Thermal injury destroys the physical skin barrier that normally prevents invasion of microorganisms. This and concomitant depression of local and systemic host cellular and humoral immune responses are important factors that contribute to colonization and infection of the burn wound. One of the most common burn wound pathogens is Staphylococcus aureus. Staphylococcus aureus is both a human commensal and a frequent cause of infections leading to mild to life-threatening diseases. Despite a variety of infection control measures, for example patient cohorting and contact precaution at burn centres, S. aureus is still frequently encountered in burn wounds. Colonization with S. aureus has been associated with delayed wound healing, increased need for surgical interventions, and prolonged length of stay at burn centres. In this minireview, we focus on S. aureus nasal carriage in relation to S. aureus burn wound colonization and subsequent infection, and its impact on strategies for infection control. © 2009 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd.
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To accelerate differentiation between Staphylococcus aureus and Coagulase Negative Staphylococci (CNS), this study aimed to compare six different DNA extraction methods from 2 commonly used blood culture materials, i.e. BACTEC and Bact/ALERT. Furthermore, we analyzed the effect of reduced blood culture times for detection of Staphylococci directly from blood culture material. A real-time PCR duplex assay was used to compare 6 different DNA isolation protocols on two different blood culture systems. Negative blood culture material was spiked with MRSA. Bacterial DNA was isolated with: automated extractor EasyMAG (3 protocols), automated extractor MagNA Pure LC (LC Microbiology Kit MGrade), a manual kit MolYsis Plus, and a combination between MolYsis Plus and the EasyMAG. The most optimal isolation method was used to evaluate reduced bacterial culture times. Bacterial DNA isolation with the MolYsis Plus kit in combination with the specific B protocol on the EasyMAG resulted in the most sensitive detection of S.aureus, with a detection limit of 10 CFU/ml, in Bact/ALERT material, whereas using BACTEC resulted in a detection limit of 100 CFU/ml. An initial S.aureus load of 1 CFU/ml blood can be detected after 5 hours of culture in Bact/ALERT3D by combining the sensitive isolation method and the tuf LightCycler assay.
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This paper describes the participatory development process of a web-based communication system focusing on disease management, particularly infection control of Methicillin-resistant Staphylococcus aureus (MRSA). These infections are becoming a major public health issue; they can have serious consequences such as pneumonia, sepsis or death [1]. This makes it even more important for people to be provided with up-to-date and reliable information. Users of a bilingual communication system (Dutch and German) participated in the development process via a needs assessment, the co-creation of the content and the system via usability tests, and in the summative evaluation of the usage of the system. The system enabled users to search efficiently and effectively for practical and relevant information. Moreover, we found that the participation of the intended users is a prerequisite to create a fit between the needs and expectations of the end-users, the technology and the social context of usage of technology. The summative evaluation showed that the system was frequently used (approximately 11,000 unique visitors per month). The most popular categories include ‘MRSA in general’ (20%, both languages) and ‘Acquiring MRSA’ (17% NL, 13% GER). Most users enter the site using internet search engines (Google) instead of the on-site search engine. When they are on the site, they prefer convenient searching via FAQ or related questions. Furthermore, the results showed that the participation of stakeholders is a prerequisite for a successful implementation of the system. To guide the participation of stakeholders we developed a roadmap that integrates human-centered development with business modelling activities.
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Bij de huidaandoeningen acne en rosacea komen ontstekingen van de huid voor. Patiënten krijgen dan vaak antibiotica voorgeschreven. Bacteriën kunnen uiteindelijk resistent worden tegen deze middelen. Met de AROSA-studie willen we het gebruik van antibiotica verminderen.Doel We willen inzicht krijgen in de rol die de huidbacterie Staphylococcus aureus speelt bij acne en rosacea. Daarnaast onderzoeken we of endolysine-therapie de klachten bij acne en rosacea kan verminderen. We hopen dat daardoor een gerichtere diagnose en behandeling mogelijk is en we daarmee het gebruik van antibiotica verminderen. Resultaten Dit onderzoek loopt nog. Na afronding vind je hier een samenvatting van de resultaten. Looptijd 01 maart 2018 - 31 december 2020 Aanpak We onderzoeken een veelbelovende therapie op basis van endolysines. De huidbacterie Staphylococcus aureus kan huidklachten bij acne en rosacea verergeren. De therapie pakt specifiek deze bacterie aan en wekt geen resistentie op. Hogeschool Utrecht, opleiding Huidtherapie heeft meegewerkt aan een wetenschappelijke documentaire over antibiotica-resistentie. Deze resistentie is een groeiend probleem, ook bij de huidaandoeningen acne en rosacea. In de documentaire is te zien hoe een behandeling op basis van endolysines mogelijk kan worden ingezet bij de behandeling van huidaandoeningen. Bekijk de documentaire. Heb je interesse of vragen? Mail naar polikliniekhuidtherapie@hu.nl
