Since the Like-button was introduced in 2009 by Facebook, its use increased rapidly. Liking became an important part of our daily routines on the internet since we are able to like almost any creative expression, such as photos and videos. Little is known about the effects of liking (and disliking) on these creative expressions. This paper investigates how the (dis)liking feature influences creativity. We conducted an experiment with a photo-application for mobile phones and provided different combinations of liking and disliking to two research groups and a control group. Our findings suggest thatproviding a liking feature is beneficial to creativity in terms of the production of creative content and results in higher amounts of photos. Providing a disliking feature as well is beneficial to creativity in terms of quality and results in higher rates for the photos.
Immunofluorescence microscopy in this study shows that GLUT-4 protein expression is fibre-type specific within a muscle. It is postulated that both fibre-type-dependent and fibre-type-independent factors affect GLUT-4 expression.
BACKGROUND: Glucocorticoids (GCs) control expression of a large number of genes via binding to the GC receptor (GR). Transcription may be regulated either by binding of the GR dimer to DNA regulatory elements or by protein-protein interactions of GR monomers with other transcription factors. Although the type of regulation for a number of individual target genes is known, the relative contribution of both mechanisms to the regulation of the entire transcriptional program remains elusive. To study the importance of GR dimerization in the regulation of gene expression, we performed gene expression profiling of livers of prednisolone-treated wild type (WT) and mice that have lost the ability to form GR dimers (GRdim).RESULTS: The GR target genes identified in WT mice were predominantly related to glucose metabolism, the cell cycle, apoptosis and inflammation. In GRdim mice, the level of prednisolone-induced gene expression was significantly reduced compared to WT, but not completely absent. Interestingly, for a set of genes, involved in cell cycle and apoptosis processes and strongly related to Foxo3a and p53, induction by prednisolone was completely abolished in GRdim mice. In contrast, glucose metabolism-related genes were still modestly upregulated in GRdim mice upon prednisolone treatment. Finally, we identified several novel GC-inducible genes from which Fam107a, a putative histone acetyltransferase complex interacting protein, was most strongly dependent on GR dimerization.CONCLUSIONS: This study on prednisolone-induced effects in livers of WT and GRdim mice identified a number of interesting candidate genes and pathways regulated by GR dimers and sheds new light onto the complex transcriptional regulation of liver function by GCs.
Synthetic ultra-black (UB) materials, which demonstrate exceptionally high absorbance (>99%) of visible light incident on their surface, are currently used as coatings in photovoltaic cells and numerous other applications. Most commercially available UB coatings are based on an array of carbon nanotubes, which are produced at relatively high temperature and result in numerous by-products. In addition, UB nanotube coatings require harsh application conditions and are very susceptible to abrasion. As a result, these coatings are currently obtained using a manufacturing process with relatively high costs, high energy consumption and low sustainability. Interestingly, an UB coating based on a biologically derived pigment could provide a cheaper and more sustainable alternative. Specifically, GLO Biotics proposes to create UB pigment by taking a bio-mimetic approach and replicate structures found in UB deep-sea fish. A recent study[1] has actually shown that specific fish have melanosomes in their skin with particular dimensions that allow absorption of up to 99.9% of incident light. In addition to this, recent advances in bacterial engineering have demonstrated that it is possible to create bacteria-derived melanin particles with very similar dimensions to the melanosomes in aforementioned fish. During this project, the consortium partners will combine both scientific observations in an attempt to provide the proof-of-concept for developing an ultra-black coating using bacteria-derived melanin particles as bio-based, sustainable pigment. For this, Zuyd University of Applied Sciences (Zuyd) and Maastricht University (UM) collaborate with GLO Biotics in the development of the innovative ‘BLACKTERIA’ UB coating technology. The partners will attempt at engineering an E. coli expression system and adapt its growth in order to produce melanin particles of desired dimensions. In addition, UM will utilize their expertise in industrial coating research to provide input for experimental set-up and the development of a desired UB coating using the bacteria-derived melanin particles as pigment.
CRISPR/Cas genome engineering unleashed a scientific revolution, but entails socio-ethical dilemmas as genetic changes might affect evolution and objections exist against genetically modified organisms. CRISPR-mediated epigenetic editing offers an alternative to reprogram gene functioning long-term, without changing the genetic sequence. Although preclinical studies indicate effective gene expression modulation, long-term effects are unpredictable. This limited understanding of epigenetics and transcription dynamics hampers straightforward applications and prevents full exploitation of epigenetic editing in biotechnological and health/medical applications.Epi-Guide-Edit will analyse existing and newly-generated screening data to predict long-term responsiveness to epigenetic editing (cancer cells, plant protoplasts). Robust rules to achieve long-term epigenetic reprogramming will be distilled based on i) responsiveness to various epigenetic effector domains targeting selected genes, ii) (epi)genetic/chromatin composition before/after editing, and iii) transcription dynamics. Sustained reprogramming will be examined in complex systems (2/3D fibroblast/immune/cancer co-cultures; tomato plants), providing insights for improving tumor/immune responses, skin care or crop breeding. The iterative optimisations of Epi-Guide-Edit rules to non-genetically reprogram eventually any gene of interest will enable exploitation of gene regulation in diverse biological models addressing major societal challenges.The optimally balanced consortium of (applied) universities, ethical and industrial experts facilitates timely socioeconomic impact. Specifically, the developed knowledge/tools will be shared with a wide-spectrum of students/teachers ensuring training of next-generation professionals. Epi-Guide-Edit will thus result in widely applicable effective epigenetic editing tools, whilst training next-generation scientists, and guiding public acceptance.
In the past decade additive manufacturing has gained an incredible traction in the construction industry. The field of 3D concrete printing (3DCP) has advanced significantly, leading to commercially viable housing projects. The use of concrete represents a challenge because of its environmental impact and CO2 footprint. Due to its material properties, structural capacity and ability to take on complex geometries with relative ease, concrete is and will remain for the foreseeable future a key construction material. The framework required for casting concrete, in particular non-orthogonal geometries, is in itself wasteful, not reusable, contributing to its negative environmental impact. Non-standard, complex geometries generally require the use of moulds and subsystems to be produced, leading to wasteful, material-intense manufacturing processes, with high carbon footprints. This research proposal bypasses the use of wasteful scaffolding and moulds, by exploring 3D printing with concrete on reusable substructures made of sand, clay or aggregate. Optimised material depositing strategies for 3DCP will be explored, by making use of algorithmic structural optimisation. This way, material is deposited only where structurally needed, allowing for further reduction of raw-material use. This collaboration between Neutelings Riedijk Architects, Vertico and the Architectural Design and Engineering Chair of the TU Eindhoven, investigates full-scale additive manufacturing of spatially complex 3D-concrete printed components using multi-material support systems (clay, sand and aggregates). These materials can be easily shaped multiple times into substrates with complex geometries, without generating material waste. The 3D concrete printed full-scale prototypes can be used as lightweight façade elements, screens or spatial dividers. To generate waterproof components, the cavities of the extruded lattices can be filled up with lightweight clay or cement. This process allows for the exploration of new aesthetic, creative and circular possibilities, complex geometries and new material expressions in architecture and construction, while reducing raw-material use and waste.
