The transition to a biobased economy necessitates utilizing renewable resources as a sustainable alternative to traditional fossil fuels. Bioconversion is a way to produce many green chemicals from renewables, e.g., biopolymers like PHAs. However, fermentation and bioconversion processes mostly rely on expensive, and highly refined pure substrates. The utilization of crude fractions from biorefineries, especially herbaceous lignocellulosic feedstocks, could significantly reduce costs. This presentation shows the microbial production of PHA from such a crude stream by a wild-type thermophilic bacterium Schlegelella thermodepolymerans [1]. Specifically, it uses crude xylose-rich fractions derived from a newly developed biorefinery process for grassy biomasses (the ALACEN process). This new stepwise mild flow-through biorefinery approach for grassy lignocellulosic biomass allows the production of various fractions: a fraction containing esterified aromatics, a monomeric xylose-rich stream, a glucose fraction, and a native-like lignin residue [2]. The crude xylose-rich fraction was free of fermentation-inhibiting compounds meaning that the bacterium S.thermodepolymerans could effectively use it for the production of one type of PHA, polyhydroxybutyrate. Almost 90% of the xylose in the refined wheat straw fraction was metabolized with simultaneous production of PHA, matching 90% of the PHA production per gram of sugars, comparable to PHA yields from commercially available xylose. In addition to xylose, S. thermodepolymerans converted oligosaccharides with a xylose backbone (xylans) into fermentable xylose, and subsequently utilized the xylose as a source for PHA production. Since the xylose-rich hydrolysates from the ALACEN process also contain some oligomeric xylose and minor hemicellulose-derived sugars, optimal valorization of the C5-fractions derived from the refinery process can be obtained using S. thermodepolymerans. This opens the way for further exploration of PHA production from C5-fractions out of a variety of herbaceous lignocellulosic biomasses using the ALACEN process combined with S. thermodepolymerans. Overall, the innovative utilization of renewable resources in fermentation technology, as shown herein, makes a solid contribution to the transition to a biobased economy.[1] W. Zhou, D.I. Colpa, H. Permentier, R.A. Offringa, L. Rohrbach, G.J.W. Euverink, J. Krooneman. Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans. Resources, Conservation and Recycling 194 (2023) 107006, ISSN 0921-3449, https://doi.org/10.1016/j.resconrec.2023.107006. [2] S. Bertran-Llorens, W.Zhou. M.A.Palazzo, D.I.Colpa, G.J.W.Euverink, J.Krooneman, P.J.Deuss. ALACEN: a holistic herbaceous biomass fractionation process attaining a xylose-rich stream for direct microbial conversion to bioplastics. Submitted 2023.
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For the future circular economy, renewable carbon feedstocks manifest considerable promise for synthesizing sustainable and biodegradable polyhydroxyalkanoate (PHA). In this study, 16 wt% and 30 wt% PHA (cell dry weight) are respectively produced by thermophilic Caldimonas thermodepolymerans from beechwood xylan and wheat arabinoxylan as the sole carbon source. Moreover, an in silico study of the potential xylan-degrading proteins was conducted using proteome sequencing and CAZyme specialized bioinformatic tools. This study demonstrates the feasibility of utilizing complex polysaccharide substrates for PHA biosynthesis, thereby potentially eliminate additional processing steps and reducing overall production costs for sustainable plastic.
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Voor de transitie naar een biobased economie is meer nodig dan een goed idee. Het Kenniscentrum Biobased Economy van de Hanzehogeschool Groningen richt zich daarom met bedrijfsleven en partners op wat verder nodig is om innovatieve oplossingen succesvol naar de markt te brengen: ketenmanagement en -kennis, een verandering in interne bedrijfsvoering en een herdefiniëring van waarde en prijs.
The postdoc candidate, Giuliana Scuderi, will strengthen the connection between the research group Biobased Buildings (BB), (collaboration between Avans University of Applied Sciences and HZ University of Applied Sciences (HZ), and the Civil Engineering bachelor programme (CE) of HZ. The proposed research aims at deepening the knowledge about the mechanical properties of biobased materials for the application in the structural and infrastructural sectors. The research is relevant for the professional field, which is looking for safe and sustainable alternatives to traditional building materials (such as lignin asphalt, biobased panels for bridge constructions, etc.). The study of the mechanical behaviour of traditional materials (such as concrete and steel) is already part of the CE curriculum, but the ambition of this postdoc is that also BB principles are applied and visible. Therefore, from the first year of the programme, the postdoc will develop a biobased material science line and will facilitate applied research experiences for students, in collaboration with engineering and architectural companies, material producers and governmental bodies. Consequently, a new generation of environmentally sensitive civil engineers could be trained, as the labour market requires. The subject is broad and relevant for the future of our built environment, with possible connections with other fields of study, such as Architecture, Engineering, Economics and Chemistry. The project is also relevant for the National Science Agenda (NWA), being a crossover between the routes “Materialen – Made in Holland” and “Circulaire economie en grondstoffenefficiëntie”. The final products will be ready-to-use guidelines for the applications of biobased materials, a portfolio of applications and examples, and a new continuous learning line about biobased material science within the CE curriculum. The postdoc will be mentored and supervised by the Lector of the research group and by the study programme coordinator. The personnel policy and job function series of HZ facilitates the development opportunity.
Broedeieren worden door heel Europa standaard op kartonnen trays, gemaakt van papierpulp, vervoerd. Het aanbod van papierpulp neemt af, wat een verhoging van de prijs van papierpulp als grondstof tot gevolg heeft. Dit is echter niet de enige reden om de trays te innoveren en in te passen in een circulaire economisch profiel. Standaard trays zijn namelijk niet sterk genoeg, ze buigen door bij het verplaatsen en dat kan leiden tot schade aan de broedeieren. Dit heeft tot gevolg dat: 1) er minder eieren per tray worden vervoerd dan gewenst, 2) dat ventilatie van de lucht niet optimaal is, wat de gezondheid van de kuikens ‘to-be’ negatief beïnvloedt en, 3) ongeveer 10% van de broedeieren verloren gaat en er dus financiële schade is. Er is daarom behoefte aan een alternatief. Het versterken van de structuur en de ventilatieverbetering van de eiertrays stelt eisen aan de vervanger van de papierpulp. Het moet sterker zijn dan papierpulp en de vormgeving van de eiertrays moet dusdanig zijn dat voldoende ventilatie plaatsvindt. Bamboevezels met een biobased verbindingsmiddel zou hieraan kunnen voldoen. Concreet doel: De selectie van een betaalbaar bio-based en bio-afbreekbaar(circulair) bindmiddel voor bamboevezels en het maken van prototypen eiertrays voor broedeieren. Het doel van dit project is te onderzoeken welke combinatie van (bamboe) vezels en een biobased verbindingsmiddel (binders) een sterke en zeer lichte structuur oplevert. Het moet de potentie hebben om in bulk geproduceerd te worden, dat wil zeggen miljoenen trays per jaar binnen de EU. Het liefst wordt er gebruik gemaakt van bindmiddel uit afvalstromen. Uiteindelijk doel is de ontwikkeling van een nieuwe eiertray die sterk genoeg is om alle eieren te dragen, beter ventileert en (nice-to-have) eetbaar is voor de kuikentjes die uitkomen. Partners in het project: Bambooder, afdeling Biologie faculteit beta-wetenschappen Universiteit Utrecht Instituut Natuur & Techniek en Instituut Life Sciences & Chemistry, Hogeschool Utrecht.
Aiming for a more sustainable future, biobased materials with improved performance are required. For biobased vinyl polymers, enhancing performance can be achieved by nanostructuring the material, i.e. through the use of well-defined (multi-)block, gradient, graft, comb, etc., copolymer made by controlled radical polymerization (CRP). Dispoltec has developed a new generation of alkoxyamines, which suppress termination and display enhanced end group stability compared to state-of-art CRP. Hence, these alkoxyamines are particularly suited to provide access to such biobased nanostructured materials. In order to produce alkoxyamines in a more environmentally benign and efficient manner, a photo-chemical step is beneficial for the final stage in their synthesis. Photo-flow chemistry as a process intensification technology is proposed, as flow chemistry inherently leads to more efficient reactions. In particular, photo-flow offers the benefit of significantly enhancing reactant concentrations and reducing batch times due to highly improved illumination. The aim of this project is to demonstrate at lab scale the feasibility of producing the new generation of alkoxy-amines via a photo-flow process under industrially relevant conditions regarding concentration, duration and efficiency. To this end, Zuyd University of Applied Sciences (Zuyd), CHemelot Innovation and Learning Labs (CHILL) and Dispoltec BV want to enter into a collaboration by combining the expertise of Dispoltec on alkoxyamines for CRP with those of Zuyd and CHILL on microreactor technology and flow chemistry. Improved access to these alkoxyamines is industrially relevant for initiator manufacturers, as well as producers of biobased vinyl polymers and end-users aiming to enhance performance through nanostructuring biobased materials. In addition, access in this manner is a clear demonstration for the high industrial potential of photo-flow chemistry as sustainable manufacturing tool. Further to that, students and professionals working together at CHILL will be trained in this emerging, industrially relevant and sustainable processing tool.
