In sexual assault cases, the retrieved DNA quantity and sampling location from the victim’s underwear may provide valuable information for activity level evaluative reporting. DNA can transfer from site to site on an exhibit, or be lost within packaging, complicating interpretation. Experiments are needed to investigate these factors. This preliminary study compared two cleaning methods to prepare undergarments for such experimentation: hand-washing with warm water and washing with bleach before rinsing. Results show a significantly lower quantity of DNA on washed underwear using both methods. Warm-water hand-washing, the more straightforward method, was selected for further experimentation.
This paper reveals how the automatising of protocols ignited a public conflict between Dutch banks and their Small and Medium-sized Enterprise (SME) clients in the years after the Global Financial Crisis. The bank’s “infirmary departments” for Financial Restructuring and Recovery (FR&R) were accused of (mal)treating SMEs. The conflict resulted in no formal regulatory or legal change despite public support. Instead, the banks created self-regulation to improve communication with SMEs, leading to shifts in governing FR&R for SMEs. This way, the banks mitigated significant negative symptoms of automation and solved the conflict with the SMEs while keeping FR&R and ongoing automation intact. The research uses an interdisciplinary analytical framework to understand national financial conflicts in a digitalised (business) world. It contributes to the theory of institutionalising values in discursive contests between action fields. The paper highlights the material and causes of normative conflicts of interest among critical actors in established public-private networks through discourse analysis and process tracing.
OBJECTIVES: To compare low contrast detail (LCD) detectability and radiation dose for routine paediatric chest X-ray (CXR) imaging protocols among various hospitals.METHODS: CDRAD 2.0 phantom and medical grade polymethyl methacrylate (PMMA) slabs were used to simulate the chest region of four different paediatric age groups. Radiographic acquisitions were undertaken on 17 X-ray machines located in eight hospitals using their existing CXR protocols. LCD detectability represented by image quality figure inverse (IQF inv) was measured physically using the CDRAD analyser software. Incident air kerma (IAK) measurements were obtained using a solid-state dosimeter. RESULTS: The range of IQF inv, between and within the hospitals, was 1.40-4.44 and 1.52-2.18, respectively for neonates; 0.96-4.73 and 2.33-4.73 for a 1-year old; 0.87-1.81 and 0.98-1.46 for a 5-year old and 0.90-2.39 and 1.27-2.39 for a 10-year old. The range of IAK, between and within the hospitals, was 8.56-52.62 μGy and 21.79-52.62 μGy, respectively for neonates; 5.44-82.82 μGy and 36.78-82.82 μGy for a 1-year old; 10.97-59.22 μGy and 11.75-52.94 μGy for a 5-year old and 13.97-100.77 μGy and 35.72-100.77 μGy for a 10-year old. CONCLUSIONS: Results show considerable variation, between and within hospitals, in the LCD detectability and IAK. Further radiation dose optimisation for the four paediatric age groups, especially in hospitals /X-ray rooms with low LCD detectability and high IAK, are required.
An important line of research within the Center of Expertise HAN BioCentre is the development of the nematode Caenorhabditis elegans as an animal testing replacement organism. In the context of this, us and our partners in the research line Elegant! (project number. 2014-01-07PRO) developed reliable test protocols, data analysis strategies and new technology, to determine the expected effects of exposure to specific substances using C. elegans. Two types of effects to be investigated were envisaged, namely: i) testing of possible toxicity of substances to humans; and ii) testing for potential health promotion of substances for humans. An important deliverable was to show that the observed effects in the nematode can indeed be translated into effects in humans. With regard to this aspect, partner Preventimed has conducted research in obesity patients during the past year into the effect of a specific cherry extract that was selected as promising on the basis of the study with C. elegans. This research is currently being completed and a scientific publication will have to be written. The Top Up grant is intended to support the publication of the findings from Elegant! and also to help design experimental protocols that enable students to become acquainted with alternative medical testing systems to reduce the use of laboratory animals during laboratory training.
Organ-on-a-chip technology holds great promise to revolutionize pharmaceutical drug discovery and development which nowadays is a tremendously expensive and inefficient process. It will enable faster, cheaper, physiologically relevant, and more reliable (standardized) assays for biomedical science and drug testing. In particular, it is anticipated that organ-on-a-chip technology can substantially replace animal drug testing with using the by far better models of true human cells. Despite this great potential and progress in the field, the technology still lacks standardized protocols and robust chip devices, which are absolutely needed for this technology to bring the abovementioned potential to fruition. Of particular interest is heart-on-a-chip for drug and cardiotoxicity screening. There is presently no preclinical test system predicting the most important features of cardiac safety accurately and cost-effectively. The main goal of this project is to fabricate standardized, robust generic heart-on-a-chip demonstrator devices that will be validated and further optimized to generate new physiologically relevant models to study cardiotoxicity in vitro. To achieve this goal various aspects will be considered, including (i) the search for alternative chip materials to replace PDMS, (ii) inner chip surface modification and treatment (chemistry and topology), (iii) achieving 2D/3D cardiomyocyte (long term) cell culture and cellular alignment within the chip device, (iv) the possibility of integrating in-line sensors in the devices and, finally, (v) the overall chip design. The achieved standardized heart-on-a-chip technology will be adopted by pharmaceutical industry. This proposed project offers a unique opportunity for the Netherlands, and Twente in particular, which has relevant expertise, potential, and future perspective in this field as it hosts world-leading companies pioneering various core aspects of the technology that are relevant for organs-on-chips, combined with two world-leading research institutes within the University of Twente.
The SPRONG group, originating from the CoE KennisDC Logistiek, focuses on 'Low Impact in Lastmile Logistics' (LILS). The LILS group conducts practical research with local living labs and learning communities. There is potential for more collaboration and synergy for nationwide scaling of innovations, which is currently underutilized. LILS aims to make urban logistics more sustainable and facilitate necessary societal transitions. This involves expanding the monodisciplinary and regional scope of CoE KennisDC Logistiek to a multidisciplinary and supra-regional approach, incorporating expertise in spatial planning, mobility, data, circularity, AI, behavior, and energy. The research themes are:- Solutions in scarce space aiming for zero impact;- Influencing behavior of purchasers, recipients, and consumers;- Opportunities through digitalization.LILS seeks to increase its impact through research and education beyond its regions. Collaboration between BUas, HAN, HR, and HvA creates more critical mass. LILS activities are structured around four pillars:- Developing a joint research and innovation program in a roadmap;- Further integrating various knowledge domains on the research themes;- Deepening methodological approaches, enhancing collaboration between universities and partners in projects, and innovating education (LILS knowledge hub);- Establishing an organizational excellence program to improve research professionalism and quality.These pillars form the basis for initiating and executing challenging, externally funded multidisciplinary research projects. LILS is well-positioned in regions where innovations are implemented and has a strong national and international network and proven research experience.Societal issue:Last-mile logistics is crucial due to its visibility, small deliveries, high costs, and significant impact on emissions, traffic safety, and labor hours. Lastmile activities are predicted to grow a 20% growth in the next decade. Key drivers for change include climate agreements and energy transitions, urban planning focusing on livability, and evolving retail landscapes and consumer behavior. Solutions involve integrating logistics with spatial planning, influencing purchasing behavior, and leveraging digitalization for better data integration and communication. Digital twins and the Physical Internet concept can enhance efficiency through open systems, data sharing, asset sharing, standardization, collaboration protocols, and modular load units.Key partners: Buas, HR, HAN, HvAPartners: TNO, TU Delft, Gemeente Rotterdam, Hoger Onderwijs Drechtsteden, Significance, Metropolitan Hub System, evofenedex, Provincie Gelderland, Duurzaam Bereikbaar Heijendaal, Gemeente Alphen aan den Rijn, Radboud Universiteit, I&W - DMI, DHL, TLN, Noorderpoort, Fabrications, VUB, Smartwayz, RUG, Groene Metropoolregio.
