The increased cultivation of highly productive C4 crop plants may contribute to a second green revolution in agriculture. However, the regulation of mineral nutrition is rather poorly understood in C4 plants. To understand the impact of C4 photosynthesis on the regulation of sulfate uptake by the root and sulfate assimilation into cysteine at the whole plant level, seedlings of the monocot C4 plant maize (Zea mays) were exposed to a non-toxic level of 1.0 µl l−1 atmospheric H2S at sulfate-sufficient and sulfate-deprived conditions. Sulfate deprivation not only affected growth and the levels of sulfur- and nitrogen-containing compounds, but it also enhanced the expression and activity of the sulfate transporters in the root and the expression and activity of APS reductase (APR) in the root and shoot. H2S exposure alleviated the establishment of sulfur deprivation symptoms and seedlings switched, at least partly, from sulfate to H2S as sulfur source. Moreover, H2S exposure resulted in a downregulation of the expression and activity of APR in both shoot and root, though it hardly affected that of the sulfate transporters in the root. These results indicate that maize seedlings respond similarly to sulfate deprivation and atmospheric H2S exposure as C3 monocots, implying that C4 photosynthesis in maize is not associated with a distinct whole plant regulation of sulfate uptake and assimilation into cysteine.
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Publicatie bij de rede, uitgesproken bij de aanvaarding van het ambt als lector Green Biotechnology aan Hogeschool Inholland te Amsterdam op 20 mei2015 door dr. C.M. Kreike
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This paper focuses on utilizing the Celciushouse as an escape room in energy education. In a broader context, it also addresses the incorporation of serious gaming in education. The project is part of COVE SEED. SEED - Sustainable Energy Education, aims to develop innovative vocational education and training, working with experts from five different European regions to phase out fossil fuels and contributing to Europe becoming a fossil free energy continent. SEED is a CoVE (Centres of Vocational Excellence) programme. CoVE’s are part of the Erasmus+ program aiming to establish transnational platforms on, among others, regional development, innovation and inclusion. SEED combines education on various international levels including level 2,3,4, and 6. At this moment, the project ESCAPEROOM IN ENERGY EDUCATION is still in its initial phase. With this paper and the accompanying workshop, we aim to gather insights from other international regions involved in the SEED project collaboration. The acceleration of technological developments means that what is learned today may be outdated tomorrow. Therefore, it is essential for educational institutions to focus on developing general skills such as critical thinking, problem-solving, and the ability to quickly absorb new information. The market demands professionals with modern knowledge and skills. Techniques taught to students today may become outdated tomorrow. Therefore, the ability to learn how to learn is becoming increasingly crucial. Analytical and research skills are therefore gaining importance. It is also essential for students to utilize various learning methods. Not just learning from books but particularly learning from practical experience. Practice-oriented learning, where students gain direct experience in real situations, not only reinforces theoretical knowledge but also develops practical skills that are valuable in the job market. To tackle these problems, serious gaming or the establishment of escape rooms can be a solution.
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