In this paper, we experimentally compare orthogonal frequency-division multiplexing (OFDM) and on-off keying (OOK) modulation in the context of the IEEE 802.15.13-2023 standard at bandwidths up to 50 MHz across a Li-Fi link with distances up to 5 m and a lateral offset up to 51°. Error vector magnitude (EVM) and bit error rate (BER) evaluations confirm that the high peak-to-average power ratio (PAPR) of OFDM limits the achievable transmission distance, but it offers higher data rates due to its higher spectral efficiency. Due to the lower PAPR, OOK-based Pulsed Modulation PHY (PM-PHY) shows a significantly higher link range. As the structure of the PM-PHY is based on OFDM symbols, the two solutions may also be combined to open a wider range of use cases for optical wireless communications.
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tIn this study we aimed to identify genes that are responsive to pertussis toxin (PTx) and might eventu-ally be used as biological markers in a testing strategy to detect residual PTx in vaccines. By microarrayanalysis we screened six human cell types (bronchial epithelial cell line BEAS-2B, fetal lung fibroblastcell line MRC-5, primary cardiac microvascular endothelial cells, primary pulmonary artery smooth mus-cle cells, hybrid cell line EA.Hy926 of umbilical vein endothelial cells and epithelial cell line A549 andimmature monocyte-derived dendritic cells) for differential gene expression induced by PTx. Imma-ture monocyte-derived dendritic cells (iMoDCs) were the only cells in which PTx induced significantdifferential expression of genes. Results were confirmed using different donors and further extendedby showing specificity for PTx in comparison to Escherichia coli lipopolysaccharide (LPS) and Bordetellapertussis lipo-oligosaccharide (LOS). Statistical analysis indicated 6 genes, namely IFNG, IL2, XCL1, CD69,CSF2 and CXCL10, as significantly upregulated by PTx which was also demonstrated at the protein levelfor genes encoding secreted proteins. IL-2 and IFN- gave the strongest response. The minimal PTx con-centrations that induced production of IL-2 and IFN- in iMoDCs were 12.5 and 25 IU/ml, respectively.High concentrations of LPS slightly induced IFN- but not IL-2, while LOS and detoxified pertussis toxindid not induce production of either cytokine. In conclusion, using microarray analysis we evaluated sixhuman cell lines/types for their responsiveness to PTx and found 6 PTx-responsive genes in iMoDCs ofwhich IL2 is the most promising candidate to be used as a biomarker for the detection of residual PTx.
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