How well-being changes over the course of a vacation is unclear. Particular understudied areas include the eudaimonic dimension of well-being, the comparison between eudaimonia and hedonia, and the role of activity type. Using an integrated model, two studies which combined survey and experiment were conducted to examine the change patterns of eudaimonia and hedonia, the difference of change patterns between eudaimonia and hedonia, and the moderating role of activity type. Hedonia and eudaimonia both significantly changed via a ‘first rise then fall’ change tendency over the course of a vacation. Compared to hedonia, eudaimonia has lower change intensity over the course of a vacation; eudaimonia achieved in a challenging (vs. relaxing) activity is more. Theoretical and managerial implications are discussed.