During crime scene investigations, numerous traces are secured and may be used as evidence for the evaluation of source and/or activity level propositions. The rapid chemical analysis of a biological trace enables the identification of body fluids and can provide significant donor profiling information, including age, sex, drug abuse, and lifestyle. Such information can be used to provide new leads, exclude from, or restrict the list of possible suspects during the investigative phase. This paper reviews the state-of-the-art labelling techniques to identify the most suitable visual enhancer to be implemented in a lateral flow immunoassay setup for the purpose of trace identification and/or donor profiling. Upon comparison, and with reference to the strengths and limitations of each label, the simplistic one-step analysis of noncompetitive lateral flow immunoassay (LFA) together with the implementation of carbon nanoparticles (CNPs) as visual enhancers is proposed for a sensitive, accurate, and reproducible in situ trace analysis. This approach is versatile and stable over different environmental conditions and external stimuli. The findings of the present comparative analysis may have important implications for future forensic practice. The selection of an appropriate enhancer is crucial for a well-designed LFA that can be implemented at the crime scene for a time- and cost-efficient investigation.
DOCUMENT
From the article: "A facile approach for the fabrication of large-scale interdigitated nanogap electrodes (nanogap IDEs) with a controllable gap was demonstrated with conventional micro-fabrication technology to develop chemocapacitors for gas sensing applications. In this work, interdigitated nanogap electrodes (nanogap IDEs) with gaps from 50–250 nm have been designed and processed at full wafer-scale. These nanogap IDEs were then coated with poly(4-vinyl phenol) as a sensitive layer to form gas sensors for acetone detection at low concentrations. These acetone sensors showed excellent sensing performance with a dynamic range from 1000 ppm to 10 ppm of acetone at room temperature and the observed results are compared with conventional interdigitated microelectrodes according to our previous work. Sensitivity and reproducibility of devices are discussed in detail. Our approach of fabrication of nanogap IDEs together with a simple coating method to apply the sensing layer opens up possibilities to create various nanogap devices in a cost-effective manner for gas sensing applications"
MULTIFILE
The thoracic and peritoneal cavities are lined by serous membranes and are home of the serosal immune system. This immune system fuses innate and adaptive immunity, to maintain local homeostasis and repair local tissue damage, and to cooperate closely with the mucosal immune system. Innate lymphoid cells (ILCs) are found abundantly in the thoracic and peritoneal cavities, and they are crucial in first defense against pathogenic viruses and bacteria. Nanomaterials (NMs) can enter the cavities intentionally for medical purposes, or unintentionally following environmental exposure; subsequent serosal inflammation and cancer (mesothelioma) has gained significant interest. However, reports on adverse effects of NMon ILCs and other components of the serosal immune systemare scarce or even lacking. As ILCs are crucial in the first defense against pathogenic viruses and bacteria, it is possible that serosal exposure to NMmay lead to a reduced resistance against pathogens. Additionally, affected serosal lymphoid tissues and cells may disturb adipose tissue homeostasis. This review aims to provide insight into key effects of NMon the serosal immune system.
DOCUMENT
The growing energy demand and environmental impact of traditional sources highlight the need for sustainable solutions. Hydrogen produced through water electrolysis, is a flexible and clean energy carrier capable of addressing large-electricity storage needs of the renewable but intermittent energy sources. Among various technologies, Proton Exchange Membrane Water Electrolysis (PEMWE) stands out for its efficiency and rapid response, making it ideal for grid stabilization. In its core, PEMWEs are composed of membrane electrode assemblies (MEA), which consist of a proton-conducting membrane sandwiched between two catalyst-coated electrodes, forming a single PEMWE cell unit. Despite the high efficiency and low emissions, a principal drawback of PEMWE is the capital cost due to high loading of precious metal catalysts and protective coatings. Traditional MEA catalyst coating methods are complex, inefficient, and costly to scale. To circumvent these challenges, VSParticle developed a technology for nanoparticle film production using spark ablation, which generates nanoparticles through high-voltage discharges between electrodes followed by an impaction printing module. However, the absence of liquids poses challenges, such as integrating polymeric solutions (e.g., Nafion®) for uniform, thicker catalyst coatings. Electrohydrodynamic atomization (EHDA) stands out as a promising technique thanks to its strong electric fields used to generate micro- and nanometric droplets with a narrow size distribution. Co-axial EHDA, a variation of this technique, utilizes two concentric needles to spray different fluids simultaneously.The ESPRESSO-NANO project combines co-axial EHDA with spark ablation to improve catalyst uniformity and performance at the nanometer scale by integrating electrosprayed ionomer nanoparticles with dry metal nanoparticles, ensuring better distribution of the catalyst within the nanoporous layer. This novel approach streamlines numerous steps in traditional synthesis and electrocatalyst film production which will address material waste and energy consumption, while simultaneously improve the electrochemical efficiency of PEMWEs, offering a sustainable solution to the global energy crisis.
Vanuit het bedrijfsleven is vraag naar het ontwikkelen van coatings met specifieke hoogwaardige eigenschappen. Een technisch haalbare en kosten efficiënte methode om dit te doen is door het inmengen van nanodeeltjes in coatings of in polymeren. Op dit moment is de beschikbaarheid (op grotere schaal) van hoogwaardige nanodeeltjes (grootte en deeltjesgrootte distributie) echter nog een knelpunt. Microreactortechnologie kan hiervoor een goede uitkomst bieden. In een microreactor kunnen reactiecondities zeer goed gecontroleerd worden en daardoor zal de reproduceerbaarheid goed zijn. Ook is het eenvoudig om een reactie in een microreactor op te schalen naar een groter volume. In het RAAK-MKB project Flow4Nano worden 2 sleutel technologieën van het lectoraat Material Sciences van Zuyd Hogeschool bij elkaar gebracht: nanotechnologie en microreactor technologie. In dit project zal de focus liggen op de toepassing van nanodeeltjes in optische coating voor zonnecellen en voor glastuinbouw. De toepassing in zonnecellen is een focus van het lectoraat Zonne Energie in de Gebouwde Omgeving van Zuyd. De toepassing in de glastuinbouw is een focus van de Hogeschool Arnhem Nijmegen in het lectoraat duurzame energie. De onderzoekvraag voor dit project is: “Can we produce nanoparticles with high specificity for use in advanced coatings and polymers with tailored functionalities for application in greenhouses and solar cells using (micro)flow?” De consortiumleden Zuyd Hogeschool / lectoraat material sciences (microreactor technologie / nanotechnologie), TNO/brightlands Material Centre (nanomaterialen voor energietoepassingen), Kriya Materials (producent nanodeeltjes) en Chemtrix (microflow apparatuur) zullen TiO2 en ZnO nanodeeltjes maken en karakteriseren. De consortiumpartners Zuyd / lectoraat Zonne-energie in de duurzaam gebouwde omgevingen HAN (lectoraat duurzame energie) zullen de geproduceerde nanodeeltjes testen in optisch actieve coatings voor toepassingen in zonne-energie en glastuinbouw respectievelijk. De consortiumpartner NanoHouse zal het stuk disseminatie op zich nemen.
The Water Framework Directive imposes challenges regarding the environmental risk of plastic pollution. The quantification, qualification, monitoring, and risk assessment of nanoplastics and small microplastic (<20 µm) is crucial. Environmental nano- and micro-plastics (NMPs) are highly diverse, accounting for this diversity poses a big challenge in developing a comprehensive understanding of NMPs detection, quantification, fate, and risks. Two major issues currently limit progress within this field: (a) validation and broadening the current analytical tools (b) uncertainty with respect to NMPs occurrence and behaviour at small scales (< 20 micron). Tracking NMPs in environmental systems is currently limited to micron size plastics due to the size detection limit of the available analytical techniques. There are currently no methods that can detect nanoplastics in real environmental systems. A major bottleneck is the incompatibility between commercially available NMPs and those generated from plastic fragments degradation in the environment. To track nanoplastics in environmental and biological systems, some research groups synthesized metal-doped nanoplastics, often limited to one polymer type and using high concentrations of surfactants, rendering these synthesized nanoplastics to not be representative of nanoplatics found in real environment. NanoManu proposes using Electrohydrodynamic Atomization to generate metal doped NMPs of different polymers types, sizes, and shapes, which will be representative of the real environmental nanoplastics. The synthesized nanoplastics will be used as model particles in environmental studies. The synthesized nanoplastics will be characterized and tested using different analytical methods, e.g., SEM-EDX, TEX, GCpyrMS, FFF, µFTIR and SP-ICP-MS. NanoManu is a first and critical step towards generating a comprehensive state-of-the-art analytical and environmental knowledge on the environmental fate and risks of nanoplastics. This knowledge impacts current risk assessment tools, efficient interventions to limit emissions and adequate regulations related to NMPs.
