This study aimed to evaluate technological (acidification, proteolysis, lipolysis, resistance to low pH, NaCl, and bile salts) and biopreservation (antimicrobial activity against foodborne pathogens) features of 1002 LAB by high throughput screening (HTS) methods. The LAB was isolated from 11 types of Brazilian artisanal cheeses (BAC) marketed in the main 5 producing regions. Remarkable intra-species variability in acidification rates have been found, which was most pronounced between isolates from Mina's artisanal cheeses, Caipira and Coalho cheeses. Lacticaseibacillus paracasei and Levilactobacillus brevis showed the fastest acidification rate; however, all isolates showed slower acidification rates than a lactococcal control strain (4.3 × lower). When testing inhibitory effects, > 75% of LAB isolates could inhibit the growth of Staphylococcus aureus ATCC 19095 and Listeria monocytogenes ATCC 7644. Two of these isolates, identified as Lactiplantibacillus plantarum and Lentilactobacillus buchneri, the sterile and neutral supernatants alone, were sufficient to inhibit L. monocytogenes growth. Principal component analysis (PCA) allowed the identification of functional groups based on proteolytic and lipolytic activity, osmotic stress resistance, and inhibition of L. monocytogenes. The type of cheese the isolates were recovered from influenced properties such as anti-listerial compounds and lipolytic enzyme production. The use of HTS and multivariate statistics allowed insights into a diverse set of LAB technological and biopreservation properties. These findings allow a profound knowledge of the heterogeneity of a large set of isolates, which can be further used to design starter cultures with varied and combined properties, such as biopreservation and technological features. Besides that, HTS makes it possible to analyze a vast panel of LAB strains, reducing costs and time within laboratory analysis, while avoiding the loss of information once all LAB are tested at the same time (differently from the traditional labor-intensive approach, in which a few numbers of strains is tested per time).
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Growth conditions have been frequently studied in optimizing polyhydroxybutyrate (PHB) production, while few studies were performed to unravel the dynamic mixed microbial consortia (MMCs) in the process. In this study, the relationship between growth conditions (C/N ratios and pH) and the corresponding key-microbes were identified and monitored during PHB accumulation. The highest PHB level (70 wt% of dry cell mass) was obtained at pH 9, C/N 40, and acetic acid 10 g/L. Linking the dominant genera with the highest point of PHB accumulation, Thauera was the most prevalent species in all MMCs of pH 9, except when a C/N ratio of 1 was applied. Notably, dominant bacteria shifted at pH 7 (C/N 10) from Thauera (0 h) to Paracoccus, and subsequently to Alcaligenes following the process of PHB accumulation and consumption. Further understanding of the relationship between the structure of the microbial community and the performance will be beneficial for regulating and obtaining high PHB accumulation within an MMC. Our study illustrates the impact of C/N ratios and pH on microbial prevalence and PHB production levels using a mixed microbial starter culture. This knowledge will broaden industrial perspectives for regulating high PHB production and timely harvesting.
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In the production of fermented foods, microbes play an important role. Optimization of fermentation processes or starter culture production traditionally was a trial-and-error approach inspired by expert knowledge of the fermentation process. Current developments in high-throughput 'omics' technologies allow developing more rational approaches to improve fermentation processes both from the food functionality as well as from the food safety perspective. Here, the authors thematically review typical bioinformatics techniques and approaches to improve various aspects of the microbial production of fermented food products and food safety.
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Dairy products are known for their relatively low impact on the environment per unit of nutritional value. The carbon footprint of cheese from the Netherlands has been reduced in recent years by minimizing energy and water consumption. However,there are other options for further improving the sustainability of cheese production. The dairy research team at Van Hall Larenstein University of Applied Sciences is revealing new possibilities.
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The Hague University of Applied Sciences has high ambitions in the field of internationalisation. Two out of four priorities in the institutional policy touch this theme: global citizenship and internationalisation. In order to ensure that the curriculum of the new degree programme HBO ICT meets these priorities, it is interesting to know which international competencies the ICT sector requires. The main research questions in this report is: Which international competencies does the ICT sector demand of ICT graduates and how can these be embedded in the curriculum of the new HBO ICT degree programme? That the question is relevant, is shown by the fact that 25% of the respondents, ICT graduates, indicated that they actually work abroad for longer and shorter periods. In this research an online survey was held among alumni (n = 315) of the precursors of the HBO ICT degree programme in order to find out which international competencies are important. By conducting interviews on the same target group, this information was deepened. In an online survey among graduation supervisors (n = 202) it is examined to what extent the graduates master the required skills by the end of their training. This combined information provides the input to develop the new curriculum of the HBO ICT degree programme and its specialisations. The results show that English and especially English listening and reading skills are considered to be very important. Our alumni master these skills highly satisfactorily. It was specifically mentioned, however, that alumni must overcome a certain reluctance to speak. Intercultural and personal and social competencies are found very important. To master these competencies, students should learn by experiencing. This can be done by working together in international teams, but also in national teams as long as they are supervised explicitly on intercultural, personal and social competencies. As far as the international academic and professional competencies concerned, especially internationally accepted professional knowledge is considered important. On these categories the HBO ICT graduates score satisfactorily (a score of 6 or 6,5 out of 10). Depending on the ambitions of the programme, some improvements could be made here. In general, the ICT sector is quite satisfied with the extent to which our students possess international competencies they consider to be relevant. However, there are suggestions for improvement and some of them have already been included in the toolkit internationalisation as part of the development of the curriculum of HBO ICT.
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Bundeling van vijf essays, geschreven door hbo-studenten Social Work. Zij volgden in studiejaar 2023/2024 een half jaar de minor Inclusiviteit in de stad aan de Haagse Hogeschool. In die minor zoomen we in op hedendaagse uitsluitingsmechanismen die bepaalde groepen in onze samenleving extra hard treffen. Het centrale thema van de essays is inclusiviteit. In elk essay is te zien hoe belangrijk dit onderwerp is voor de nieuwe generatie. De auteurs gaan in op de verschillende dimensies van inclusiviteit, variërend van uitsluiting door armoede, door culturele verschillen maar ook door het kiezen van het beroep sekswerk. Uitsluiting heeft een diep effect op het welzijn van mensen, op toegankelijkheid tot instituties en op sociale rechtvaardigheid.
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For environmental governance to be more effective and transformative, it needs to enhance the presence of experimental and innovative approaches for participation. This enhancement requires a transformation of environmental governance, as too often the (public) participation process is set up as a formal obligation in the development of a proposed intervention. This article, in search of alternatives, and in support of this transformation elaborates on spaces where participatory and deliberative governance processes have been deployed. Experiences with two mediated participation methodologies – community art and visual problem appraisal – allow a demonstration of their potential, relevance and attractiveness. Additionally, the article analyzes the challenges that result from the nature of these arts-based methodologies, from the confrontational aspects of voices overlooked in conventional approaches, and from the need to rethink professionals’ competences. Considering current environmental urgencies, mediated participation and social imaginaries still demonstrate capacities to open new avenues for action and reflection.
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This paper investigates whether students change their entrepreneurial entry preference if they are presented with different options. We propose that students’ entry preferences are mediated by concepts proposed by threshold theory: choice options, opportunity costs and psychic income. This study is exploratory in nature, analyzing a small sample of 31 student essays both quantitatively and qualitatively to test our propositions. Though lacking a control group, enrolment in a six-week module on entry mode options by a group of third year Bachelor students at a Dutch university resulted in some interesting changes—in particular, toward greater clarity in the entrepreneurial entry mode preference as well as a shift toward takeover options (including firm acquisition and family succession). However, thematic analysis of students essays reveals that the perceived ability to act on such preferences may still be limited by opportunity costs (i.e., the higher need for financial capital) and a self-perceived lack of human capital (entrepreneurial or management experience).
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The research group ‘Community resilience, participation and social learning’ of theprofessorship Sustainable River Management at Van Hall Larenstein University of Applied Sciences, in the Netherlands explores the contribution of community art and visual arts in relation to complex public participation processes in a context of sustainability.
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