UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.
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Matrix-assisted laser desorption/ionisation time of-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method for the identification of bacteria from agar media. Direct identification from positive blood cultures should decrease the time to obtaining the result. In this study, three different processing methods for the rapid direct identification of bacteria from positive blood culture bottles were compared. In total, 101 positive aerobe BacT/ALERT bottles were included in this study. Aliquots from all bottles were used for three bacterial processing methods, i.e. the commercially available Bruker's MALDI Sepsityper kit, the commercially available Molzym's MolYsis Basic5 kit and a centrifugation/washing method. In addition, the best method was used to evaluate the possibility of MALDI application after a reduced incubation time of 7 h of Staphylococcus aureus- and Escherichia coli-spiked (1,000, 100 and 10 colony-forming units [CFU]) aerobe BacT/ALERT blood cultures. Sixty-six (65%), 51 (50.5%) and 79 (78%) bottles were identified correctly at the species level when the centrifugation/washing method, MolYsis Basic 5 and Sepsityper were used, respectively. Incorrect identification was obtained in 35 (35%), 50 (49.5%) and 22 (22%) bottles, respectively. Gram-positive cocci were correctly identified in 33/52 (64%) of the cases. However, Gram-negative rods showed a correct identification in 45/47 (96%) of all bottles when the Sepsityper kit was used. Seven hours of pre-incubation of S. aureus- and E. coli-spiked aerobe BacT/ALERT blood cultures never resulted in reliable identification with MALDI-TOF MS. Sepsityper is superior for the direct identification of microorganisms from aerobe BacT/ALERT bottles. Gram-negative pathogens show better results compared to Gram-positive bacteria. Reduced incubation followed by MALDI-TOF MS did not result in faster reliable identification.
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Plastic is one of the biggest contributors to pollution of the planet. Due to the low recyclability of oil-based plastics, most plastic is being disposed into the environment. According to plastic oceans, 10 million tons of plastic are dumped into oceans annually. Currently, researchers are developing recycling methods for oil-based plastics and are looking for biobased alternatives. One of these alternatives are a class of polymers called polyhydroxyalkanoates (PHA’s). PHA’s differ from other biobased polymers, due to the process of fabrication. PHA’s are a natural polymer, acting as an energy and carbon storage for different strains of bacteria. Functioning as an energy storage, nature can break down PHA’s and PHA-based waste. (1) Different companies are working on PHA’s production, but a large deviations in physical properties were observed. This research aims to establish a relationship between the chemical and physical properties of the different PHA’s, using gel permeability chromatography (GPC), nuclear magnetic resonance (NMR) and gas chromatography-mass spectroscopy (GC-MS).
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As the Dutch population is aging, the field of music-in-healthcare keeps expanding. Healthcare, institutionally and at home, is multiprofessional and demands interprofessional collaboration. Musicians are sought-after collaborators in social and healthcare fields, yet lesser-known agents of this multiprofessional group. Although live music supports social-emotional wellbeing and vitality, and nurtures compassionate care delivery, interprofessional collaboration between musicians, social work, and healthcare professionals remains marginal. This limits optimising and integrating music-making in the care. A significant part of this problem is a lack of collaborative transdisciplinary education for music, social, and healthcare students that deep-dives into the development of interprofessional skills. To meet the growing demand for musical collaborations by particularly elderly care organisations, and to innovate musical contributions to the quality of social and healthcare in Northern Netherlands, a transdisciplinary education for music, physiotherapy, and social work studies is needed. This project aims to equip multiprofessional student groups of Hanze with interprofessional skills through co-creative transdisciplinary learning aimed at innovating and improving musical collaborative approaches for working with vulnerable, often older people. The education builds upon experiential learning in Learning LABs, and collaborative project work in real-life care settings, supported by transdisciplinary community forming.The expected outcomes include a new concept of a transdisciplinary education for HBO-curricula, concrete building blocks for a transdisciplinary arts-in-health minor study, innovative student-led approaches for supporting the care and wellbeing of (older) vulnerable people, enhanced integration of musicians in interprofessional care teams, and new interprofessional structures for educational collaboration between music, social work and healthcare faculties.
In tijden van toenemende culturele diversiteit en arbeidsonzekerheid hebben jongeren in Nederlandse en Duitse stadswijken grote behoefte aan richting met betrekking tot hun toekomstige leven. Ouders en leraren lijken zelf vaak te worden overweldigd door de snel veranderende wereld waarin ze leven. Naast deze veranderingen neemt het gebruik van sociale media sterk toe, waardoor de al bestaande generatiekloof nog groter wordt. Deze ontwikkelingen hebben grote gevolgen voor de levensloopperspectieven van jongeren en leiden er vaak toe dat ze meer dan ooit richting zoeken bij hun leeftijdgenoten. In plaats van dit te zien als een problematische situatie, is dit project erop gericht de netwerken van jongeren te gebruiken als bron voor verbetering van de stadswijken. Het basisidee is jonge adolescenten (in de leeftijd van 12-14 jaar) te empoweren via bepaalde leeftijdgenoten die al gerespecteerd, verantwoordelijk en stabiel in het leven staan. Deze ‘homies’ (vier Nederlandse en vier Duitse jongeren) worden getraind en begeleid door experts op het gebied van oplossingsgericht denken en inspirerende communicatie. Daarna gaan de homies aan de slag in hun eigen wijk, waar ze drie maanden actief zullen zijn. De meeste communicatie met hun leeftijdgenoten zal verlopen via mobiele communicatie en sociale medianetwerken. In het begeleidende onderzoek wordt een analyse gemaakt van de leefsituatie van jongeren in de geselecteerde wijken voor en na de tussenkomst van de homies. De homies houden zelf een (mobiel) dagboek bij dat inzicht zal bieden in hoe zij zelf de veranderingen bij de jongeren in hun wijk zien.
Centre of Expertise, part of Hogeschool Rotterdam, Hogeschool van Arnhem en Nijmegen, Fontys
Centre of Expertise, part of Avans Hogeschool
Centre of Expertise, part of Hogeschool iPabo, Amsterdamse Hogeschool voor de Kunsten, Avans Hogeschool, +3
