The die-off of the long-spined sea urchin Diadema antillarum in the 1980s highlighted its crucial role as a primary grazer in tropical western Atlantic coral reefs. However, natural recovery has been slow, exacerbated by a new die-off in 2022. Interest in actively restoring D. antillarum populations has grown with the emergence of culture and rearing techniques. Restocking reefs with laboratory-reared urchins shows potential for enhancing coral reef resilience by reducing algal cover and promoting coral settlement, but success rates vary. Predation and migration contribute to low retention rates, with distinguishing between them is challenging. In this study near Saba, Caribbean Netherlands, we released 200 laboratory-reared D. antillarum on a reef and monitored for D. antillarum retention and potential D. antillarum predator presence and interaction for 35 days. Only 40% of the urchins were still present on the reef after day one. The Spanish hogfish Bodianus rufus was identified as the primary daytime predator, responsible for nine direct predation events. No nighttime predation was observed, but interaction with a batwing coral crab Carpilius corallinus was noted. These insights can help optimize future restocking attempts and emphasize the importance of assessing predator presence beforehand. Reefs with high abundances of predators such as Spanish hogfish should be avoided for D antillarum restocking. In addition, before being released, lab-reared animals should be given time to acclimate to conditions in the wild by being placed in protected in situ cages. Here, they could also grow to larger sizes that are less vulnerable to predation.
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We share insights from our practice-based experimentation with ‘feral’ ways of sensemaking in the context of creative transformational practices. Drawing on three art and design research projects, we discuss how feral ways–open-ended, spontaneous, welcoming indeterminacy – may foster more-than-human co-creation of knowledge and data, and nurture shifts from anthropocentric ‘making sense of’ to relational ‘making sense-with’ other-than-human creatures. Through our cases, we illustrate how experimenting with feralness can foreground issues of power, agency, and control in the currently human-centric discourses around data, technology, and sensemaking in eco-social transformation. Our insights may nurture critical more-than-human perspectives in creative eco-social inquiries.
from the article: "Cow's milk-derived whey hydrolysates are milk substitutes for cow's milk allergic infants. Safety assessment of these hydrolysates is crucial. Currently, huFcεRIα-RBL-2H3 cells, sensitized with serum IgE from cow's milk allergic patients, are used to assess in vitro residual allergenicity. However, limited availability and high inter-lot variation of sera impede the standardization of safety testing. Recently, we generated an oligoclonal pool of chimeric human (chu)IgE antibodies against bovine β-lactoglobulin (BLG) as an alternative for human serum. These antibodies demonstrated increased sensitivity, specificity and reproducibility. An inter-laboratory ring trial using our new degranulation assay with different whey-based hydrolysates was performed at four independent laboratories to investigate the robustness and reproducibility. RBL-2H3 cells expressing huFcεRIα were sensitized with our oligoclonal pool of anti-BLG chuIgE antibodies. The cells were subsequently incubated with an amino-acid based formula (AAF), two extensively hydrolyzed formulas (eHF) and three partially hydrolyzed formulas (pHF) to assess the degranulation upon challenge. Results demonstrated a very strong inter-laboratory correlation and the intra- and inter-laboratory variations were acceptable. The AAF and both eHFs showed no degranulation, whereas all pHFs demonstrated degranulation. The study showed that this degranulation assay is robust and reproducible within and between laboratories. This new in vitro degranulation assay seems predictive for allergenicity outcome and might therefore be considered as a relevant substitute for animal models."
An important line of research within the Center of Expertise HAN BioCentre is the development of the nematode Caenorhabditis elegans as an animal testing replacement organism. In the context of this, us and our partners in the research line Elegant! (project number. 2014-01-07PRO) developed reliable test protocols, data analysis strategies and new technology, to determine the expected effects of exposure to specific substances using C. elegans. Two types of effects to be investigated were envisaged, namely: i) testing of possible toxicity of substances to humans; and ii) testing for potential health promotion of substances for humans. An important deliverable was to show that the observed effects in the nematode can indeed be translated into effects in humans. With regard to this aspect, partner Preventimed has conducted research in obesity patients during the past year into the effect of a specific cherry extract that was selected as promising on the basis of the study with C. elegans. This research is currently being completed and a scientific publication will have to be written. The Top Up grant is intended to support the publication of the findings from Elegant! and also to help design experimental protocols that enable students to become acquainted with alternative medical testing systems to reduce the use of laboratory animals during laboratory training.
Cell-based production processes in bioreactors and fermenters need to be carefully monitored due to the complexity of the biological systems and the growth processes of the cells. Critical parameters are identified and monitored over time to guarantee product quality and consistency and to minimize over-processing and batch rejections. Sensors are already available for monitoring parameters such as temperature, glucose, pH, and CO2, but not yet for low-concentration substances like proteins and nucleic acids (DNA). An interesting critical parameter to monitor is host cell DNA (HCD), as it is considered an impurity in the final product (downstream process) and its concentration indicates the cell status (upstream process). The Molecular Biosensing group at the Eindhoven University of Technology and Helia Biomonitoring are developing a sensor for continuous biomarker monitoring, based on Biosensing by Particle Motion. With this consortium, we want to explore whether the sensor is suitable for the continuous measurement of HCD. Therefore, we need to set-up a joint laboratory infrastructure to develop HCD assays. Knowledge of how cells respond to environmental changes and how this is reflected in the DNA concentration profile in the cell medium needs to be explored. This KIEM study will enable us to set the first steps towards continuous HCD sensing from cell culture conditions controlling cell production processes. It eventually generates input for machine learning to be able to automate processes in bioreactors and fermenters e.g. for the production of biopharmaceuticals. The project entails collaboration with new partners and will set a strong basis for subsequent research projects leading to scientific and economic growth, and will also contribute to the human capital agenda.
The European eel (Anguilla anguilla) is a delicacy fish and an integral part of the Dutch culinary history. However, the stock of adult eel has decreased significantly due to a precipitous recruitment of glass eel fall. This relates to multiple factors including obstacles in migration pathways, loss of habitat and chemical pollution. Consequently, Anguilla anguilla has become a critically endangered species and is protected under European legislation. One possible solution, explored on laboratory scale, is the captive reproduction of eels and growth of glass eel in aquaculture. A big challenge of this technique is the limiting aspect of possible nutrients for the eels in the larval stage, as the diet must be delivered in micrometric capsules (< 20 µm) with a high protein content. Such diets are not yet available on the market. Electrohydrodynamic atomization (EHDA) is a novel option to prepare a micro-diet suitable for eel larvae. EHDA is especially interesting for its narrow size distribution capabilities and for applications which require submicrometric sizes. This project aims to evaluate the use of EHDA to produce high protein content micrometric size capsules for feeding larval eels. If successful, this would assist in the captivity production of glass eel and to make the eel culture independent of wild catches, restoring the culinary market. The project will be conducted in two phases. Firstly, tests will be conducted to evaluate the necessary conditions of the capsules using EHDA. Subsequently, the obtained capsules will be tested as feed for eel larvae. The main objective is to favour the development of a more sustainable eel culture, regarding the possibilities of investigating the current fish in natura option and exchanging it for a captivity one.
