Standard SARS-CoV-2 testing protocols using nasopharyngeal/throat (NP/T) swabs are invasive and require trained medical staff for reliable sampling. In addition, it has been shown that PCR is more sensitive as compared to antigen-based tests. Here we describe the analytical and clinical evaluation of our in-house RNA extraction-free saliva-based molecular assay for the detection of SARS-CoV-2. Analytical sensitivity of the test was equal to the sensitivity obtained in other Dutch diagnostic laboratories that process NP/T swabs. In this study, 955 individuals participated and provided NP/T swabs for routine molecular analysis (with RNA extraction) and saliva for comparison. Our RT-qPCR resulted in a sensitivity of 82,86% and a specificity of 98,94% compared to the gold standard. A false-negative ratio of 1,9% was found. The SARS-CoV-2 detection workflow described here enables easy, economical, and reliable saliva processing, useful for repeated testing of individuals.
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This thesis demonstrates the current situation of Go Free Uganda. According to this recommendations have been formulated to show where the organization can bridge the sales gap. This research makes use of the AIDA model, competitor analysis, Value Proposition Canvas and a Business Model Canvas
MULTIFILE
The Vulkan real estate site in Oslo is owned by Aspelin Ramm, and includes one of the largest parking garages used for EV charging in Europe. EV charging (both AC and DC) is managed for now predominately for costs reasons but also with relevance at further EV penetration level in this car parking location (mixed EV and ICE vehicles). This neighbourhood scale SEEV4-City operational pilot (OP) has 50 22 kW flexible AC chargers with two sockets each and two DC chargers of 50 kW with both ChaDeMo and CCS outlets. All EV chargers now have a smart control (SC) and Vehicle-to-Grid (V2G) functionality (though the latter may not be in place fully for DC chargers, as they may not be fully connected to the remote back-office system of the EV charging systems operator). A Lithium-ion Battery Energy Stationary Storage System (BESS) with a capacity of 50 kWh is pre-programmed to reduce the energy power peaks of the electric vehicle (EV) charging infrastructure and charges at other times from the central grid (which has a generation mix of 98% from hydro-electric power, and in the region covering Oslo also 1% from wind). The inverter used in the BESS is rated at 50 kW, and is also controlled to perform phase balancing of the 3-phase supply system.
Various companies in diagnostic testing struggle with the same “valley of death” challenge. In order to further develop their sensing application, they rely on the technological readiness of easy and reproducible read-out systems. Photonic chips can be very sensitive sensors and can be made application-specific when coated with a properly chosen bio-functionalized layer. Here the challenge lies in the optical coupling of the active components (light source and detector) to the (disposable) photonic sensor chip. For the technology to be commercially viable, the price of the disposable photonic sensor chip should be as low as possible. The coupling of light from the source to the photonic sensor chip and back to the detectors requires a positioning accuracy of less than 1 micrometer, which is a tremendous challenge. In this research proposal, we want to investigate which of the six degrees of freedom (three translational and three rotational) are the most crucial when aligning photonic sensor chips with the external active components. Knowing these degrees of freedom and their respective range we can develop and test an automated alignment tool which can realize photonic sensor chip alignment reproducibly and fully autonomously. The consortium with expertise and contributions in the value chain of photonics interfacing, system and mechanical engineering will investigate a two-step solution. This solution comprises a passive pre-alignment step (a mechanical stop determines the position), followed by an active alignment step (an algorithm moves the source to the optimal position with respect to the chip). The results will be integrated into a demonstrator that performs an automated procedure that aligns a passive photonic chip with a terminal that contains the active components. The demonstrator is successful if adequate optical coupling of the passive photonic chip with the external active components is realized fully automatically, without the need of operator intervention.
The latest IPCC Report (2022) provided by the UN shows us that, to guarantee a safe future for upcoming generations, we must change how we lead our lives on several levels. However, the increasing urgency to act and behave in a way that is not damaging the climate is bringing many psychological concerns to young generations. Worldwide reports are demonstrating how the issue of eco-anxiety is increasing daily, and how young people are feeling more hopeless than ever. Climate change has become a climate crisis, and individuals are experiencing pressure and fear incessantly (Marks et al., 2021). We, as Climate Streamers, have often found ourselves in this situation as well, but rather than freezing, we decided to take this challenge and think of solutions. Therefore, with the support of Breda University of Applied Sciences, the Performatory community, the BUas Startup Support Team, and outside mentors, we created Climate Streamers Foundation: a new youth-led non-profit organisation and a movement working towards a more inclusive and less polarised climate action. By working with leisure elements and a positive and appreciative approach, we want to give back hope, voice and power to the youth and inspire each other genuinely and sustainably. The purpose of this application is to allow us to elaborate a feasibility study concerning our MVP (minimum viable product), the card game, and boost the overall concept. We intend to implement the researched data to improve the design and sales management. The card game aims to stimulate appreciative conversations by giving space to players to express their opinions and personal stories and it is designed so everyone can play it, regardless of background and knowledge. After giving 200 games in production, we launched the card game in July 2022.
In the course of the “energie transitie” hydrogen is likely to become a very important energy carrier. The production of hydrogen (and oxygen) by water electrolysis using electricity from sun or wind is the only sustainable option. Water electrolysis is a well-developed technique, however the production costs of hydrogen by electrolysis are still more expensive than the conventional (not sustainable) production by steam reforming. One challenge towards the large scale application of water electrolysis is the fabrication of stable and cheap (noble metal free) electrodes. In this project we propose to develop fabrication methods for working electrodes and membrane electrode stack (MEAs) that can be used to implement new (noble metal free) electrocatalysts in water electrolysers.
