The decomposition of a body is influenced by burial conditions, making it crucial to understand the impact of different conditions for accurate grave detection. Geophysical techniques using drones have gained popularity in locating clandestine graves, offering non-invasive methods for detecting surface and subsurface irregularities. Ground-penetrating radar (GPR) is an effective technology for identifying potential grave locations without disturbance. This research aimed to prototype a drone system integrating GPR to assist in grave localization and to develop software for data management. Initial experiments compared GPR with other technologies, demonstrating its valuable applicability. It is suitable for various decomposition stages and soil types, although certain soil compositions have limitations. The research used the DJI M600 Pro drone and a drone-based GPR system enhanced by the real-time kinematic (RTK) global positioning system (GPS) for precision and autonomy. Tests with simulated graves and cadavers validated the system’s performance, evaluating optimal altitude, speed, and obstacle avoidance techniques. Furthermore, global and local planning algorithms ensured efficient and obstacle-free flight paths. The results highlighted the potential of the drone-based GPR system in locating clandestine graves while minimizing disturbance, contributing to the development of effective tools for forensic investigations and crime scene analysis.
MULTIFILE
Non-intubated patients with acute respiratory failure due to COVID-19 could benefit from awake proning. Awake proning is an attractive intervention in settings with limited resources, as it comes with no additional costs. However, awake proning remains poorly used probably because of unfamiliarity and uncertainties regarding potential benefits and practical application. To summarize evidence for benefit and to develop a set of pragmatic recommendations for awake proning in patients with COVID-19 pneumonia, focusing on settings where resources are limited, international healthcare professionals from high and low- and middle-income countries (LMICs) with known expertise in awake proning were invited to contribute expert advice. A growing number of observational studies describe the effects of awake proning in patients with COVID-19 pneumonia in whom hypoxemia is refractory to simple measures of supplementary oxygen. Awake proning improves oxygenation in most patients, usually within minutes, and reduces dyspnea and work of breathing. The effects are maintained for up to 1 hour after turning back to supine, and mostly disappear after 6–12 hours. In available studies, awake proning was not associated with a reduction in the rate of intubation for invasive ventilation. Awake proning comes with little complications if properly implemented and monitored. Pragmatic recommendations including indications and contraindications were formulated and adjusted for resource-limited settings. Awake proning, an adjunctive treatment for hypoxemia refractory to supplemental oxygen, seems safe in non-intubated patients with COVID-19 acute respiratory failure. We provide pragmatic recommendations including indications and contraindications for the use of awake proning in LMICs.
Standard SARS-CoV-2 testing protocols using nasopharyngeal/throat (NP/T) swabs are invasive and require trained medical staff for reliable sampling. In addition, it has been shown that PCR is more sensitive as compared to antigen-based tests. Here we describe the analytical and clinical evaluation of our in-house RNA extraction-free saliva-based molecular assay for the detection of SARS-CoV-2. Analytical sensitivity of the test was equal to the sensitivity obtained in other Dutch diagnostic laboratories that process NP/T swabs. In this study, 955 individuals participated and provided NP/T swabs for routine molecular analysis (with RNA extraction) and saliva for comparison. Our RT-qPCR resulted in a sensitivity of 82,86% and a specificity of 98,94% compared to the gold standard. A false-negative ratio of 1,9% was found. The SARS-CoV-2 detection workflow described here enables easy, economical, and reliable saliva processing, useful for repeated testing of individuals.
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