process that compares favourably with its chemical counterpart. Biomethanation for renewable power storage and use makes appropriate use of the existing infrastructure and knowledge base for natural gas. Addition of hydrogen to a dedicated biogas reactor after fermentation optimizes the biomethanation conditions and gives maximum flexibility. The low water solubility of hydrogen gas limits the methane production rate. The use of hollow fibers, nano-bubbles or better-tailored methane-forming microorganisms may overcome this bottleneck. Analyses of patent applications on biomethanation suggest a lot of freedom to operate. Assessment of biomethanation for economic feasibility and environmental value is extremely challenging and will require future data and experiences. Currently biomethanation is not yet economically feasible, but this may be different in the energy systems of the near future.
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Excess of renewable electricity from wind turbines or solar panels is used for electrolysis of water. To store this renewable energy as methane, the hydrogen is fed to an anaerobic digester to stimulate biological methanation by hydrogenotrophic methanogens. This workpackage focusses on the best ways for hydrogen delivery and the community changes in a biomethanation reactor as a result of hydrogen supply.
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Excess of renewable electricity from wind turbines or solar panels is used for electrolysis of water. To store this renewable energy as methane, the hydrogen is fed to an anaerobic digester to stimulate biological methanation by hydrogenotrophic methanogens. These work packages focus on the best ways for hydrogen delivery and the community changes in a biomethanation reactor as a result of hydrogen supply.
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For centuries, natural gas has been one of humanity’s main energy sources. The gas sector is still heavily reliant on natural gas production; however, as natural gas fields contain only a finite quantity of gas, its continued extraction is leading to the resource’s depletion. Furthermore, natural gas production has become a subject of debate, with many considering continued utilisation incompatible with the achievement of international and European climate goals. The need for alternative gases that are less damaging to the environment is becoming increasingly evident. Biomethane has shown itself to be a reliable alternative to natural gas, and if sourced and manufactured responsibly results in no new CO2 emissions. Another alternative, hydrogen, can, through the process of methanisation, be converted into synthetic natural gas (SNG). This chapter discusses the legal aspects of the production and use of biomethane, hydrogen and SNG.
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Source Segregation (SS) is a novel strategy in dairy housing that can reduce emissions and separate organic matter and nutrients more efficiently than traditional slurry solid-liquid separation. The anaerobic digestion (AD) methane yield of the SS fractions, however, is unknown. We aimed at unveiling the biomethane yield of these fractions by conducting AD experiments under different configurations: batch, continuous feeding, and fed-batch. In the batch test, the solid (SF) and liquid fraction (LF) from the SS system, a slurry collected from the pit (CS), and a self-made slurry (MF) were used as substrates. The results showed that the specific CH4 yields of the SF and MF were in same range and both higher than the CS. We concluded that SS can increase the CH4 yield of dairy excreta mainly by reducing losses in the animal house. The SF and MF were then compared in a continuously-fed thermophilic test, where SF had a higher specific (174 compared to 105 NL kg-1 VS) and volumetric (12.2 compared to 9.9 NL CH4 kg-1 excreta) yields. We concluded that the SF can effectively substitute slurry in AD without compromising the yield, possibly increasing economic viability by reducing transport costs and reactor size. Further, SF produced 356 NL CH4 kg-1 VS and a digestate with 1.8% lower dry matter in the fed-batch as compared to continuous feeding. Continuously stirred fed-batch can thus increase the CH4 yield of the SF and reduce the DM of its digestate potentially contributing to lower emissions in storage and field application.
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The project BioP2M came to a close in June 2019 after a consortium of stakeholders in the field of energy transition worked together to research the diverse role of Methane. In this report the results are presented and future plans are discussed.
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Locally produced methane, - either as biomethane or power-to-gas product, has to be stored to provide a reliable gas source for the fluctuating demand of any local gas distribution network. Additionally, methane is a prominent transportation fuel but its suitability for vehicular application depends on the ability to store an adequate amount in the onboard fuel tank. Adsorption in porous materials could enable a simple, safe and cost-effective method for storing methane at ambient temperature and at reasonably low pressure. In this project we study and test the main thermodynamic and kinetic characteristics of methane adsorption and desorption on activated carbon. Both calculations and measurements are performed to enhance our knowledge about the general performance and the cyclic behavior of the adsorption and desorption processes.
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In het hoofdstuk wordt ingegaan op de innovaties in de Europese gassector, met een speciale focus op de invoeding van groen gas (ook wel biomethaan) in het aardgassysteem. Er wordt een algemeen juridisch kader geschetst en er vindt een rechtsvergelijking plaats van de nationale rechtsordes aangaande Duitsland, Denemarken en Nederland.
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