Background: To assess the prevalence of chronic lymphedema and trismus in patients > 6 months after head and neck cancer (HNC) treatment, and to explore how the severity of these conditions correlates with body image and quality of life. Methods: The cross-sectional sample included 59 patients, treated for HNC between six months to three years ago. Physical measurements were performed to assess the presence of external lymphedema and trismus (<36 mm). Furthermore, participants completed two questionnaires regarding body image (BIS) and quality of life (UW-QoL V4). Results: Lymphedema prevalence was 94.1% (95% CI 0.86–0.98), with a median severity score of 9 (range 0–24). Trismus prevalence in this sample was 1.2%. The median BIS score was 2, indicating a positive body image. The UW-QoL score showed a good QOL with a median of 100. Only the domain of saliva and overall related health had a lower median of 70 and 60, respectively. There was no correlation between lymphedema and body image (r = 0.08, p = 0.544). Patients with higher lymphedema scores reported poorer speech with a moderate correlation (r = −0.39, p = 0.003). Conclusion: Lymphedema is a highly prevalent, but moderately severe late side-effect of HNC with a limited impact on quality of life domains except for speech, in our cohort.
Non-invasive, rapid, on-site detection and identification of body fluids is highly desired in forensic investigations. The use of fluorescence-based methods for body fluid identification, have so far remain relatively unexplored. As such, the fluorescent properties of semen, serum, urine, saliva and fingermarks over time were investigated, by means of fluorescence spectroscopy, to identify specific fluorescent signatures for body fluid identification. The samples were excited at 81 different excitation wavelengths ranging from 200 to 600 nm and for each excitation wavelength the emission was recorded between 220 and 700 nm. Subsequently, the total emitted fluorescence intensities of specific fluorescent signatures in the UV–visible range were summed and principal component analysis was performed to cluster the body fluids. Three combinations of four principal components allowed specific clustering of the body fluids, except for fingermarks. Blind testing showed that 71.4% of the unknown samples could be correctly identified. This pilot study shows that the fluorescent behavior of ageing body fluids can be used as a new non-invasive tool for body fluid identification, which can improve the current guidelines for the detection of body fluids in forensic practice and provide the robustness of methods that rely on fluorescence.
MULTIFILE
Background & Aim:Technological innovations enable rapid DNA analysis implementation possibilities. Concordantly, rapid DNA devices are being used in practice. However, the effects of implementing rapid DNA technologies in the crime scene investigation procedure have only been evaluated to a limited extent.In this study, the rapid DNA procedure at the crime scene was compared with a regular DNA procedure. We investigated the DNA-results of the RapidHIT200 on blood and saliva traces secured at the crime scene, and the impact of these rapid DNA profile results on the investigative process & DNA Hits.
The continuous monitoring of health indicators in biofluids such as sweat, saliva, blood, and urine has great potential for preventive medicine. Techniques that continuously monitor biomarkers still remain a major technological challenge. Recently, a concept of dynamic biosensing was published that is based on mediator particles. Such mediator particles exhibit rapid switching between a bound and unbound state during interaction with a probing structure to which they are connected through a molecular tether (like a balloon on a string). Although the concept of using mediator particles for dynamics biosensing is very promising, the used detection method is not a viable solution as it is not miniaturizable. We propose to use a photonic ring resonator (RR) or Mach-Zender interferometer (MZI) as the probing structure in combination with a highly miniaturizable readout scheme. In this project, we perform preliminary experiments to prove that this photonic approach can be used for the detection of the mediator particles tethered to the photonic waveguide. To bridge the gap with the practical application by health professionals, we will enrich the envisioned solution through OnePlanet's OpenEd program. OpenEd aims to share technology and innovations (e.g. prototypes) with educational institutes (MBO, HBO) that want to further innovate their courses or work methods, such that current and future professionals are well prepared to work with new (digital) technologies. By presenting our use-case as a 'challenge' to teachers, students and practitioners, OpenEd also allows enriching the use-case by involving (future) health professionals that can provide feedback on - or further investigation of - the practical application of our new technology from the health professional's perspective.
