This CIENS-report sums up the main findings from the project “Cultural heritage and water management in urban planning” (Urban WATCH), financed by the Research Council of Norway through the MILJØ2015 programme, and cofunded by the Directorate for Cultural Heritage in Norway (Riksantikvaren) and the Geological Survey of Norway (NGU). The project started up in 2012 and ended in 2015.
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Manure application can spread antimicrobial resistance (AMR) from manure to soil and surface water. This study evaluated the role of the soil texture on the dynamics of antimicrobial resistance genes (ARGs) in soils and surrounding surface waters. Six dairy farms with distinct soil textures (clay, sand, and peat) were sampled at different time points after the application of manure, and three representative ARGs sul1, erm(B), and tet(W) were quantified with qPCR. Manuring initially increased levels of erm(B) by 1.5 ± 0.5 log copies/kg of soil and tet(W) by 0.8 ± 0.4 log copies/kg across soil textures, after which levels gradually declined. In surface waters from clay environments, regardless of the ARG, the gene levels initially increased by 2.6 ± 1.6 log copies/L, after which levels gradually declined. The gene decay in soils was strongly dependent on the type of ARG (erm(B) < tet(W) < sul1; half-lives of 7, 11, and 75 days, respectively), while in water, the decay was primarily dependent on the soil texture adjacent to the sampled surface water (clay < peat < sand; half-lives of 2, 6, and 10 days, respectively). Finally, recovery of ARG levels was predicted after 29–42 days. The results thus showed that there was not a complete restoration of ARGs in soils between rounds of manure application. In conclusion, this study demonstrates that rather than showing similar dynamics of decay, factors such as the type of ARG and soil texture drive the ARG persistence in the environment.
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Climate change and urbanization will increase the frequency and magnitude of urban flooding and water quality problems in many regions of the world. In coastal and delta areas like The Netherlands and the Philippines, where urbanization is often high, there has been an increase in the adoption of sustainable urban drainage systems (SUDS). SUDS are installed around the world with the expectation to reduce urban flooding and reduce the pollution impact on receiving waters. Most cities in Asia are starting to implement SUDS as their strategy to make their cities sustainable and resilient.The combination of SUDS with appropriate wastewater treatment and management systems have the potential to be multifunctional in alleviating flood run-off, improving water quality, alleviating heat stress and as a source for reusing the stormwater and wastewater.Since the earliest SUDS are implemented in Europe decades ago it is advised to use the lessons learnt in this process. International knowledge exchange is promoted in projects as IWASTO where several organisations from the Philippines and The Netherlands join forces on a specific region as the Pateros riverin Manila with the aim to minimise the pollution impact on this receiving water. The first findings of this project related to storm water and wastewater management are presented in this paper. In this stage of the project high level support models that map the challenges in the city (such as flooding and heatstress) arevaluable tools for implementing cost effective sustainable drainage for improving water quality.
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Application of animal manure to soils results in the introduction of manure-derived bacteria and their antimicrobial resistance genes (ARGs) into soils. ResCap is a novel targeted-metagenomic approach that allows the detection of minority components of the resistome gene pool without the cost-prohibitive coverage depths and can provide a valuable tool to study the spread of antimicrobial resistance (AMR) in the environment. We used high-throughput sequencing and qPCR for 16S rRNA gene fragments as well as ResCap to explore the dynamics of bacteria, and ARGs introduced to soils and adjacent water ditches, both at community and individual scale, over a period of three weeks. The soil bacteriome and resistome showed strong resilience to the input of manure, as manuring did not impact the overall structure of the bacteriome, and its effects on the resistome were transient. Initially, manure application resulted in a substantial increase of ARGs in soils and adjacent waters, while not affecting the overall bacterial community composition. Still, specific families increased after manure application, either through the input of manure (e.g., Dysgonomonadaceae) or through enrichment after manuring (e.g., Pseudomonadaceae). Depending on the type of ARG, manure application resulted mostly in an increase (e.g., aph(6)-Id), but occasionally also in a decrease (e.g., dfrB3) of the absolute abundance of ARG clusters (FPKM/kg or L). This study shows that the structures of the bacteriome and resistome are shaped by different factors, where the bacterial community composition could not explain the changes in ARG diversity or abundances. Also, it highlights the potential of applying targeted metagenomic techniques, such as ResCap, to study the fate of AMR in the environment.
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Recent years have seen a global rise in the failure of tailings dams. Studies investigating the causes of slope failure often recognise high intensity rainfall events to significantly contribute to liquefaction, erosion and overtopping. This study aims to investigate the influence of alternative physical and geohydrological processes that, under tension saturation conditions, contribute to slope instability in tailings dams. It has been suggested that the generation of transient pressure wave mechanisms by high intensity rainfall events, surface ponding and wetting front advancement result in the formation of an induced pressure head that triggers the mobilization of pre-event water. In order to quantify these physical processes, this study included the analysis of rapid transmission conditions in a silica fines mix, with similar physical and hydraulic characteristics as platinum tailings. A tall leak-proof soil column, containing the soil sample compacted to in-situ dry bulk density, was fitted with seven observation ports. Each port consisted of a pore air pressure probe, a mini tensiometer and a time domain reflectometry probe. After set-up and initial stabilisation, three separate artificial high intensity rainfall events were applied to the surface. Monitoring of hydraulic state variables was recorded at thirty second intervals by automatic logging, thereby enabling the analysis of measured outcomes. Observations showed instant spikes in pore air pressure ahead of the wetting front, as well as a number of delayed responses. The interpretation of lab results led to the conclusion that pressure diffusion mechanisms throughout the porous medium, could result in the rapid release and mobilisation of previously stagnant antecedent moisture, thereby enabling phreatic levels to rising rapidly and in excess to the amount of surface infiltration. Also, since an increase in pore water pressure is likely to cause a reduction in shear strength, it is suggested that these physical and geohydrological processes could have an adverse impact on the stability of tailings dams.
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Antibiotics are a factor in developing antibiotic resistance in the environment. Outbreaks due to pathogens and resistant bacteria are an emerging issue in this decade. Resistance of Escherichia coli to two groups of antibiotics has been revised recently by the World Health Organization (WHO). These data showed that bacteria have already developed resistance to third and fourth group of antibiotics. The WHO report on surveillance and antibiotics consumption evaluation showed that antibiotic consumption varies in EU countries. Outbreaks have increased in parallel to these data depending on country, season, sex, and age group. This chapter revises the routes of spreading and surveillance of E. coli. There is a particular focus on water sources including hospitals, urban wastewater treatment plants (UWTPs), diffuse sources, and water reuse. Extensively revised data are given on the control techniques by biological and advanced processes. The emerging issue of gene transfer control in parallel to the control of bacteria is expressed. A detailed literature survey of emerging technologies of photocatalysis and nanoparticles is given.
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There is an urgency for developing methods that are capable of monitoring watersystems that are fast changing due to climate change and increase of anthropogenic pressure. Updated and real-time detailed data is necessary to support water and soil management strategies. This study evaluates the implementations of novel techniques in different socio-economic settings. Sensors and cameras were installed in mobile platforms (including boats and underwater drones), and deployed to assess spatial data variability. Environmental scans were performed at multiple locations with different water systems in The Netherlands, Indonesia and Denmark. Results from themultiple methods (sensor, cameras) provided new insights into spatial variation of water quality, contrasting with traditional point sampling. Feedback from waterauthorities and other stakeholders indicate that collected data can be used tosupport management actions, and that such increasingly accessible technologiescontribute to creating awareness to water related issues.
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The impact of the construction industry on the natural environment is severe, natural areas are changedinto predominantly hard solid surfaces, the energy use in the built environment is high and the industryputs huge claims on materials.
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VHL University of Applied Sciences (VHL) is a sustainable University of AppliedSciences that trains students to be ambitious, innovative professionals andcarries out applied research to make a significant contribution to asustainable world. Together with partners from the field, they contribute to innovative and sustainable developments through research and knowledge valorisation. Their focus is on circular agriculture, water, healthy food & nutrition, soil and biodiversity – themes that are developed within research lines in the variousapplied research groups. These themes address the challenges that are part ofthe international sustainability agenda for 2030: the sustainable developmentgoals (SDGs). This booklet contains fascinating and representative examplesof projects – completed or ongoing, from home and abroad – that are linked tothe SDGs. The project results contribute not only to the SDGs but to their teaching as well.
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Stormwater runoff has severe negative and direct impact on the quality of surface waters and groundwater. The impact can cause chemical and heavy-metal pollution. Applying well established methods to map pollutants in urban areas and specifically in Nature-Based Solutions (NBS), such as Sustainable UrbanDrainage Systems (SuDS) is a step towards improving the water quality in the urban water cycle. Traditional mapping of pollutants by the means of soil samples is costly, which is the main reason why the environmental-technical functioning of rainwater facilities has not been investigated on a large scale andsystematically. X-ray fluorescence (XRF) is a known analysing method for finding metals and other components, for laboratory analysis and portable instruments. In this work we propose a new approach of mapping method for pollutants in-situ, such as heavy metals in soil in SuDS, with case studies from theNetherlands where swales were implemented 20 years ago. In situ XRF measurements is a quick and costefficient analysis for heavy meatal mapping in the respect to contaminated soil. In situ XRF measures of various elements, including heavy metals is carried out in a quickscan and accurate manner and measures both qualitatively and quantitatively. It makes the time-consuming and costly interim analyses by laboratories superfluous. In this study, we suggest a new methodology approach for in situ mapping of pollutants in various swales that were implemented from 20 to 5 years ago. The results differ due to multiple factors (age, use of materials, storage volume, maintenance, run off quality, etc.). Several locations reached unacceptable levels, above the national thresholds for pollutants. The spatial distribution of pollutants in the over 30 swales mapped in the Netherlands show that the preferred water flow in theSuDS controls the spreading of pollutants. The swales investigated are presented in an interactive way with the open source tool www.climatescan.nl, containing more than 100 swales, part of which has been investigated with in situ XRF measurements. The research results are of great importance for all stakeholders in (inter)national cities that are involved in climate adaptation. SuDS is the most widely used method for storing stormwater and infiltrating in the Netherlands. However, there is still too little knowledge about the long-term functioning of the soil of these facilities.
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