Zoekresultaten

Short communications: Exploring temporal fluorescent changes in the composition of human semen stains

Semen traces are considered important pieces of evidence in forensic investigations, especially those involving sexsual offenses. Recently, our research group developed a fluorescence-based technique to accurately determine the age of semen traces. However, the specific compounds resonsible for the fluoresescent behaviour of ageing semens remain unknown. As such, in this exploratory study, the aim is to identify the components associated with the fluorescent behavior of ageing semen traces. In this investigation semen stains and various biofluorophores commonly found in body fluids were left to aged for 0, 2, 4, 7, 14 and 21 days. Subsequently, thin-layer chromatography (TLC) and ultra-performance liquid chromatography (UPLC) mass spectrometry were performed to identify the biofluorophores present in semen. Several contributors to the autofluorescence could be identified in semen stain, these include tryptophan, kynurenine, kynurenic acid, and norharman. The study sheds light on the.

Identifying 3D Objects.

Comparing mixed-media and conventional slow-sand filters for arsenic removal from groundwater

Arsenic contamination of groundwater is a major public health concern worldwide. The problem has been reported mainly in southern Asia and, especially, in Bangladesh. Slow-sand filters (SSF) augmented with iron were proven to be a simple, low-cost and decentralized technique for the treatment of arsenic-contaminated sources. In this research, three pilot-scale SSF (flowrate 6 L·h−1) were tested regarding their capability of removing arsenic from groundwater in conditions similar to those found in countries like Bangladesh (70 µg As(III) L−1, 26 °C). From the three, two filters were prepared with mixed media, i.e., sand mixed with corrosive iron matter (CIM filter) and iron-coated sand (ICS filter), and a third conventional SSF was used as a reference. The results obtained showed that the CIM filter could remove arsenic below the World Health Organization (WHO) guideline concentration of 10 µg·L−1, even for inlet concentrations above 150 µg·L−1. After 230 days of continuous operation the arsenic concentration in the effluent started increasing, indicating depletion or saturation of the CIM layer. The effluent arsenic concentration, however, never exceeded the Bangladeshi standard of 50 µg·L−1 throughout the whole duration of the experiments.

Analysis of the fluorescent properties of vaginal fluid upon ageing

Detection and identification of body fluids are crucial aspects of forensic investigations, aiding in crime scene reconstructions and providing important leads. Although many methods have been developed for these purposes, no method is currently in use in the forensic field that allows rapid, non-contact detection and identification of vaginal fluids directly at the crime scene. The development of such technique is mainly challenged by the complex chemistry of the constituents, which can differ between donors and exhibits changes based on woman’s menstrual cycle. The use of fluorescence spectroscopy has shown promise in this area for other biological fluids. Therefore, the aim of this study was to identify specific fluorescent signatures of vaginal fluid with fluorescence spectroscopy to allow on-site identification. Additionally, the fluorescent properties were monitored over time to gain insight in the temporal changes of the fluorescent spectra of vaginal fluid. The samples were excited at wavelengths ranging from 200 to 600 nm and the induced fluorescence emission was measured from 220 to 700 nm. Excitation and emission maps (EEMs) were constructed for eight donors at seven time points after donation. Four distinctive fluorescence peaks could be identified in the EEMs, indicating the presence of proteins, fluorescent oxidation products (FOX), and an unidentified component as the dominant contributors to the fluorescence. To further asses the fluorescence characteristics of vaginal fluid, the fluorescent signatures of protein and FOX were used to monitor protein and lipid oxidation reactions over time. The results of this study provide insights into the intrinsic fluorescent properties of vaginal fluid over time which could be used for the development of a detection and identification method for vaginal fluids. Furthermore, the observed changes in fluorescence signatures over time could be utilized to establish an accurate ageing model.

Specific fluorescent signatures for body fluid identification using fluorescence spectroscopy

Non-invasive, rapid, on-site detection and identification of body fluids is highly desired in forensic investigations. The use of fluorescence-based methods for body fluid identification, have so far remain relatively unexplored. As such, the fluorescent properties of semen, serum, urine, saliva and fingermarks over time were investigated, by means of fluorescence spectroscopy, to identify specific fluorescent signatures for body fluid identification. The samples were excited at 81 different excitation wavelengths ranging from 200 to 600 nm and for each excitation wavelength the emission was recorded between 220 and 700 nm. Subsequently, the total emitted fluorescence intensities of specific fluorescent signatures in the UV–visible range were summed and principal component analysis was performed to cluster the body fluids. Three combinations of four principal components allowed specific clustering of the body fluids, except for fingermarks. Blind testing showed that 71.4% of the unknown samples could be correctly identified. This pilot study shows that the fluorescent behavior of ageing body fluids can be used as a new non-invasive tool for body fluid identification, which can improve the current guidelines for the detection of body fluids in forensic practice and provide the robustness of methods that rely on fluorescence.

Detection of morphotropic phase boundary in A-site/Ca-substituted Na0. 5Bi0. 5TiO3 complex oxides ferroelectric system

Vibrational and structural properties of lead-free piezoelectric (1-x)Na0.5Bi0.5TiO3–xCaTiO3 (0 < x < 1.00) solid solutions have been investigated using Raman spectroscopy and X-ray diffraction. Different anomalies were detected and analyzed taking into consideration the phase transition from rhombohedral to orthorhombic phase at room temperature. All Raman bands were interpreted through the variation in the peak positions (frequency) and the corresponding half-widths at half maximum (HWHM) as a function of x. XRD used as a complementary technique to Raman spectroscopy, showed that the rhombohedral – orthorhombic phase transition went gradually through an intermediate phase consisting of a mixture of rhombohedral (R3c) and orthorhombic (Pnma) structures and that the fraction of orthorhombic phase increased with CT composition. The results show that the morphotropic phase boundary (MPB) is located between 0.09 and 0.15.