A bioaugmentation approach was used to enhance the performance of anaerobic digestion (AD) using cow manure (CM) as the substrate in a continuous system. To obtain the desirable microbial culture for bioaugmentation, a biochemical methane potential test (BMP) was used to evaluate three commonly used inocula namely (1) municipal solid waste (MSW), (2) wastewater treatment plant (WWTP), and (3) cow manure digester (CMMD) for their hydrolytic capacity. The highest lignocellulose removal (56% for cellulose and 50% for hemicellulose) and the most profusion of cellulolytic bacteria were obtained when CM was inoculated with CMMD. CMMD was thus used as the seed inoculum in a continuously operated reactor (Ra) with the fiber fraction of CM as the substrate to further enrich cellulolytic microbes. After 100 days (HRT: 30 days), the Bacteria fraction mainly contained Ruminofilibacter, norank_o_SBR1031, Treponema, Acetivibrio. Surprisingly, the Archaea fraction contained 97% ‘cellulolytic archaea’ norank_c_Bathyarchaeia (Phylum Bathyarchaeota). This enriched consortium was used in the bioaugmentation experiment. A positive effect of bioaugmentation was verified, with a substantial daily methane yield (DMY) enhancement (24.3%) obtained in the bioaugmented reactor (Rb) (179 mL CH4/gVS/d) than that of the control reactor (Rc) (144 mL CH4/gVS/d) (P < 0.05). Meanwhile, the effluent of Rb enjoyed an improved cellulose reduction (14.7%) than that of Rc, whereas the amount of hemicellulose remained similar in both reactors' effluent. When bioaugmentation stopped, its influence on the hydrolysis and methanogenesis sustained, reflected by an improved DMY (160 mL CH4/gVS/d) and lower cellulose content (53 mg/g TS) in Rb than those in Rc (DMY 144 mL/CH4/gVS/d and cellulose content 63 mg/g TS, respectively). The increased DMY of the continuous reactor seeded with a specifically enriched consortium able to degrade the fiber fraction in CM shows the feasibility of applying bioaugmentation in AD of CM.
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This conference paper deals with various organizations and pilot initiatives regarding the theme of sustainability.
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Innovations are required in urban infrastructures due to the pressing needs for mitigating climate change and prevent resource depletion. In order to address the slow pace of innovation in urban systems, this paper analyses factors involved in attempts to introduce novel sanitary systems. Today new requirements are important: sanitary systems should have an optimal energy/climate performance, with recovery of resources, and with fewer emissions. Anaerobic digestion has been suggested as an alternative to current aerobic waste water treatment processes. This paper presents an overview of attempts to introduce novel anaerobic sanitation systems for domestic sanitation. The paper identifies main factors that contributed to a premature termination of such attempts. Especially smaller scale anaerobic sanitation systems will probably not be able to compete economically with traditional sewage treatment. However, anaerobic treatment has various advantages for mitigating climate change, removing persistent chemicals, and for the transition to a circular economy. The paper concludes that loss avoidance, both in the sewage system and in the waste water treatment plants, should play a key role in determining experiments that could lead to a transition in sanitation. http://dx.doi.org/10.13044/j.sdewes.d6.0214 LinkedIn: https://www.linkedin.com/in/karel-mulder-163aa96/
MULTIFILE
Phosphorus is an essential element for life, whether in the agricultural sector or in the chemical industry to make products such as flame retardants and batteries. Almost all the phosphorus we use are mined from phosphate rocks. Since Europe scarcely has any mine, we therefore depend on imported phosphate, which poses a risk of supply. To that effect, Europe has listed phosphate as one of its main critical raw materials. This creates a need for the search for alternative sources of phosphate such as wastewater, since most of the phosphate we use end up in our wastewater. Additionally, the direct discharge of wastewater with high concentration of phosphorus (typically > 50 ppb phosphorus) creates a range of environmental problems such as eutrophication . In this context, the Dutch start-up company, SusPhos, created a process to produce biobased flame retardants using phosphorus recovered from municipal wastewater. Flame retardants are often used in textiles, furniture, electronics, construction materials, to mention a few. They are important for safety reasons since they can help prevent or spread fires. Currently, almost all the phosphate flame retardants in the market are obtained from phosphate rocks, but SusPhos is changing this paradigm by being the first company to produce phosphate flame retardants from waste. The process developed by SusPhos to upcycle phosphate-rich streams to high-quality flame retardant can be considered to be in the TRL 5. The company seeks to move further to a TRL 7 via building and operating a demo-scale plant in 2021/2022. BioFlame proposes a collaboration between a SME (SusPhos), a ZZP (Willem Schipper Consultancy) and HBO institute group (Water Technology, NHL Stenden) to expand the available expertise and generate the necessary infrastructure to tackle this transition challenge.
Cell-based production processes in bioreactors and fermenters need to be carefully monitored due to the complexity of the biological systems and the growth processes of the cells. Critical parameters are identified and monitored over time to guarantee product quality and consistency and to minimize over-processing and batch rejections. Sensors are already available for monitoring parameters such as temperature, glucose, pH, and CO2, but not yet for low-concentration substances like proteins and nucleic acids (DNA). An interesting critical parameter to monitor is host cell DNA (HCD), as it is considered an impurity in the final product (downstream process) and its concentration indicates the cell status (upstream process). The Molecular Biosensing group at the Eindhoven University of Technology and Helia Biomonitoring are developing a sensor for continuous biomarker monitoring, based on Biosensing by Particle Motion. With this consortium, we want to explore whether the sensor is suitable for the continuous measurement of HCD. Therefore, we need to set-up a joint laboratory infrastructure to develop HCD assays. Knowledge of how cells respond to environmental changes and how this is reflected in the DNA concentration profile in the cell medium needs to be explored. This KIEM study will enable us to set the first steps towards continuous HCD sensing from cell culture conditions controlling cell production processes. It eventually generates input for machine learning to be able to automate processes in bioreactors and fermenters e.g. for the production of biopharmaceuticals. The project entails collaboration with new partners and will set a strong basis for subsequent research projects leading to scientific and economic growth, and will also contribute to the human capital agenda.
Aiming for a more sustainable future, biobased materials with improved performance are required. For biobased vinyl polymers, enhancing performance can be achieved by nanostructuring the material, i.e. through the use of well-defined (multi-)block, gradient, graft, comb, etc., copolymer made by controlled radical polymerization (CRP). Dispoltec has developed a new generation of alkoxyamines, which suppress termination and display enhanced end group stability compared to state-of-art CRP. Hence, these alkoxyamines are particularly suited to provide access to such biobased nanostructured materials. In order to produce alkoxyamines in a more environmentally benign and efficient manner, a photo-chemical step is beneficial for the final stage in their synthesis. Photo-flow chemistry as a process intensification technology is proposed, as flow chemistry inherently leads to more efficient reactions. In particular, photo-flow offers the benefit of significantly enhancing reactant concentrations and reducing batch times due to highly improved illumination. The aim of this project is to demonstrate at lab scale the feasibility of producing the new generation of alkoxy-amines via a photo-flow process under industrially relevant conditions regarding concentration, duration and efficiency. To this end, Zuyd University of Applied Sciences (Zuyd), CHemelot Innovation and Learning Labs (CHILL) and Dispoltec BV want to enter into a collaboration by combining the expertise of Dispoltec on alkoxyamines for CRP with those of Zuyd and CHILL on microreactor technology and flow chemistry. Improved access to these alkoxyamines is industrially relevant for initiator manufacturers, as well as producers of biobased vinyl polymers and end-users aiming to enhance performance through nanostructuring biobased materials. In addition, access in this manner is a clear demonstration for the high industrial potential of photo-flow chemistry as sustainable manufacturing tool. Further to that, students and professionals working together at CHILL will be trained in this emerging, industrially relevant and sustainable processing tool.
