Search results

Improved Efficacy of Oral Immunotherapy Using Non-Digestible Oligosaccharides in a Murine Cow's Milk Allergy Model

From teh UU repository: "Background: Oral immunotherapy (OIT) is a promising therapeutic approach to treat food allergic patients. However, there are some concerns regarding its safety and long-term efficacy. The use of non-digestible oligosaccharides might improve OIT efficacy since they are known to directly modulate intestinal epithelial and immune cells in addition to acting as prebiotics. Aim: To investigate whether a diet supplemented with plant-derived fructo-oligosaccharides (FOS) supports the efficacy of OIT in a murine cow's milk allergy model and to elucidate the potential mechanisms involved. Methods: After oral sensitization to the cow's milk protein whey, female C3H/HeOuJ mice were fed either a control diet or a diet supplemented with FOS (1% w/w) and received OIT (10 mg whey) 5 days a week for 3 weeks by gavage. Intradermal (i.d.) and intragastric (i.g.) challenges were performed to measure acute allergic symptoms and mast cell degranulation. Blood and organs were collected to measure antibody levels and T cell and dendritic cell populations. Spleen-derived T cell fractions (whole spleen-and CD25-depleted) were transferred to naive recipient mice to confirm the involvement of regulatory T cells (Tregs) in allergy protection induced by OIT + FOS. Results: OIT + FOS decreased acute allergic symptoms and mast cell degranulation upon challenge and prevented the challenge-induced increase in whey-specific IgE as observed in sensitized mice. Early induction of Tregs in the mesenteric lymph nodes (MLN) of OIT + FOS mice coincided with reduced T cell responsiveness in splenocyte cultures. CD25 depletion in OIT + FOS-derived splenocyte suspensions prior to transfer abolished protection against signs of anaphylaxis in recipients. OIT + FOS increased serum galectin-9 levels. No differences in short-chain fatty acid (SCFA) levels in the cecum were observed between the treatment groups. Concisely, FOS supplementation significantly improved OIT in the acute allergic skin response, %Foxp3+ Tregs and %LAP+ Th3 cells in MLN, and serum galectin-9 levels. Conclusion: FOS supplementation improved the efficacy of OIT in cow's milk allergic mice. Increased levels of Tregs in the MLN and abolished protection against signs of anaphylaxis upon transfer of CD25-depleted cell fractions, suggest a role for Foxp3+ Tregs in the protective effect of OIT + FOS. "

A prediction model for the demand of charging stations for electric vehicles in the municipality of Amsterdam

Designing digital tooling for business model exploration for the Internet-of-Things

Publicatie ism TU Delft voor Proceedings of DESRIST 2018, 3-6 June, Madras, India. As digital technologies are transforming enterprises, the interest in business models is increasing. Technological disruptions like the Internet of Things (IoT) drive enterprises to redefine their business models to create and capture value, and eventually, to stay competitive. The need for business model innovation may be urgent, yet it is not always clear what to change in a business model. In these cases, business model exploration is needed. Within academia and practice, business model tools are mainly focused on formalizing single business model designs rather than facilitating systematic exploration of alternative business models. In this study, we present the design and prototype of a digital tool created to facilitate business model exploration. We use Design Science Research (DSR) as our research approach. In this paper, we present the results from the first cycle evaluation of the design and prototype.

MultipleXLab: A high-throughput portable live-imaging root phenotyping platform using deep learning and computer vision.

Background: Profiling the plant root architecture is vital for selecting resilient crops that can efficiently take up water and nutrients. The high-performance imaging tools available to study root-growth dynamics with the optimal resolution are costly and stationary. In addition, performing nondestructive high-throughput phenotyping to extract the structural and morphological features of roots remains challenging. Results: We developed the MultipleXLab: a modular, mobile, and cost-effective setup to tackle these limitations. The system can continuously monitor thousands of seeds from germination to root development based on a conventional camera attached to a motorized multiaxis-rotational stage and custom-built 3D-printed plate holder with integrated light-emitting diode lighting. We also developed an image segmentation model based on deep learning that allows the users to analyze the data automatically. We tested the MultipleXLab to monitor seed germination and root growth of Arabidopsis developmental, cell cycle, and auxin transport mutants non-invasively at high-throughput and showed that the system provides robust data and allows precise evaluation of germination index and hourly growth rate between mutants. Conclusion: MultipleXLab provides a flexible and user-friendly root phenotyping platform that is an attractive mobile alternative to high-end imaging platforms and stationary growth chambers. It can be used in numerous applications by plant biologists, the seed industry, crop scientists, and breeding companies.

Profiling of patients with COPD for adequate referral to exercise‑based care

A loss of physical functioning (i.e., a low physical capacity and/or a low physical activity) is a common feature in patients with chronic obstructive pulmonary disease (COPD). To date, the primary care physiotherapy and specialized pulmonary rehabilitation are clearly underused, and limited to patients with a moderate to very severe degree of airflow limitation (GOLD stage 2 or higher). However, improved referral rates are a necessity to lower the burden for patients with COPD and for society. Therefore, a multidisciplinary group of healthcare professionals and scientists proposes a new model for referral of patients with COPD to the right type of exercise-based care, irrespective of the degree of airflow limitation. Indeed, disease instability (recent hospitalization, yes/no), the burden of disease (no/low, mild/moderate or high), physical capacity (low or preserved) and physical activity (low or preserved) need to be used to allocate patients to one of the six distinct patient profiles. Patients with profile 1 or 2 will not be referred for physiotherapy; patients with profiles 3–5 will be referred for primary care physiotherapy; and patients with profile 6 will be referred for screening for specialized pulmonary rehabilitation. The proposed Dutch model has the intention to get the right patient with COPD allocated to the right type of exercise-based care and at the right moment.

A model of low-velocity impact damage assessment of laminated composite structures

Laminated composites have important applications in modern aeronautical structures due to their extraordinary mechanical and environmental behaviour. Nevertheless, aircraft composite structures are highly vulnerable to impact damage, either by low-velocity sources during maintenance or high-velocity sources during in-flight events. Even barely visible impact damage induced by low-velocity loading, substantially reduces the residual mechanical performance and the safe-service life of the composites structures. Despite the extensive research already carried out, impact damage of laminated composite structures is still not well understood and it is an area of on-going research. Numerical modelling is considered as the most efficient tool as compared to the expensive and time-consuming experimental testing. In this paper, a finite element model based on explicit dynamics formulations is adopted. Hashin criterion is applied to predict the intra-laminar damage initiation and evolution. The numerical analysis is performed using the ABAQUS ® programme. The employed modelling approach is validated using numerical results found in the literature and the presented results show an acceptable correlation to the available literature data. It is demonstrated that the presented model is able to capture force-time response as well as damage evolution map for a range of impact energies.