People’s stories lie at the heart of this paper – stories about how people with vision and creative energy use the arts to address some of the challenges arising from feelings of isolation and dislocation in society. People have to be given the opportunity to speak, to be listened to, so that their observations and experiences can be fed into the wider debate about shaping the frameworks for ‘next’ practicein learning and development in the arts.
To accelerate differentiation between Staphylococcus aureus and Coagulase Negative Staphylococci (CNS), this study aimed to compare six different DNA extraction methods from 2 commonly used blood culture materials, i.e. BACTEC and Bact/ALERT. Furthermore, we analyzed the effect of reduced blood culture times for detection of Staphylococci directly from blood culture material. A real-time PCR duplex assay was used to compare 6 different DNA isolation protocols on two different blood culture systems. Negative blood culture material was spiked with MRSA. Bacterial DNA was isolated with: automated extractor EasyMAG (3 protocols), automated extractor MagNA Pure LC (LC Microbiology Kit MGrade), a manual kit MolYsis Plus, and a combination between MolYsis Plus and the EasyMAG. The most optimal isolation method was used to evaluate reduced bacterial culture times. Bacterial DNA isolation with the MolYsis Plus kit in combination with the specific B protocol on the EasyMAG resulted in the most sensitive detection of S.aureus, with a detection limit of 10 CFU/ml, in Bact/ALERT material, whereas using BACTEC resulted in a detection limit of 100 CFU/ml. An initial S.aureus load of 1 CFU/ml blood can be detected after 5 hours of culture in Bact/ALERT3D by combining the sensitive isolation method and the tuf LightCycler assay.
Many policy documents addressing the future of teacher education do not take into account the fundamental unpredictability of the future, nor the opposing forces that will try to influence that future. Through the analysis of 48 scenario documents on the future of education or teacher education, we identified a set of unpredictable key factors that have to be taken into account when addressing the future of teacher education. We also identified four main futures that may lie ahead for teacher education. We analyzed these four scenarios using the concepts of activity systems, boundary objects, and boundary crossing. This revealed that the extent to which activity systems are open to boundary crossing and are willing to remove institutional boundaries, will largely define the future that lies ahead for teacher education. Future scenarios in themselves can play a role as boundary objects that facilitate the dialogue and boundary crossing between these activity systems
Digital innovations in the field of immersive Augmented Reality (AR) can be a solution to offer adults who are mentally, physically or financially unable to attend sporting events such as premier league football a stadium and match experience. This allows them to continue to connect with their social networks. In the intended project, AR content will be further developed with the aim of evoking the stadium experience of home matches as much as possible. The extent to which AR enriches the experience is then tested in an experiment, in which the experience of a football match with and without AR enrichment is measured in a stadium setting and in a home setting. The experience is measured with physiological signals. In addition, a subjective experience measure is also being developed and benchmarked (the experience impact score). Societal issueInclusion and health: The joint experience of (top) sports competitions forms a platform for vulnerable adults, with a limited social capital, to build up and maintain the social networks that are so necessary for them. AR to fight against social isolation and loneliness.
The Cashing Cashew project focuses on isolation and purification of Cashew Nut Shell Liquid (CNSL) from Cashew Nut Shells (CNS) in order to fully utilize this valuable by-product of the cashew nut production. Global cashew nut production is about 4 million mt/ tons/yr. Of the cashew nut, about 70 % is shell that is removed in processing and currently typically burned as a dirty and inefficient fuel or discarded as waste. This is not only creating an environmental issue but also wasting valuable by-products. The shell contains circa 20-30 % brown viscous liquid, Cashew Nut Shell Liquid (CNSL). This natural resin contains valuable chemical components, for example, cardanol, cardol, and anacardic acid. CNSL and its derivatives have several industrial uses as for example biobased additives, polymeric building blocks, and biodiesel. Part of the CNSL can be extracted during the roasting process prior to separating the shell and nut kernel. The shell waste still has a high CNSL concentration that can be isolated by solvents or pressing (expeller). Expeller process is simple and not capital-intensive; therefore it is commonly used. The main disadvantages of the method are the high energy consumption and that 3-5 % oil remains in the press-cake producing harmful gases in burning. Also, the resulting cake is too dense to be further processed to charcoal or other useful application. The objective of this project is to study the purification of the CNSL obtained from pyrolytic isolation to find the most efficient way of making use of the CNSL oil and the total Cashew Nut Shell biomass. An initial evaluation of potential applications is also performed.
Former military fortifications are often repurposed for tourism and recreation. While some of over 100 Dutch forts are recognized as UNESCO World Heritage sites, a substantial number are currently underdeveloped, putting their cultural and natural heritage at risk. Developing these forts in a conscious and collaborative way promises to not only preserve their heritage value, but also facilitate enjoyable and healthy experiences for visitors. Moreover, under-developed forts provide an opportunity to solve another pressing challenge, namely overtourism. Visitor pressure at tourist attractions has led the Netherlands Board of Tourism and Conventions to call for spreading visitors to lesser-known areas. Less-developed forts are among the most promising of these. Development initiatives depend on a transition from isolation to cooperation across sites. However, for cooperation to be effective, agencies managing these forts have indicated an urgent need for data on visitor characteristics and experiences. The purpose of the present project is to measure and analyze visitor demographics, motivations, and experiences at less-developed forts, and to develop a toolkit to inspire, support, and monitor development of these forts for natural and cultural heritage preservation and improved visitor experience. This proposal builds on the previous project, “Experiencing Nature” which utilized Breda Experience Lab technologies to measure visitor experiences at Fort de Roovere. We now aim to broaden this proven approach to a broader variety of forts, and to translate visitor data into actionable advice. The consortium includes a changemaking network of the Alliantie Zuiderwaterlinie (NL), Regionale Landschappen (VL), and Agentschap Natuur en Bos (VL). This Dutch-Flemish network aims to connect formerly isolated forts to one another, and represents a broad diversity of fortified sites, each with unique challenges. The project will thus facilitate interregional collaboration, especially toward coming Interreg EU proposals, and inform interregional marketing campaigns and planning for management and conservation.