Previous work on tourists' positive and negative affect has mainly used cross-sectional data. Consequently, little is known about how motivations are related to tourists' emotions over an extended period of time. The purpose of this study was to understand the impact of travel motivation on tourists' emotions and whether the impact would remain the same across different time points. The sample consisted of a panel of approximately 2000 leisure travelers in the Netherlands. After eliminating missing data, 412 panelists completed all seven questionnaires over the nine months of the study. The results indicated that motivation does not have a significant impact on tourists' emotions over a relatively long period of time. Specifically, the study found that travel motivations or a cluster of travel motivations do not seem to have significant within-subject or between-subject impacts on tourists' emotions over a nine-month period. The findings demonstrate the complex relationships between tourists’ travel motivation and emotions and highlight the importance of a longitudinal approach to studying emotions in the tourism context. Managerial implications for destination marketers are discussed.