This research report contains the findings of an international study consisting of three online ‘living’ surveys. The surveys focused on how the COVID-19 pandemic has impacted sign language interpreters’ working practices, how this was experienced by them, and how digital disruption caused by the pandemic is impacting and innovating the sign language interpreting profession. The study was carried out between April 2020 and July 2020; the largest contingent of respondents over all three surveys were from the U.S., followed by the UK, the Netherlands, Germany, Finland and Belgium. Respondents commented that the crisis will probably accelerate the need for remote interpreting training in interpreter training programs. Another resurfacing issue was the perceived need for sign language interpreting students to have face-to-face practice and live mentoring. Respondents commented on what benefits they thought remote interpreting might bring to the table, both for themselves and for deaf people. In general, the most significant benefits that were mentioned were flexibility and the possibility to improve efficiency and availability of sign language interpreting services. Notwithstanding these benefits, a significant number of respondents claimed that remote interpreting is more stressful than face-to-face interpreting and requires a heavier cognitive load.
To accelerate differentiation between Staphylococcus aureus and Coagulase Negative Staphylococci (CNS), this study aimed to compare six different DNA extraction methods from 2 commonly used blood culture materials, i.e. BACTEC and Bact/ALERT. Furthermore, we analyzed the effect of reduced blood culture times for detection of Staphylococci directly from blood culture material. A real-time PCR duplex assay was used to compare 6 different DNA isolation protocols on two different blood culture systems. Negative blood culture material was spiked with MRSA. Bacterial DNA was isolated with: automated extractor EasyMAG (3 protocols), automated extractor MagNA Pure LC (LC Microbiology Kit MGrade), a manual kit MolYsis Plus, and a combination between MolYsis Plus and the EasyMAG. The most optimal isolation method was used to evaluate reduced bacterial culture times. Bacterial DNA isolation with the MolYsis Plus kit in combination with the specific B protocol on the EasyMAG resulted in the most sensitive detection of S.aureus, with a detection limit of 10 CFU/ml, in Bact/ALERT material, whereas using BACTEC resulted in a detection limit of 100 CFU/ml. An initial S.aureus load of 1 CFU/ml blood can be detected after 5 hours of culture in Bact/ALERT3D by combining the sensitive isolation method and the tuf LightCycler assay.
According to the essayist and memoirist Rebecca Solnit, to be lost is 'to be fully present, and to be fully present is to be capable of being in uncertainty and mystery.' Solnit considers being lost 'a psychic state achievable through geography'. The flip-side is that geography can also prevent one from getting lost. It is easier to find one's way in the grid-like cities of the new world than in the swerving cobblestone streets of the old. A similar evolution of efficiency is happening on the web. The new world, in this analogy, is Web 2.0, and its shape is neither grid- nor weblike. Increasingly, it's a point of convergence.
MULTIFILE
Residential electricity distribution grid capacityis based on the typical peak load of a house and the loadsimultaneity factor. Historically, these values have remainedpredictable, but this is expected to change due to increasingelectric heating using heat pumps and rooftop solar panelelectricity generation. It is currently unclear how this increasein electrification will impact household peak load and loadsimultaneity, and hence the required grid capacity of residentialelectricity distribution grids. To gain better insight, transformerand household load measurements were taken in an all-electricneighborhood over a period of three years. These measurementswere analyzed to determine how heat pumps and solar panelswill alter peak load and load simultaneity and hence gridcapacity design parameters. Moreover, the potential for smartgrids to reduce peak loads and load simultaneity, and hencereduce required grid capacities, was examined.
